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Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation.

Weng J, Liu Y, Xu Y, Hu R, Zhang H, Sheng X, Watanabe G, Taya K, Weng Q, Xu M - Int J Endocrinol (2015)

Bottom Line: The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period.The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period.Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.

ABSTRACT
One specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana dybowskii during the breeding period and prehibernation. The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period. P450arom was observed in stromal tissue in both the breeding period and prehibernation. ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period. These results suggested that estrogen may play autocrine/paracrine roles mediated by ERα in regulating the oviductal hypertrophy during prehibernation.

No MeSH data available.


Related in: MedlinePlus

The relative CYP19 (a) and ERα (b) mRNA in the oviduct of Rana dybowskii during the breeding period (B) and prehibernation (P). Bars represent mean ± SD for three independent experiments. Mean values within the columns marked the same as above indicate significant difference (P < 0.05). Compared with Rana rugosa (Rr), the cDNA nucleotide sequence identity was 91.43% for Cyp19 (c) and 94.15% for ERα (d).
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fig4: The relative CYP19 (a) and ERα (b) mRNA in the oviduct of Rana dybowskii during the breeding period (B) and prehibernation (P). Bars represent mean ± SD for three independent experiments. Mean values within the columns marked the same as above indicate significant difference (P < 0.05). Compared with Rana rugosa (Rr), the cDNA nucleotide sequence identity was 91.43% for Cyp19 (c) and 94.15% for ERα (d).

Mentions: Cyp19 (Figure 4(a)) and ERα (Figure 4(b)) mRNA levels were detected in oviductal tissues of Rana dybowskii during the breeding period and prehibernation. The primers specific for Cyp19 was used to amplify a Real-Time PCR product of 304 bp, while production of 180 bp for ERα. Meanwhile, the mRNA expressions of these two genes were generally in line with those of their corresponding proteins. Real-time PCR analysis revealed a significant increase in Cyp19 and ERαmRNA levels during prehibernation as compared to the breeding period. ERβgene was not expressed in oviductal tissues of Rana dybowskii in the breeding period and prehibernation (data not shown). To further confirm the nature of the signals, cDNA fragments for Cyp19 and ERαin oviductal tissues of Rana dybowskii were sequenced and compared to the corresponding fragments in Rana rugosa. The 304 bp Cyp19 cDNA nucleotide sequence identity is 94.15% (Figure 4(c)) and the 180 bp ERα cDNA nucleotide sequence identity is 91.43% (Figure 4(d)).


Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation.

Weng J, Liu Y, Xu Y, Hu R, Zhang H, Sheng X, Watanabe G, Taya K, Weng Q, Xu M - Int J Endocrinol (2015)

The relative CYP19 (a) and ERα (b) mRNA in the oviduct of Rana dybowskii during the breeding period (B) and prehibernation (P). Bars represent mean ± SD for three independent experiments. Mean values within the columns marked the same as above indicate significant difference (P < 0.05). Compared with Rana rugosa (Rr), the cDNA nucleotide sequence identity was 91.43% for Cyp19 (c) and 94.15% for ERα (d).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4353437&req=5

fig4: The relative CYP19 (a) and ERα (b) mRNA in the oviduct of Rana dybowskii during the breeding period (B) and prehibernation (P). Bars represent mean ± SD for three independent experiments. Mean values within the columns marked the same as above indicate significant difference (P < 0.05). Compared with Rana rugosa (Rr), the cDNA nucleotide sequence identity was 91.43% for Cyp19 (c) and 94.15% for ERα (d).
Mentions: Cyp19 (Figure 4(a)) and ERα (Figure 4(b)) mRNA levels were detected in oviductal tissues of Rana dybowskii during the breeding period and prehibernation. The primers specific for Cyp19 was used to amplify a Real-Time PCR product of 304 bp, while production of 180 bp for ERα. Meanwhile, the mRNA expressions of these two genes were generally in line with those of their corresponding proteins. Real-time PCR analysis revealed a significant increase in Cyp19 and ERαmRNA levels during prehibernation as compared to the breeding period. ERβgene was not expressed in oviductal tissues of Rana dybowskii in the breeding period and prehibernation (data not shown). To further confirm the nature of the signals, cDNA fragments for Cyp19 and ERαin oviductal tissues of Rana dybowskii were sequenced and compared to the corresponding fragments in Rana rugosa. The 304 bp Cyp19 cDNA nucleotide sequence identity is 94.15% (Figure 4(c)) and the 180 bp ERα cDNA nucleotide sequence identity is 91.43% (Figure 4(d)).

Bottom Line: The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period.The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period.Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.

ABSTRACT
One specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana dybowskii during the breeding period and prehibernation. The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period. P450arom was observed in stromal tissue in both the breeding period and prehibernation. ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period. These results suggested that estrogen may play autocrine/paracrine roles mediated by ERα in regulating the oviductal hypertrophy during prehibernation.

No MeSH data available.


Related in: MedlinePlus