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Overexpression of Raf-1 in basal-like carcinoma of the breast: correlation with clinicopathology and prognosis.

Wu P, Li X, Wu Y, Hu W, Wang Y, Gao L, Chen Z, Zheng W - Contemp Oncol (Pozn) (2014)

Bottom Line: The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05).Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Huaian Maternity and Child Health Hospital, Huaian, Jiangsu Province, China.

ABSTRACT

Aim of the study: Increased Raf-1 expression has been associated with an aggressive behaviour in some carcinomas such as pulmonary carcinoma and renal carcinoma. However, its role in breast cancer, especially in basal-like carcinoma of the breast (BLBC), has not been defined.

Material and methods: The current study attempted to investigate the expression pattern of Raf-1 protein in BLBC, in relation to the biological behaviour and prognosis of the carcinoma. Expression of Raf-1 was detected by immunohistochemistry in carcinoma specimens from 74 cases of BLBC, and associations between their expression and the clinicopathological characteristics were statistically assessed.

Results: The patients' age, tumour size, BRCA1, and p53 protein expression was not significantly different between the Raf-1-positive and Raf-1-negative expression groups (p > 0.05). The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05). However, positive cytoplasmic Raf-1 expression was positively correlated to Ki-67 expression (p < 0.05). Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).

Conclusions: The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

No MeSH data available.


Related in: MedlinePlus

Kaplan-Meier analysis of the probability of overall survival based on Raf-1 expressions in BLBC
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Figure 0002: Kaplan-Meier analysis of the probability of overall survival based on Raf-1 expressions in BLBC

Mentions: The relationship between Raf-1 and other clinicopathological variables is summarised in Table 1. No significant correlation was observed in age, tumour size, histologic grade, BRCA1, and p53 protein expression between the positive and negative Raf-1 expression group of BLBC (p > 0.05). The proportion of histological grade 3 tumours was not significantly higher in the Raf-1-positive group than that of grade 2 tumours (p > 0.05). The percentage of tumours with higher Ki-67 expression (≥ 10%) was also higher in the Raf-1-positive group than in the Raf-1-negative group (73.5% vs. 26.5%, p < 0.05). Since an increase in Raf-1 expression was associated with tumour metastasis in axillary lymph nodes, we examined which factor would contribute most significantly to a reduction in five-year disease-free survival (DFS). We performed Cox regression with multivariate analysis to identify the RR of reducing the five-year DFS. Table 2 shows that the following factors contribute significantly to a decrease in DFS: axillary lymph node metastasis and the tumours with higher Ki-67 expression and Raf-1 expression (≥ 10% positivity). Most interestingly, in addition to these traditional risk factors, higher Raf-1 expression was significantly associated with a decrease in five-year DFS (Fig. 2).


Overexpression of Raf-1 in basal-like carcinoma of the breast: correlation with clinicopathology and prognosis.

Wu P, Li X, Wu Y, Hu W, Wang Y, Gao L, Chen Z, Zheng W - Contemp Oncol (Pozn) (2014)

Kaplan-Meier analysis of the probability of overall survival based on Raf-1 expressions in BLBC
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355656&req=5

Figure 0002: Kaplan-Meier analysis of the probability of overall survival based on Raf-1 expressions in BLBC
Mentions: The relationship between Raf-1 and other clinicopathological variables is summarised in Table 1. No significant correlation was observed in age, tumour size, histologic grade, BRCA1, and p53 protein expression between the positive and negative Raf-1 expression group of BLBC (p > 0.05). The proportion of histological grade 3 tumours was not significantly higher in the Raf-1-positive group than that of grade 2 tumours (p > 0.05). The percentage of tumours with higher Ki-67 expression (≥ 10%) was also higher in the Raf-1-positive group than in the Raf-1-negative group (73.5% vs. 26.5%, p < 0.05). Since an increase in Raf-1 expression was associated with tumour metastasis in axillary lymph nodes, we examined which factor would contribute most significantly to a reduction in five-year disease-free survival (DFS). We performed Cox regression with multivariate analysis to identify the RR of reducing the five-year DFS. Table 2 shows that the following factors contribute significantly to a decrease in DFS: axillary lymph node metastasis and the tumours with higher Ki-67 expression and Raf-1 expression (≥ 10% positivity). Most interestingly, in addition to these traditional risk factors, higher Raf-1 expression was significantly associated with a decrease in five-year DFS (Fig. 2).

Bottom Line: The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05).Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Huaian Maternity and Child Health Hospital, Huaian, Jiangsu Province, China.

ABSTRACT

Aim of the study: Increased Raf-1 expression has been associated with an aggressive behaviour in some carcinomas such as pulmonary carcinoma and renal carcinoma. However, its role in breast cancer, especially in basal-like carcinoma of the breast (BLBC), has not been defined.

Material and methods: The current study attempted to investigate the expression pattern of Raf-1 protein in BLBC, in relation to the biological behaviour and prognosis of the carcinoma. Expression of Raf-1 was detected by immunohistochemistry in carcinoma specimens from 74 cases of BLBC, and associations between their expression and the clinicopathological characteristics were statistically assessed.

Results: The patients' age, tumour size, BRCA1, and p53 protein expression was not significantly different between the Raf-1-positive and Raf-1-negative expression groups (p > 0.05). The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05). However, positive cytoplasmic Raf-1 expression was positively correlated to Ki-67 expression (p < 0.05). Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).

Conclusions: The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

No MeSH data available.


Related in: MedlinePlus