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Overexpression of Raf-1 in basal-like carcinoma of the breast: correlation with clinicopathology and prognosis.

Wu P, Li X, Wu Y, Hu W, Wang Y, Gao L, Chen Z, Zheng W - Contemp Oncol (Pozn) (2014)

Bottom Line: The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05).Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Huaian Maternity and Child Health Hospital, Huaian, Jiangsu Province, China.

ABSTRACT

Aim of the study: Increased Raf-1 expression has been associated with an aggressive behaviour in some carcinomas such as pulmonary carcinoma and renal carcinoma. However, its role in breast cancer, especially in basal-like carcinoma of the breast (BLBC), has not been defined.

Material and methods: The current study attempted to investigate the expression pattern of Raf-1 protein in BLBC, in relation to the biological behaviour and prognosis of the carcinoma. Expression of Raf-1 was detected by immunohistochemistry in carcinoma specimens from 74 cases of BLBC, and associations between their expression and the clinicopathological characteristics were statistically assessed.

Results: The patients' age, tumour size, BRCA1, and p53 protein expression was not significantly different between the Raf-1-positive and Raf-1-negative expression groups (p > 0.05). The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05). However, positive cytoplasmic Raf-1 expression was positively correlated to Ki-67 expression (p < 0.05). Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).

Conclusions: The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

No MeSH data available.


Related in: MedlinePlus

Positive Raf-1 expression was located mainly in the cytoplasm of tumour cells of BLBC by immunohistochemical staining (haematoxylin counterstain)
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Figure 0001: Positive Raf-1 expression was located mainly in the cytoplasm of tumour cells of BLBC by immunohistochemical staining (haematoxylin counterstain)

Mentions: In the current immunohistochemical analysis of 74 BLBC cases, the tumour cells showed cytoplasmic staining for Raf-1 protein (Fig. 1). No specific immunoreactivity was detected in these negative control sections. Positive expression of Raf-1 proteins was observed in 46 cases of BLBC (46/74, 62.2%). In the current study, Raf-1 expression was confined to the invasive tumour components, with no staining in normal breast epithelial cells.


Overexpression of Raf-1 in basal-like carcinoma of the breast: correlation with clinicopathology and prognosis.

Wu P, Li X, Wu Y, Hu W, Wang Y, Gao L, Chen Z, Zheng W - Contemp Oncol (Pozn) (2014)

Positive Raf-1 expression was located mainly in the cytoplasm of tumour cells of BLBC by immunohistochemical staining (haematoxylin counterstain)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355656&req=5

Figure 0001: Positive Raf-1 expression was located mainly in the cytoplasm of tumour cells of BLBC by immunohistochemical staining (haematoxylin counterstain)
Mentions: In the current immunohistochemical analysis of 74 BLBC cases, the tumour cells showed cytoplasmic staining for Raf-1 protein (Fig. 1). No specific immunoreactivity was detected in these negative control sections. Positive expression of Raf-1 proteins was observed in 46 cases of BLBC (46/74, 62.2%). In the current study, Raf-1 expression was confined to the invasive tumour components, with no staining in normal breast epithelial cells.

Bottom Line: The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05).Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Huaian Maternity and Child Health Hospital, Huaian, Jiangsu Province, China.

ABSTRACT

Aim of the study: Increased Raf-1 expression has been associated with an aggressive behaviour in some carcinomas such as pulmonary carcinoma and renal carcinoma. However, its role in breast cancer, especially in basal-like carcinoma of the breast (BLBC), has not been defined.

Material and methods: The current study attempted to investigate the expression pattern of Raf-1 protein in BLBC, in relation to the biological behaviour and prognosis of the carcinoma. Expression of Raf-1 was detected by immunohistochemistry in carcinoma specimens from 74 cases of BLBC, and associations between their expression and the clinicopathological characteristics were statistically assessed.

Results: The patients' age, tumour size, BRCA1, and p53 protein expression was not significantly different between the Raf-1-positive and Raf-1-negative expression groups (p > 0.05). The proportion of histological grade 3 tumours was not significantly higher in the Raf-1 positive group than that of grade 2 tumours (p > 0.05). However, positive cytoplasmic Raf-1 expression was positively correlated to Ki-67 expression (p < 0.05). Also, increased Raf-1 protein was found to exert an unfavourable impact on patients' axillary lymph node metastasis and overall survival (p < 0.05).

Conclusions: The study implies that positive Raf-1 expression in BLBC is associated with a more aggressive phenotype and could be considered as a new prognostic biomarker for poor survival in BLBC patients.

No MeSH data available.


Related in: MedlinePlus