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Investigating the pretreatment miRNA expression patterns of advanced hepatocellular carcinoma patients in association with response to TACE treatment.

El-Halawany MS, Ismail HM, Zeeneldin AA, Elfiky A, Tantawy M, Kobaisi MH, Hamed I, Abdel Wahab AH - Biomed Res Int (2015)

Bottom Line: Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples.Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year.These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, Faculty of Science, Cairo University, El-Gamaa Street, Giza 12613, Egypt.

ABSTRACT
Hepatocellular carcinoma (HCC) is a lethal malignancy with poor prognosis and limited treatment options. Transarterial chemoembolization (TACE) using chemotherapy agents-doxorubicin and cisplatin-is an accepted treatment option for locally advanced hepatocellular carcinoma. In the current study, we analyzed the expression pattern of a selected panel of 94 miRNAs in archival samples that were collected prior to treatment from 15 Egyptian patients diagnosed with advanced hepatocelleular carcinoma. We observed an overall increase in miRNA expression in HCC samples compared with normal subjects. Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples. Comparing the pretreatment miRNA expression profiles in HCC patients and the patients response to TACE treatment resulted in the identification of a set of 12 miRNAs that are significantly upregulated in nonresponders group. This miRNA panel includes miR-10a-1, miR-23a-1, miR-24, miR-26a, miR-27a, miR-30c, miR-30e, miR-106b, miR-133b, miR-199a, miR-199-3p, and miR-200b. Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year. These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

No MeSH data available.


Related in: MedlinePlus

Profiling of 94 miRNA expression patterns in advanced HCC Egyptian patients compared to normal subjects. Expression of 94 miRNAs was determined by real-time PCR in specimens of normal controls (n = 10) and HCC patients (n = 15). (a) Heatmap of expression profile of 94 miRNAs in HCC patients (P1–P15) and normal donors (N1–N6). (b) Volcano plot of relative gene expression fold of change of the 93 miRNA versus P value. Fold of change is represented in log2 ratio in the x-axis versus P value represented in log10 ratio in the y-axis.
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fig1: Profiling of 94 miRNA expression patterns in advanced HCC Egyptian patients compared to normal subjects. Expression of 94 miRNAs was determined by real-time PCR in specimens of normal controls (n = 10) and HCC patients (n = 15). (a) Heatmap of expression profile of 94 miRNAs in HCC patients (P1–P15) and normal donors (N1–N6). (b) Volcano plot of relative gene expression fold of change of the 93 miRNA versus P value. Fold of change is represented in log2 ratio in the x-axis versus P value represented in log10 ratio in the y-axis.

Mentions: Expression profiles of 94 miRNAs were determined in paraffin tissues obtained from 15 HCC patients prior to TACE treatment with doxorubicin and cisplatin as well as a pool of 10 normal subjects. Of the 94 miRNAs analysed, 53 miRNAs were significantly upregulated (fold change: ≥3; P value < 0.05). Only three miRNAs were significantly downregulated (fold change: ≤3; P value < 0.05). The calculated values of fold of change in normal and tumour samples are summarized in Supplementary Table  1 (Supplementary Material is available online at http://dx.doi.org/10.1155/2015/649750). The log2 fold of change was used to generate expression profile heatmaps using GENE-E software. Figure 1(a) shows the heatmap for the 93 selected miRNAs. Figure 1(b) shows Volcano plot of fold of change (represented in log2 ratio in the x-axis) versus P value (represented in log10 ratio in the y-axis). Volcano plot shows a subset of miRNAs that are differentially deregulated in tumours compared to normal.


Investigating the pretreatment miRNA expression patterns of advanced hepatocellular carcinoma patients in association with response to TACE treatment.

El-Halawany MS, Ismail HM, Zeeneldin AA, Elfiky A, Tantawy M, Kobaisi MH, Hamed I, Abdel Wahab AH - Biomed Res Int (2015)

Profiling of 94 miRNA expression patterns in advanced HCC Egyptian patients compared to normal subjects. Expression of 94 miRNAs was determined by real-time PCR in specimens of normal controls (n = 10) and HCC patients (n = 15). (a) Heatmap of expression profile of 94 miRNAs in HCC patients (P1–P15) and normal donors (N1–N6). (b) Volcano plot of relative gene expression fold of change of the 93 miRNA versus P value. Fold of change is represented in log2 ratio in the x-axis versus P value represented in log10 ratio in the y-axis.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4355598&req=5

fig1: Profiling of 94 miRNA expression patterns in advanced HCC Egyptian patients compared to normal subjects. Expression of 94 miRNAs was determined by real-time PCR in specimens of normal controls (n = 10) and HCC patients (n = 15). (a) Heatmap of expression profile of 94 miRNAs in HCC patients (P1–P15) and normal donors (N1–N6). (b) Volcano plot of relative gene expression fold of change of the 93 miRNA versus P value. Fold of change is represented in log2 ratio in the x-axis versus P value represented in log10 ratio in the y-axis.
Mentions: Expression profiles of 94 miRNAs were determined in paraffin tissues obtained from 15 HCC patients prior to TACE treatment with doxorubicin and cisplatin as well as a pool of 10 normal subjects. Of the 94 miRNAs analysed, 53 miRNAs were significantly upregulated (fold change: ≥3; P value < 0.05). Only three miRNAs were significantly downregulated (fold change: ≤3; P value < 0.05). The calculated values of fold of change in normal and tumour samples are summarized in Supplementary Table  1 (Supplementary Material is available online at http://dx.doi.org/10.1155/2015/649750). The log2 fold of change was used to generate expression profile heatmaps using GENE-E software. Figure 1(a) shows the heatmap for the 93 selected miRNAs. Figure 1(b) shows Volcano plot of fold of change (represented in log2 ratio in the x-axis) versus P value (represented in log10 ratio in the y-axis). Volcano plot shows a subset of miRNAs that are differentially deregulated in tumours compared to normal.

Bottom Line: Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples.Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year.These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

View Article: PubMed Central - PubMed

Affiliation: Zoology Department, Faculty of Science, Cairo University, El-Gamaa Street, Giza 12613, Egypt.

ABSTRACT
Hepatocellular carcinoma (HCC) is a lethal malignancy with poor prognosis and limited treatment options. Transarterial chemoembolization (TACE) using chemotherapy agents-doxorubicin and cisplatin-is an accepted treatment option for locally advanced hepatocellular carcinoma. In the current study, we analyzed the expression pattern of a selected panel of 94 miRNAs in archival samples that were collected prior to treatment from 15 Egyptian patients diagnosed with advanced hepatocelleular carcinoma. We observed an overall increase in miRNA expression in HCC samples compared with normal subjects. Out of 94 examined miRNAs, 53 were significantly upregulated while 3 miRNAs were downregulated in HCC samples compared to normal liver samples. Comparing the pretreatment miRNA expression profiles in HCC patients and the patients response to TACE treatment resulted in the identification of a set of 12 miRNAs that are significantly upregulated in nonresponders group. This miRNA panel includes miR-10a-1, miR-23a-1, miR-24, miR-26a, miR-27a, miR-30c, miR-30e, miR-106b, miR-133b, miR-199a, miR-199-3p, and miR-200b. Furthermore, we observed that a panel of 10 miRNAs was significantly associated with patients' survival status at 1 year. These results highlight the potential implications of pretreatment miRNAs expression profiling in prediction of the patients' response to TACE treatment in liver cancer.

No MeSH data available.


Related in: MedlinePlus