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Reversible energy-transfer switching on a DNA scaffold.

Bälter M, Hammarson M, Remón P, Li S, Gale N, Brown T, Andréasson J - J. Am. Chem. Soc. (2015)

Bottom Line: We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative.UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state.The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology , 412 96 Göteborg, Sweden.

ABSTRACT
We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

No MeSH data available.


Related in: MedlinePlus

IsomerizationScheme for Spiropyran Derivative 1 Usedin This Study
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Reversible energy-transfer switching on a DNA scaffold.

Bälter M, Hammarson M, Remón P, Li S, Gale N, Brown T, Andréasson J - J. Am. Chem. Soc. (2015)

IsomerizationScheme for Spiropyran Derivative 1 Usedin This Study
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4353014&req=5

sch1: IsomerizationScheme for Spiropyran Derivative 1 Usedin This Study
Bottom Line: We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative.UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state.The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology , 412 96 Göteborg, Sweden.

ABSTRACT
We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

No MeSH data available.


Related in: MedlinePlus