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Investigating the pretreatment miRNA expression patterns of advanced hepatocellular carcinoma patients in association with response to TACE treatment.

El-Halawany MS, Ismail HM, Zeeneldin AA, Elfiky A, Tantawy M, Kobaisi MH, Hamed I, Abdel Wahab AH - Biomed Res Int (2015)

Bottom Line: Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples.Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year.These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, Faculty of Science, Cairo University, El-Gamaa Street, Giza 12613, Egypt.

ABSTRACT
Hepatocellular carcinoma (HCC) is a lethal malignancy with poor prognosis and limited treatment options. Transarterial chemoembolization (TACE) using chemotherapy agents-doxorubicin and cisplatin-is an accepted treatment option for locally advanced hepatocellular carcinoma. In the current study, we analyzed the expression pattern of a selected panel of 94 miRNAs in archival samples that were collected prior to treatment from 15 Egyptian patients diagnosed with advanced hepatocelleular carcinoma. We observed an overall increase in miRNA expression in HCC samples compared with normal subjects. Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples. Comparing the pretreatment miRNA expression profiles in HCC patients and the patients response to TACE treatment resulted in the identification of a set of 12 miRNAs that are significantly upregulated in nonresponders group. This miRNA panel includes miR-10a-1, miR-23a-1, miR-24, miR-26a, miR-27a, miR-30c, miR-30e, miR-106b, miR-133b, miR-199a, miR-199-3p, and miR-200b. Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year. These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

No MeSH data available.


Related in: MedlinePlus

Comparison of miRNA profiles significantly deregulated in our study with published HCC studies. Boxplots of mean miRNA expression after control-based normalization are shown in all panels. Black bars indicate the median. *P < 0.05, **P < 0.01, and ***P < 0.001 are based on nonparametric Mann-Whitney tests comparing between miRNA expression levels in normal and tumours. (a) miRNA regulated in our study but not reported in other HCC studies. (b) miRNAs upregulated in our study and upregulated in other HCC studies. (c) miRNAs upregulated in our study and downregulated in other HCC studies.
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fig2: Comparison of miRNA profiles significantly deregulated in our study with published HCC studies. Boxplots of mean miRNA expression after control-based normalization are shown in all panels. Black bars indicate the median. *P < 0.05, **P < 0.01, and ***P < 0.001 are based on nonparametric Mann-Whitney tests comparing between miRNA expression levels in normal and tumours. (a) miRNA regulated in our study but not reported in other HCC studies. (b) miRNAs upregulated in our study and upregulated in other HCC studies. (c) miRNAs upregulated in our study and downregulated in other HCC studies.

Mentions: As far as we know, the current study is the first to profile miRNAs expression pattern in Egyptian HCC patients; we were interested to compare the expression profile of deregulated miRNA in our study with other published reports. In our study, a subset of miRNAs showed expression profiles comparable to the literature including miR-10a, miR-16, miR-21a, miR-24, miR-100, miR-106, miR-107, miR-122a, miR-155, miR-181b, miR-210, miR-221, miR-222, miR-324-5p, miR-491, and miR-151-3p (Figure 2(a)). Another subset of miRNAs was upregulated in our study but was downregulated in other studies including miR-26a, miR-30c, miR-30e, miR-99a, miR-125b, miR-145, miR-181a, miR-194, miR-199a-3p, miR-200a, miR-200b-3, miR-215, miR-223, miR-338, and miR-365 (Figure 2(b)). The expression pattern of the last subset in this group was not previously reported, including miR-23a, miR-31, miR-98, miR-106a, miR-133b, miR-154, miR-193b, miR-196b, miR-204, miR-326, miR-328, miR-455, miR-455-3p, miR-602, miR-664, and miR-1246 (Figure 2(c)).


Investigating the pretreatment miRNA expression patterns of advanced hepatocellular carcinoma patients in association with response to TACE treatment.

El-Halawany MS, Ismail HM, Zeeneldin AA, Elfiky A, Tantawy M, Kobaisi MH, Hamed I, Abdel Wahab AH - Biomed Res Int (2015)

Comparison of miRNA profiles significantly deregulated in our study with published HCC studies. Boxplots of mean miRNA expression after control-based normalization are shown in all panels. Black bars indicate the median. *P < 0.05, **P < 0.01, and ***P < 0.001 are based on nonparametric Mann-Whitney tests comparing between miRNA expression levels in normal and tumours. (a) miRNA regulated in our study but not reported in other HCC studies. (b) miRNAs upregulated in our study and upregulated in other HCC studies. (c) miRNAs upregulated in our study and downregulated in other HCC studies.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4355598&req=5

fig2: Comparison of miRNA profiles significantly deregulated in our study with published HCC studies. Boxplots of mean miRNA expression after control-based normalization are shown in all panels. Black bars indicate the median. *P < 0.05, **P < 0.01, and ***P < 0.001 are based on nonparametric Mann-Whitney tests comparing between miRNA expression levels in normal and tumours. (a) miRNA regulated in our study but not reported in other HCC studies. (b) miRNAs upregulated in our study and upregulated in other HCC studies. (c) miRNAs upregulated in our study and downregulated in other HCC studies.
Mentions: As far as we know, the current study is the first to profile miRNAs expression pattern in Egyptian HCC patients; we were interested to compare the expression profile of deregulated miRNA in our study with other published reports. In our study, a subset of miRNAs showed expression profiles comparable to the literature including miR-10a, miR-16, miR-21a, miR-24, miR-100, miR-106, miR-107, miR-122a, miR-155, miR-181b, miR-210, miR-221, miR-222, miR-324-5p, miR-491, and miR-151-3p (Figure 2(a)). Another subset of miRNAs was upregulated in our study but was downregulated in other studies including miR-26a, miR-30c, miR-30e, miR-99a, miR-125b, miR-145, miR-181a, miR-194, miR-199a-3p, miR-200a, miR-200b-3, miR-215, miR-223, miR-338, and miR-365 (Figure 2(b)). The expression pattern of the last subset in this group was not previously reported, including miR-23a, miR-31, miR-98, miR-106a, miR-133b, miR-154, miR-193b, miR-196b, miR-204, miR-326, miR-328, miR-455, miR-455-3p, miR-602, miR-664, and miR-1246 (Figure 2(c)).

Bottom Line: Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples.Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year.These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, Faculty of Science, Cairo University, El-Gamaa Street, Giza 12613, Egypt.

ABSTRACT
Hepatocellular carcinoma (HCC) is a lethal malignancy with poor prognosis and limited treatment options. Transarterial chemoembolization (TACE) using chemotherapy agents-doxorubicin and cisplatin-is an accepted treatment option for locally advanced hepatocellular carcinoma. In the current study, we analyzed the expression pattern of a selected panel of 94 miRNAs in archival samples that were collected prior to treatment from 15 Egyptian patients diagnosed with advanced hepatocelleular carcinoma. We observed an overall increase in miRNA expression in HCC samples compared with normal subjects. Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples. Comparing the pretreatment miRNA expression profiles in HCC patients and the patients response to TACE treatment resulted in the identification of a set of 12 miRNAs that are significantly upregulated in nonresponders group. This miRNA panel includes miR-10a-1, miR-23a-1, miR-24, miR-26a, miR-27a, miR-30c, miR-30e, miR-106b, miR-133b, miR-199a, miR-199-3p, and miR-200b. Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year. These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

No MeSH data available.


Related in: MedlinePlus