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Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation.

Weng J, Liu Y, Xu Y, Hu R, Zhang H, Sheng X, Watanabe G, Taya K, Weng Q, Xu M - Int J Endocrinol (2015)

Bottom Line: The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period.The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period.Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.

ABSTRACT
One specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana dybowskii during the breeding period and prehibernation. The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period. P450arom was observed in stromal tissue in both the breeding period and prehibernation. ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period. These results suggested that estrogen may play autocrine/paracrine roles mediated by ERα in regulating the oviductal hypertrophy during prehibernation.

No MeSH data available.


Related in: MedlinePlus

Changes in oviductal histology between the breeding period and prehibernation. Anatomic localization and morphology of oviduct of Rana dybowskii during the breeding period (a) and prehibernation (c). Histological structure of Rana dybowskii oviducts by hematoxylin and eosin staining in the breeding period (b) and prehibernation (d). Tubule lumen, epithelial cells, and lobules consist of stroma cells and were observed in oviduct tissues expanded. Changes in weight (e) and relative circumference (f) of oviduct in Rana dybowskii from the breeding period to prehibernation. B: the breeding period; P: prehibernation; EC: epithelial cell; ST: stroma tissue; TL: tubule lumen. Scale bars represent 1 cm ((a), (c)) and 0.2 mm ((b), (d)), respectively.
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fig1: Changes in oviductal histology between the breeding period and prehibernation. Anatomic localization and morphology of oviduct of Rana dybowskii during the breeding period (a) and prehibernation (c). Histological structure of Rana dybowskii oviducts by hematoxylin and eosin staining in the breeding period (b) and prehibernation (d). Tubule lumen, epithelial cells, and lobules consist of stroma cells and were observed in oviduct tissues expanded. Changes in weight (e) and relative circumference (f) of oviduct in Rana dybowskii from the breeding period to prehibernation. B: the breeding period; P: prehibernation; EC: epithelial cell; ST: stroma tissue; TL: tubule lumen. Scale bars represent 1 cm ((a), (c)) and 0.2 mm ((b), (d)), respectively.

Mentions: Anatomic and morphologic observations of oviduct were present in the female Rana dybowskii during the breeding period and prehibernation (Figures 1(a) and 1(c)). The histological appearances of oviductal tissues from the breeding period and prehibernation were shown in Figures 1(b) and 1(d). The oviduct included tubule lumen, epithelium, and lobules that consist of stromal cells. The higher oviductal weight and relative circumference values of the oviduct were observed during prehibernation in October and lower values were found during breeding period in April (Figures 1(e) and 1(f)).


Expression of P450arom and Estrogen Receptor Alpha in the Oviduct of Chinese Brown Frog (Rana dybowskii) during Prehibernation.

Weng J, Liu Y, Xu Y, Hu R, Zhang H, Sheng X, Watanabe G, Taya K, Weng Q, Xu M - Int J Endocrinol (2015)

Changes in oviductal histology between the breeding period and prehibernation. Anatomic localization and morphology of oviduct of Rana dybowskii during the breeding period (a) and prehibernation (c). Histological structure of Rana dybowskii oviducts by hematoxylin and eosin staining in the breeding period (b) and prehibernation (d). Tubule lumen, epithelial cells, and lobules consist of stroma cells and were observed in oviduct tissues expanded. Changes in weight (e) and relative circumference (f) of oviduct in Rana dybowskii from the breeding period to prehibernation. B: the breeding period; P: prehibernation; EC: epithelial cell; ST: stroma tissue; TL: tubule lumen. Scale bars represent 1 cm ((a), (c)) and 0.2 mm ((b), (d)), respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4353437&req=5

fig1: Changes in oviductal histology between the breeding period and prehibernation. Anatomic localization and morphology of oviduct of Rana dybowskii during the breeding period (a) and prehibernation (c). Histological structure of Rana dybowskii oviducts by hematoxylin and eosin staining in the breeding period (b) and prehibernation (d). Tubule lumen, epithelial cells, and lobules consist of stroma cells and were observed in oviduct tissues expanded. Changes in weight (e) and relative circumference (f) of oviduct in Rana dybowskii from the breeding period to prehibernation. B: the breeding period; P: prehibernation; EC: epithelial cell; ST: stroma tissue; TL: tubule lumen. Scale bars represent 1 cm ((a), (c)) and 0.2 mm ((b), (d)), respectively.
Mentions: Anatomic and morphologic observations of oviduct were present in the female Rana dybowskii during the breeding period and prehibernation (Figures 1(a) and 1(c)). The histological appearances of oviductal tissues from the breeding period and prehibernation were shown in Figures 1(b) and 1(d). The oviduct included tubule lumen, epithelium, and lobules that consist of stromal cells. The higher oviductal weight and relative circumference values of the oviduct were observed during prehibernation in October and lower values were found during breeding period in April (Figures 1(e) and 1(f)).

Bottom Line: The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period.The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period.Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period.

View Article: PubMed Central - PubMed

Affiliation: College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China.

ABSTRACT
One specific physiological phenomenon of Chinese brown frog (Rana dybowskii) is that its oviduct expands prior to hibernation instead of expanding during the breeding period. In this study, we investigated the expression of P450arom and estrogen receptors α and β (ERα and ERβ) in the oviduct of Rana dybowskii during the breeding period and prehibernation. The results of the present study showed that there were significant differences in both oviductal weight and size with values markedly higher in prehibernation than in the breeding period. P450arom was observed in stromal tissue in both the breeding period and prehibernation. ERα was expressed in stromal tissue and epithelial cells in both periods, whereas ERβ could not be detected. The mean protein and mRNA levels of P450arom and ERα were significantly higher in prehibernation as compared to the breeding period. Besides, oviductal content of 17β-estradiol was also higher in prehibernation than in the breeding period. These results suggested that estrogen may play autocrine/paracrine roles mediated by ERα in regulating the oviductal hypertrophy during prehibernation.

No MeSH data available.


Related in: MedlinePlus