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Correlation between genetic variations and serum level of interleukin 28B with virus genotypes and disease progression in chronic hepatitis C virus infection.

Al-Qahtani A, Al-Anazi M, Abdo AA, Sanai FM, Al-Hamoudi W, Alswat KA, Al-Ashgar HI, Khan MQ, Albenmousa A, Khalaf N, Viswan N, Al-Ahdal MN - J Immunol Res (2015)

Bottom Line: Also, patients who were presented with cirrhosis (Cirr) only or with cirrhosis plus hepatocellular carcinoma (Cirr+HCC) had higher levels of serum IL28B when compared to chronic HCV-infected patients (P = 0.005 and 0.003, resp.).This study indicates that variation at SNP rs8099917 could predict the serum levels of IL28B in HCV-infected patients.Furthermore, IL28B serum level may serve as a useful marker for the development of HCV-associated sequelae.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia ; Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia.

ABSTRACT
Recent studies have demonstrated that polymorphisms near the interleukin-28B (IL-28B) gene could predict the response to Peg-IFN-a/RBV combination therapy in HCV-infected patients. The aim of the study was to correlate the serum level of IL28B in HCV-infected patients with virus genotype/subgenotype and disease progression. IL28B serum level was detected and variations at five single nucleotide polymorphisms (SNPs) in IL28B gene region were genotyped and analyzed. The variation of IL28B genetic polymorphisms was found to be strongly associated with HCV infection when healthy control group was compared to HCV-infected patients with all P values <0.0001. Functional analysis revealed that subjects carrying rs8099917-GG genotype had higher serum level of IL28B than those with GT or TT genotypes (P = 0.04). Also, patients who were presented with cirrhosis (Cirr) only or with cirrhosis plus hepatocellular carcinoma (Cirr+HCC) had higher levels of serum IL28B when compared to chronic HCV-infected patients (P = 0.005 and 0.003, resp.). No significant association was found when serum levels of IL28B were compared to virus genotypes/subgenotypes. This study indicates that variation at SNP rs8099917 could predict the serum levels of IL28B in HCV-infected patients. Furthermore, IL28B serum level may serve as a useful marker for the development of HCV-associated sequelae.

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Related in: MedlinePlus

Boxplot analysis of average serum IL28B concentration with different outcomes of HCV infection.
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Related In: Results  -  Collection


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fig2: Boxplot analysis of average serum IL28B concentration with different outcomes of HCV infection.

Mentions: A similar analysis was done to estimate the correlation of serum IL28B levels with regard to different groups of patients classified according to their severity of the disease. A significant result was obtained when group 2 and groups 2+3 were compared to group 1 with an increase of 0.16 in log10 of IL28B serum level for group 2 and group 2+3 samples, while no additional significant effect was observed for group 3 alone (Figure 2 and Table 6).


Correlation between genetic variations and serum level of interleukin 28B with virus genotypes and disease progression in chronic hepatitis C virus infection.

Al-Qahtani A, Al-Anazi M, Abdo AA, Sanai FM, Al-Hamoudi W, Alswat KA, Al-Ashgar HI, Khan MQ, Albenmousa A, Khalaf N, Viswan N, Al-Ahdal MN - J Immunol Res (2015)

Boxplot analysis of average serum IL28B concentration with different outcomes of HCV infection.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4355603&req=5

fig2: Boxplot analysis of average serum IL28B concentration with different outcomes of HCV infection.
Mentions: A similar analysis was done to estimate the correlation of serum IL28B levels with regard to different groups of patients classified according to their severity of the disease. A significant result was obtained when group 2 and groups 2+3 were compared to group 1 with an increase of 0.16 in log10 of IL28B serum level for group 2 and group 2+3 samples, while no additional significant effect was observed for group 3 alone (Figure 2 and Table 6).

Bottom Line: Also, patients who were presented with cirrhosis (Cirr) only or with cirrhosis plus hepatocellular carcinoma (Cirr+HCC) had higher levels of serum IL28B when compared to chronic HCV-infected patients (P = 0.005 and 0.003, resp.).This study indicates that variation at SNP rs8099917 could predict the serum levels of IL28B in HCV-infected patients.Furthermore, IL28B serum level may serve as a useful marker for the development of HCV-associated sequelae.

View Article: PubMed Central - PubMed

Affiliation: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia ; Liver Disease Research Center, King Saud University, Riyadh, Saudi Arabia.

ABSTRACT
Recent studies have demonstrated that polymorphisms near the interleukin-28B (IL-28B) gene could predict the response to Peg-IFN-a/RBV combination therapy in HCV-infected patients. The aim of the study was to correlate the serum level of IL28B in HCV-infected patients with virus genotype/subgenotype and disease progression. IL28B serum level was detected and variations at five single nucleotide polymorphisms (SNPs) in IL28B gene region were genotyped and analyzed. The variation of IL28B genetic polymorphisms was found to be strongly associated with HCV infection when healthy control group was compared to HCV-infected patients with all P values <0.0001. Functional analysis revealed that subjects carrying rs8099917-GG genotype had higher serum level of IL28B than those with GT or TT genotypes (P = 0.04). Also, patients who were presented with cirrhosis (Cirr) only or with cirrhosis plus hepatocellular carcinoma (Cirr+HCC) had higher levels of serum IL28B when compared to chronic HCV-infected patients (P = 0.005 and 0.003, resp.). No significant association was found when serum levels of IL28B were compared to virus genotypes/subgenotypes. This study indicates that variation at SNP rs8099917 could predict the serum levels of IL28B in HCV-infected patients. Furthermore, IL28B serum level may serve as a useful marker for the development of HCV-associated sequelae.

Show MeSH
Related in: MedlinePlus