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Analysis of the involvement of cytokines in allergy and breast cancer association.

Kowalczewska ME, Brożyna A, Jóźwicki W, Pławski K, Przybyszewski M, Wrotek S, Bartuzi Z, Kozak W - Contemp Oncol (Pozn) (2014)

Bottom Line: Subsequent molecular analysis, however, did not show statistically significant differences in cytokines mRNA and protein expression levels between allergic patients and those with malignancy.The in vitro reactivity test also did not reveal marked differences between IL-1β, IL-4 and IL-6 production after PBMC triggering with exogenous antigen.We concluded that the studied cytokines (IL-1β, IL-4, IL-6, and IFN-γ) are not engaged in breast cancer-allergy negative relation.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology Faculty of Biology and Environment Protection Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Aim of the study: The existence of a correlation between allergy disorders and cancer diseases has been confirmed by several epidemiological studies. Although the molecular mechanism involved in this phenomenon remains unknown, there are data indicating that certain cytokines, engaged in allergic processes, have antineoplastic activities. The aim of the present study was to explore the association between advanced breast cancer and allergic state on the molecular level.

Material and methods: We determined and compared the mRNA and protein expression of interleukin-1β (IL-1β), IL-4, IL-6, and interferon-γ (IFN-γ), cytokines known for antitumor properties, in the blood of advanced breast cancer patients and individuals with allergic diseases related to type 2 response. In addition, we performed an in vitro assay of reactivity of peripheral blood mononuclear cells after exogenous antigen stimulation. As a preliminary to molecular analysis we conducted a questionnaire study concerning the incidence of allergy among breast cancer patients and healthy subjects without malignancy.

Results: The results of the survey study revealed a negative relation between breast cancer and allergy prevalence. Subsequent molecular analysis, however, did not show statistically significant differences in cytokines mRNA and protein expression levels between allergic patients and those with malignancy. The in vitro reactivity test also did not reveal marked differences between IL-1β, IL-4 and IL-6 production after PBMC triggering with exogenous antigen.

Conclusions: We concluded that the studied cytokines (IL-1β, IL-4, IL-6, and IFN-γ) are not engaged in breast cancer-allergy negative relation.

No MeSH data available.


Related in: MedlinePlus

Changes in IL-1β concentration after PBMC in vitro LPS stimulation (abbreviations: control – healthy individuals, n = 3; cancer – breast cancer patients, n = 3; allergy – Th2 allergic individuals, n = 3). Values are expressed as means ± S.E.M. of all samples within the groups. Asterisk indicates significant difference between groups (**p < 0.01, Student's t-test)
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Figure 0006: Changes in IL-1β concentration after PBMC in vitro LPS stimulation (abbreviations: control – healthy individuals, n = 3; cancer – breast cancer patients, n = 3; allergy – Th2 allergic individuals, n = 3). Values are expressed as means ± S.E.M. of all samples within the groups. Asterisk indicates significant difference between groups (**p < 0.01, Student's t-test)

Mentions: Stimulation of PBMC with LPS significantly increased the production of IL-1β, IL-4, and IL-6 compared to those incubated with NaCl as a control (Fig. 6, 7, 8). In addition, we observed a marked increase in IL-1β, IL-4, and IL-6 production after LPS stimulation of PBMC isolated from the blood of neoplastic and allergic patients compared to healthy controls. However there were no significant differences in supernatant levels of these cytokines between breast cancer and allergic samples in response to the exogenous antigen.


Analysis of the involvement of cytokines in allergy and breast cancer association.

Kowalczewska ME, Brożyna A, Jóźwicki W, Pławski K, Przybyszewski M, Wrotek S, Bartuzi Z, Kozak W - Contemp Oncol (Pozn) (2014)

Changes in IL-1β concentration after PBMC in vitro LPS stimulation (abbreviations: control – healthy individuals, n = 3; cancer – breast cancer patients, n = 3; allergy – Th2 allergic individuals, n = 3). Values are expressed as means ± S.E.M. of all samples within the groups. Asterisk indicates significant difference between groups (**p < 0.01, Student's t-test)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355658&req=5

Figure 0006: Changes in IL-1β concentration after PBMC in vitro LPS stimulation (abbreviations: control – healthy individuals, n = 3; cancer – breast cancer patients, n = 3; allergy – Th2 allergic individuals, n = 3). Values are expressed as means ± S.E.M. of all samples within the groups. Asterisk indicates significant difference between groups (**p < 0.01, Student's t-test)
Mentions: Stimulation of PBMC with LPS significantly increased the production of IL-1β, IL-4, and IL-6 compared to those incubated with NaCl as a control (Fig. 6, 7, 8). In addition, we observed a marked increase in IL-1β, IL-4, and IL-6 production after LPS stimulation of PBMC isolated from the blood of neoplastic and allergic patients compared to healthy controls. However there were no significant differences in supernatant levels of these cytokines between breast cancer and allergic samples in response to the exogenous antigen.

Bottom Line: Subsequent molecular analysis, however, did not show statistically significant differences in cytokines mRNA and protein expression levels between allergic patients and those with malignancy.The in vitro reactivity test also did not reveal marked differences between IL-1β, IL-4 and IL-6 production after PBMC triggering with exogenous antigen.We concluded that the studied cytokines (IL-1β, IL-4, IL-6, and IFN-γ) are not engaged in breast cancer-allergy negative relation.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology Faculty of Biology and Environment Protection Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Aim of the study: The existence of a correlation between allergy disorders and cancer diseases has been confirmed by several epidemiological studies. Although the molecular mechanism involved in this phenomenon remains unknown, there are data indicating that certain cytokines, engaged in allergic processes, have antineoplastic activities. The aim of the present study was to explore the association between advanced breast cancer and allergic state on the molecular level.

Material and methods: We determined and compared the mRNA and protein expression of interleukin-1β (IL-1β), IL-4, IL-6, and interferon-γ (IFN-γ), cytokines known for antitumor properties, in the blood of advanced breast cancer patients and individuals with allergic diseases related to type 2 response. In addition, we performed an in vitro assay of reactivity of peripheral blood mononuclear cells after exogenous antigen stimulation. As a preliminary to molecular analysis we conducted a questionnaire study concerning the incidence of allergy among breast cancer patients and healthy subjects without malignancy.

Results: The results of the survey study revealed a negative relation between breast cancer and allergy prevalence. Subsequent molecular analysis, however, did not show statistically significant differences in cytokines mRNA and protein expression levels between allergic patients and those with malignancy. The in vitro reactivity test also did not reveal marked differences between IL-1β, IL-4 and IL-6 production after PBMC triggering with exogenous antigen.

Conclusions: We concluded that the studied cytokines (IL-1β, IL-4, IL-6, and IFN-γ) are not engaged in breast cancer-allergy negative relation.

No MeSH data available.


Related in: MedlinePlus