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Identification of novel QTL governing root architectural traits in an interspecific soybean population.

Manavalan LP, Prince SJ, Musket TA, Chaky J, Deshmukh R, Vuong TD, Song L, Cregan PB, Nelson JC, Shannon JG, Specht JE, Nguyen HT - PLoS ONE (2015)

Bottom Line: Dunbar (PI 552538) and wild G. soja (PI 326582A) exhibited significant differences in root architecture and root-related traits.This QTL region was found to control various shoot- and root-related traits across soybean genetic backgrounds.It appears that the marker interval Satt315-I locus on chromosome 8 contain an essential QTL contributing to early root and shoot growth in soybean.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America.

ABSTRACT
Cultivated soybean (Glycine max L.) cv. Dunbar (PI 552538) and wild G. soja (PI 326582A) exhibited significant differences in root architecture and root-related traits. In this study, phenotypic variability for root traits among 251 BC2F5 backcross inbred lines (BILs) developed from the cross Dunbar/PI 326582A were identified. The root systems of the parents and BILs were evaluated in controlled environmental conditions using a cone system at seedling stage. The G. max parent Dunbar contributed phenotypically favorable alleles at a major quantitative trait locus on chromosome 8 (Satt315-I locus) that governed root traits (tap root length and lateral root number) and shoot length. This QTL accounted for >10% of the phenotypic variation of both tap root and shoot length. This QTL region was found to control various shoot- and root-related traits across soybean genetic backgrounds. Within the confidence interval of this region, eleven transcription factors (TFs) were identified. Based on RNA sequencing and Affymetrix expression data, key TFs including MYB, AP2-EREBP and bZIP TFs were identified in this QTL interval with high expression in roots and nodules. The backcross inbred lines with different parental allelic combination showed different expression pattern for six transcription factors selected based on their expression pattern in root tissues. It appears that the marker interval Satt315-I locus on chromosome 8 contain an essential QTL contributing to early root and shoot growth in soybean.

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Variation for seed and plant architectural traits in soybean and the BIL population.a) Variation in root architecture in soybean accessions 12 days after sowing (das); b) Seed coat color of parents (Dunbar, P1 and PI 326582A, P2) and RILs c) Cone system for root screening d) Dunbar 12 das e) PI 326582A 12 das.
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pone.0120490.g001: Variation for seed and plant architectural traits in soybean and the BIL population.a) Variation in root architecture in soybean accessions 12 days after sowing (das); b) Seed coat color of parents (Dunbar, P1 and PI 326582A, P2) and RILs c) Cone system for root screening d) Dunbar 12 das e) PI 326582A 12 das.

Mentions: This study utilized a backcross-derived inbred line (BIL) mapping population, created by mating the G. max maturity group III soybean cv. ‘Dunbar’ (PI 552538) with a G. soja maturity group II so-called “wild” soybean accession (PI326582A). The phenotypic descriptors of the parental lines are presented in Table 1. The phenotypic variation of root traits in G. soja, PI326582A is shown in comparison to other soybean accessions in Fig. 1a. The segregation of seed size and color in Dunbar/PI326582A population is shown in Fig. 1b. The F1 plants were backcrossed to the Dunbar parent and the resulting 300+ BC1F1 plants were independently backcrossed again to the Dunbar parent to produce more than 300+ BC2F1 plants. Plant to progeny row (not single-seed descent) was used for generation advancement from the BC2F1 to the BC2F4 generation from which 296 BC2F4.5 progeny rows were separately harvested to produce F4-derived F5 inbred lines, henceforth referred to as BILs.


Identification of novel QTL governing root architectural traits in an interspecific soybean population.

Manavalan LP, Prince SJ, Musket TA, Chaky J, Deshmukh R, Vuong TD, Song L, Cregan PB, Nelson JC, Shannon JG, Specht JE, Nguyen HT - PLoS ONE (2015)

Variation for seed and plant architectural traits in soybean and the BIL population.a) Variation in root architecture in soybean accessions 12 days after sowing (das); b) Seed coat color of parents (Dunbar, P1 and PI 326582A, P2) and RILs c) Cone system for root screening d) Dunbar 12 das e) PI 326582A 12 das.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355624&req=5

pone.0120490.g001: Variation for seed and plant architectural traits in soybean and the BIL population.a) Variation in root architecture in soybean accessions 12 days after sowing (das); b) Seed coat color of parents (Dunbar, P1 and PI 326582A, P2) and RILs c) Cone system for root screening d) Dunbar 12 das e) PI 326582A 12 das.
Mentions: This study utilized a backcross-derived inbred line (BIL) mapping population, created by mating the G. max maturity group III soybean cv. ‘Dunbar’ (PI 552538) with a G. soja maturity group II so-called “wild” soybean accession (PI326582A). The phenotypic descriptors of the parental lines are presented in Table 1. The phenotypic variation of root traits in G. soja, PI326582A is shown in comparison to other soybean accessions in Fig. 1a. The segregation of seed size and color in Dunbar/PI326582A population is shown in Fig. 1b. The F1 plants were backcrossed to the Dunbar parent and the resulting 300+ BC1F1 plants were independently backcrossed again to the Dunbar parent to produce more than 300+ BC2F1 plants. Plant to progeny row (not single-seed descent) was used for generation advancement from the BC2F1 to the BC2F4 generation from which 296 BC2F4.5 progeny rows were separately harvested to produce F4-derived F5 inbred lines, henceforth referred to as BILs.

Bottom Line: Dunbar (PI 552538) and wild G. soja (PI 326582A) exhibited significant differences in root architecture and root-related traits.This QTL region was found to control various shoot- and root-related traits across soybean genetic backgrounds.It appears that the marker interval Satt315-I locus on chromosome 8 contain an essential QTL contributing to early root and shoot growth in soybean.

View Article: PubMed Central - PubMed

Affiliation: Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America.

ABSTRACT
Cultivated soybean (Glycine max L.) cv. Dunbar (PI 552538) and wild G. soja (PI 326582A) exhibited significant differences in root architecture and root-related traits. In this study, phenotypic variability for root traits among 251 BC2F5 backcross inbred lines (BILs) developed from the cross Dunbar/PI 326582A were identified. The root systems of the parents and BILs were evaluated in controlled environmental conditions using a cone system at seedling stage. The G. max parent Dunbar contributed phenotypically favorable alleles at a major quantitative trait locus on chromosome 8 (Satt315-I locus) that governed root traits (tap root length and lateral root number) and shoot length. This QTL accounted for >10% of the phenotypic variation of both tap root and shoot length. This QTL region was found to control various shoot- and root-related traits across soybean genetic backgrounds. Within the confidence interval of this region, eleven transcription factors (TFs) were identified. Based on RNA sequencing and Affymetrix expression data, key TFs including MYB, AP2-EREBP and bZIP TFs were identified in this QTL interval with high expression in roots and nodules. The backcross inbred lines with different parental allelic combination showed different expression pattern for six transcription factors selected based on their expression pattern in root tissues. It appears that the marker interval Satt315-I locus on chromosome 8 contain an essential QTL contributing to early root and shoot growth in soybean.

Show MeSH