A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella.
Bottom Line: Yeast two-hybrid studies revealed that meiosis-expressed gene 1 (MEIG1) and Parkin co-regulated gene (PACRG) interact, and that sperm-associated antigen 16, which encodes an axoneme central apparatus protein, is also a binding partner of MEIG1.PACRG is not stable in mammalian cells, but can be stabilized by MEIG1 or by inhibition of proteasome function.Together, our studies demonstrate that MEIG1/PACRG forms a complex in the manchette and that this complex is necessary to transport cargos, such as SPAG16L, to build the sperm flagella.
Affiliation: Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA 23298, USA.Show MeSH
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Mentions: In adult Meig1-deficient mice, spermatogenesis is arrested in spermiogenesis (Zhang et al., 2009). To further examine when the testes of Meig1-deficient mice undergo pathological changes during the first wave of spermatogenesis, testes from wild-type and Meig1-deficient mice were collected at different ages, from day 17 to day 35 after birth, and tissue sections were stained with H&E. Testicular histology in Meig1-deficient mice remains normal until 28 days after birth, but after this time point, when spermatids enter the condensation/elongation stage, they cease differentiation (Fig. 1).Fig. 1.
Affiliation: Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA 23298, USA.