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Structural alterations of the intestinal epithelial barrier in Parkinson's disease.

Clairembault T, Leclair-Visonneau L, Coron E, Bourreille A, Le Dily S, Vavasseur F, Heymann MF, Neunlist M, Derkinderen P - Acta Neuropathol Commun (2015)

Bottom Line: The expression and localization of the two tight junctions proteins ZO-1 and occludin were analyzed by Western blot and immunofluorescence, respectively.The para- and transcellular permeability were not different between PD patients and controls.Our findings provide evidence that the IEB is morphologically altered in PD and further reinforce the potential role of the gastrointestinal tract in the initiation and/or the progression of the disease.

View Article: PubMed Central - PubMed

ABSTRACT
Functional and morphological alterations of the intestinal epithelial barrier (IEB) have been consistently reported in digestive disorders such as irritable bowel syndrome and inflammatory bowel disease. There is mounting evidence that Parkinson's disease (PD) is not only a brain disease but also a digestive disorder. Gastrointestinal involvement is a frequent and early event in the course of PD, and it may be critically involved in the early development of the disease. We therefore undertook the present survey to investigate whether changes in the IEB function and/or morphology occur in PD. Colonic biopsies were performed in 31 PD patients and 11 age-matched healthy controls. The para- and transcellular permeability were evaluated by measuring sulfonic acid and horseradish peroxidase flux respectively, in colonic biopsies mounted in Ussing chambers. The expression and localization of the two tight junctions proteins ZO-1 and occludin were analyzed by Western blot and immunofluorescence, respectively. The para- and transcellular permeability were not different between PD patients and controls. The expression of occludin, but not ZO-1, was significantly lower in colonic samples from PD patients as compared to controls and the cellular distribution of both proteins was altered in colonic mucosal specimens from PD patients. Our findings provide evidence that the IEB is morphologically altered in PD and further reinforce the potential role of the gastrointestinal tract in the initiation and/or the progression of the disease.

No MeSH data available.


Related in: MedlinePlus

Comparison of para- and transcellular permeability in PD patients and healthy controls. (A) For the evaluation of paracellular permeability, the flux of sulfonic acid (SA flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in arbitrary units (AU), in PD patients (n = 31) and controls (CTRL, n = 11). No significant changes were observed between the two groups (p = 0.65). (B) For the evaluation of paracellular permeability, the flux of horseradish peroxidase (HRP flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in ng/mL/min, in PD patients (n = 21) and controls (CTRL, n = 9). No significant changes were observed between the two groups (p = 0.39).
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Fig1: Comparison of para- and transcellular permeability in PD patients and healthy controls. (A) For the evaluation of paracellular permeability, the flux of sulfonic acid (SA flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in arbitrary units (AU), in PD patients (n = 31) and controls (CTRL, n = 11). No significant changes were observed between the two groups (p = 0.65). (B) For the evaluation of paracellular permeability, the flux of horseradish peroxidase (HRP flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in ng/mL/min, in PD patients (n = 21) and controls (CTRL, n = 9). No significant changes were observed between the two groups (p = 0.39).

Mentions: In a first set of experiments, we evaluated whether IEB is functionally altered in PD patients. The para- and transcellular permeability of colonic biopsies were measured in Ussing chambers in both PD patients and control subjects using sulfonic acid and HRP, respectively. No difference in the sulfonic acid flux was observed between PD patients and control subjects (n = 31 and 11, respectively; p = 0.65) (Figure 1A). HRP flux was also comparable between PD subjects and healthy controls (n = 21 and 9, respectively; p = 0.39) (Figure 1B). Although not statistically different from controls, the sulfonic acid and HRP flux values were heterogeneous between PD patients (Figure 1A and B). We thus investigated if the main clinical features of the disease had any influence on IEB permeability. We did not observe any correlation between age, disease duration or lifetime cumulative dose of L-DOPA and the values of sulfonic acid or HRP flux (Table 2).Figure 1


Structural alterations of the intestinal epithelial barrier in Parkinson's disease.

