Limits...
Are human peripheral nerves sensitive to X-ray imaging?

Scopel JF, de Souza Queiroz L, O'Dowd FP, Júnior MC, Nucci A, Hönnicke MG - PLoS ONE (2015)

Bottom Line: The outstanding result is the detection of such structures by phase contrast x-ray tomography of a thick human sciatic nerve section.This may further enable the identification of diverse pathological patterns, such as Wallerian degeneration, hypertrophic neuropathy, inflammatory infiltration, leprosy neuropathy and amyloid deposits.To the best of our knowledge, this is the first successful phase contrast x-ray imaging experiment of a human peripheral nerve sample.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Ciências da Saúde, Universidade Federal de Goiás, Jataí, Goiás, 75804-020, Brazil.

ABSTRACT
Diagnostic imaging techniques play an important role in assessing the exact location, cause, and extent of a nerve lesion, thus allowing clinicians to diagnose and manage more effectively a variety of pathological conditions, such as entrapment syndromes, traumatic injuries, and space-occupying lesions. Ultrasound and nuclear magnetic resonance imaging are becoming useful methods for this purpose, but they still lack spatial resolution. In this regard, recent phase contrast x-ray imaging experiments of peripheral nerve allowed the visualization of each nerve fiber surrounded by its myelin sheath as clearly as optical microscopy. In the present study, we attempted to produce high-resolution x-ray phase contrast images of a human sciatic nerve by using synchrotron radiation propagation-based imaging. The images showed high contrast and high spatial resolution, allowing clear identification of each fascicle structure and surrounding connective tissue. The outstanding result is the detection of such structures by phase contrast x-ray tomography of a thick human sciatic nerve section. This may further enable the identification of diverse pathological patterns, such as Wallerian degeneration, hypertrophic neuropathy, inflammatory infiltration, leprosy neuropathy and amyloid deposits. To the best of our knowledge, this is the first successful phase contrast x-ray imaging experiment of a human peripheral nerve sample. Our long-term goal is to develop peripheral nerve imaging methods that could supersede biopsy procedures.

Show MeSH

Related in: MedlinePlus

Axial slice of phase contrast x-ray tomography (a).Dose for the full tomography scan (1000 projections) was 0.23 mGy. Detailed axial section is shown in (b) and, for comparison, its corresponding optical microscope image in (c). Clear delineation of each nerve fascicle and surrounding structures are possible without sectioning, fixation or staining procedures with PC tomography. In the images, nerve fascicles (nf), an artery (arrow head), arterioles (thin arrow), venules (thick arrow), epineurium (square) and perineurium (ellipse) can be easily distinguished.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4355589&req=5

pone.0116831.g004: Axial slice of phase contrast x-ray tomography (a).Dose for the full tomography scan (1000 projections) was 0.23 mGy. Detailed axial section is shown in (b) and, for comparison, its corresponding optical microscope image in (c). Clear delineation of each nerve fascicle and surrounding structures are possible without sectioning, fixation or staining procedures with PC tomography. In the images, nerve fascicles (nf), an artery (arrow head), arterioles (thin arrow), venules (thick arrow), epineurium (square) and perineurium (ellipse) can be easily distinguished.

Mentions: Unprecedented phase contrast x-ray tomography results allowed us to delineate fine nerve structures, with almost the same resolution and contrast of the radiography, and without the need of sectioning, fixation or staining procedures (see Fig. 4). An artery is also visible inside the nerve tissue, with its exact counterpart seen in the optical microscopy image (see Fig. 4C). Note that the elements on the optical microscopy images are slightly loosened because of paraffin wax preparation artifacts.


Are human peripheral nerves sensitive to X-ray imaging?

Scopel JF, de Souza Queiroz L, O'Dowd FP, Júnior MC, Nucci A, Hönnicke MG - PLoS ONE (2015)

Axial slice of phase contrast x-ray tomography (a).Dose for the full tomography scan (1000 projections) was 0.23 mGy. Detailed axial section is shown in (b) and, for comparison, its corresponding optical microscope image in (c). Clear delineation of each nerve fascicle and surrounding structures are possible without sectioning, fixation or staining procedures with PC tomography. In the images, nerve fascicles (nf), an artery (arrow head), arterioles (thin arrow), venules (thick arrow), epineurium (square) and perineurium (ellipse) can be easily distinguished.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4355589&req=5

pone.0116831.g004: Axial slice of phase contrast x-ray tomography (a).Dose for the full tomography scan (1000 projections) was 0.23 mGy. Detailed axial section is shown in (b) and, for comparison, its corresponding optical microscope image in (c). Clear delineation of each nerve fascicle and surrounding structures are possible without sectioning, fixation or staining procedures with PC tomography. In the images, nerve fascicles (nf), an artery (arrow head), arterioles (thin arrow), venules (thick arrow), epineurium (square) and perineurium (ellipse) can be easily distinguished.
Mentions: Unprecedented phase contrast x-ray tomography results allowed us to delineate fine nerve structures, with almost the same resolution and contrast of the radiography, and without the need of sectioning, fixation or staining procedures (see Fig. 4). An artery is also visible inside the nerve tissue, with its exact counterpart seen in the optical microscopy image (see Fig. 4C). Note that the elements on the optical microscopy images are slightly loosened because of paraffin wax preparation artifacts.

Bottom Line: The outstanding result is the detection of such structures by phase contrast x-ray tomography of a thick human sciatic nerve section.This may further enable the identification of diverse pathological patterns, such as Wallerian degeneration, hypertrophic neuropathy, inflammatory infiltration, leprosy neuropathy and amyloid deposits.To the best of our knowledge, this is the first successful phase contrast x-ray imaging experiment of a human peripheral nerve sample.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Ciências da Saúde, Universidade Federal de Goiás, Jataí, Goiás, 75804-020, Brazil.

ABSTRACT
Diagnostic imaging techniques play an important role in assessing the exact location, cause, and extent of a nerve lesion, thus allowing clinicians to diagnose and manage more effectively a variety of pathological conditions, such as entrapment syndromes, traumatic injuries, and space-occupying lesions. Ultrasound and nuclear magnetic resonance imaging are becoming useful methods for this purpose, but they still lack spatial resolution. In this regard, recent phase contrast x-ray imaging experiments of peripheral nerve allowed the visualization of each nerve fiber surrounded by its myelin sheath as clearly as optical microscopy. In the present study, we attempted to produce high-resolution x-ray phase contrast images of a human sciatic nerve by using synchrotron radiation propagation-based imaging. The images showed high contrast and high spatial resolution, allowing clear identification of each fascicle structure and surrounding connective tissue. The outstanding result is the detection of such structures by phase contrast x-ray tomography of a thick human sciatic nerve section. This may further enable the identification of diverse pathological patterns, such as Wallerian degeneration, hypertrophic neuropathy, inflammatory infiltration, leprosy neuropathy and amyloid deposits. To the best of our knowledge, this is the first successful phase contrast x-ray imaging experiment of a human peripheral nerve sample. Our long-term goal is to develop peripheral nerve imaging methods that could supersede biopsy procedures.

Show MeSH
Related in: MedlinePlus