Clairembault T, Leclair-Visonneau L, Coron E, Bourreille A, Le Dily S, Vavasseur F, Heymann MF, Neunlist M, Derkinderen P - Acta Neuropathol Commun (2015)

Comparison of para- and transcellular permeability in PD patients and healthy controls. (A) For the evaluation of paracellular permeability, the flux of sulfonic acid (SA flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in arbitrary units (AU), in PD patients (n = 31) and controls (CTRL, n = 11). No significant changes were observed between the two groups (p = 0.65). (B) For the evaluation of paracellular permeability, the flux of horseradish peroxidase (HRP flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in ng/mL/min, in PD patients (n = 21) and controls (CTRL, n = 9). No significant changes were observed between the two groups (p = 0.39).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4353469&req=5

Fig1: Comparison of para- and transcellular permeability in PD patients and healthy controls. (A) For the evaluation of paracellular permeability, the flux of sulfonic acid (SA flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in arbitrary units (AU), in PD patients (n = 31) and controls (CTRL, n = 11). No significant changes were observed between the two groups (p = 0.65). (B) For the evaluation of paracellular permeability, the flux of horseradish peroxidase (HRP flux) was measured in colonic biopsies mounted in Ussing chambers, expressed in ng/mL/min, in PD patients (n = 21) and controls (CTRL, n = 9). No significant changes were observed between the two groups (p = 0.39).
Mentions: In a first set of experiments, we evaluated whether IEB is functionally altered in PD patients. The para- and transcellular permeability of colonic biopsies were measured in Ussing chambers in both PD patients and control subjects using sulfonic acid and HRP, respectively. No difference in the sulfonic acid flux was observed between PD patients and control subjects (n = 31 and 11, respectively; p = 0.65) (Figure 1A). HRP flux was also comparable between PD subjects and healthy controls (n = 21 and 9, respectively; p = 0.39) (Figure 1B). Although not statistically different from controls, the sulfonic acid and HRP flux values were heterogeneous between PD patients (Figure 1A and B). We thus investigated if the main clinical features of the disease had any influence on IEB permeability. We did not observe any correlation between age, disease duration or lifetime cumulative dose of L-DOPA and the values of sulfonic acid or HRP flux (Table 2).Figure 1

Bottom Line: The expression and localization of the two tight junctions proteins ZO-1 and occludin were analyzed by Western blot and immunofluorescence, respectively.The para- and transcellular permeability were not different between PD patients and controls.Our findings provide evidence that the IEB is morphologically altered in PD and further reinforce the potential role of the gastrointestinal tract in the initiation and/or the progression of the disease.

View Article: PubMed Central - PubMed

ABSTRACT
Functional and morphological alterations of the intestinal epithelial barrier (IEB) have been consistently reported in digestive disorders such as irritable bowel syndrome and inflammatory bowel disease. There is mounting evidence that Parkinson's disease (PD) is not only a brain disease but also a digestive disorder. Gastrointestinal involvement is a frequent and early event in the course of PD, and it may be critically involved in the early development of the disease. We therefore undertook the present survey to investigate whether changes in the IEB function and/or morphology occur in PD. Colonic biopsies were performed in 31 PD patients and 11 age-matched healthy controls. The para- and transcellular permeability were evaluated by measuring sulfonic acid and horseradish peroxidase flux respectively, in colonic biopsies mounted in Ussing chambers. The expression and localization of the two tight junctions proteins ZO-1 and occludin were analyzed by Western blot and immunofluorescence, respectively. The para- and transcellular permeability were not different between PD patients and controls. The expression of occludin, but not ZO-1, was significantly lower in colonic samples from PD patients as compared to controls and the cellular distribution of both proteins was altered in colonic mucosal specimens from PD patients. Our findings provide evidence that the IEB is morphologically altered in PD and further reinforce the potential role of the gastrointestinal tract in the initiation and/or the progression of the disease.

No MeSH data available.


Related in: MedlinePlus