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A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella.

Li W, Tang W, Teves ME, Zhang Z, Zhang L, Li H, Archer KJ, Peterson DL, Williams DC, Strauss JF, Zhang Z - Development (2015)

Bottom Line: Yeast two-hybrid studies revealed that meiosis-expressed gene 1 (MEIG1) and Parkin co-regulated gene (PACRG) interact, and that sperm-associated antigen 16, which encodes an axoneme central apparatus protein, is also a binding partner of MEIG1.PACRG is not stable in mammalian cells, but can be stabilized by MEIG1 or by inhibition of proteasome function.Together, our studies demonstrate that MEIG1/PACRG forms a complex in the manchette and that this complex is necessary to transport cargos, such as SPAG16L, to build the sperm flagella.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA 23298, USA.

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Dynamic analysis of testicular histology of Meig1-deficient mice during the first wave of spermatogenesis. Representative testicular sections stained with Hematoxylin and Eosin from wild-type (WT) and Meig1-deficient (KO) mice at the indicated ages. Testicular histology in Meig1-deficient mice is still normal 28 days after birth, but after this time point, when spermatids enter the condensation/elongation stage, they cease differentiation. The white dashed arrows indicate the released germ cells in the lumen of seminiferous tubules of wild-type mice at day 30 and 35 after birth, the white arrows indicate an empty lumen of the seminiferous tubules of Meig1-deficient mice at the same age.
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DEV119834F1: Dynamic analysis of testicular histology of Meig1-deficient mice during the first wave of spermatogenesis. Representative testicular sections stained with Hematoxylin and Eosin from wild-type (WT) and Meig1-deficient (KO) mice at the indicated ages. Testicular histology in Meig1-deficient mice is still normal 28 days after birth, but after this time point, when spermatids enter the condensation/elongation stage, they cease differentiation. The white dashed arrows indicate the released germ cells in the lumen of seminiferous tubules of wild-type mice at day 30 and 35 after birth, the white arrows indicate an empty lumen of the seminiferous tubules of Meig1-deficient mice at the same age.

Mentions: In adult Meig1-deficient mice, spermatogenesis is arrested in spermiogenesis (Zhang et al., 2009). To further examine when the testes of Meig1-deficient mice undergo pathological changes during the first wave of spermatogenesis, testes from wild-type and Meig1-deficient mice were collected at different ages, from day 17 to day 35 after birth, and tissue sections were stained with H&E. Testicular histology in Meig1-deficient mice remains normal until 28 days after birth, but after this time point, when spermatids enter the condensation/elongation stage, they cease differentiation (Fig. 1).Fig. 1.


A MEIG1/PACRG complex in the manchette is essential for building the sperm flagella.

Li W, Tang W, Teves ME, Zhang Z, Zhang L, Li H, Archer KJ, Peterson DL, Williams DC, Strauss JF, Zhang Z - Development (2015)

Dynamic analysis of testicular histology of Meig1-deficient mice during the first wave of spermatogenesis. Representative testicular sections stained with Hematoxylin and Eosin from wild-type (WT) and Meig1-deficient (KO) mice at the indicated ages. Testicular histology in Meig1-deficient mice is still normal 28 days after birth, but after this time point, when spermatids enter the condensation/elongation stage, they cease differentiation. The white dashed arrows indicate the released germ cells in the lumen of seminiferous tubules of wild-type mice at day 30 and 35 after birth, the white arrows indicate an empty lumen of the seminiferous tubules of Meig1-deficient mice at the same age.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352978&req=5

DEV119834F1: Dynamic analysis of testicular histology of Meig1-deficient mice during the first wave of spermatogenesis. Representative testicular sections stained with Hematoxylin and Eosin from wild-type (WT) and Meig1-deficient (KO) mice at the indicated ages. Testicular histology in Meig1-deficient mice is still normal 28 days after birth, but after this time point, when spermatids enter the condensation/elongation stage, they cease differentiation. The white dashed arrows indicate the released germ cells in the lumen of seminiferous tubules of wild-type mice at day 30 and 35 after birth, the white arrows indicate an empty lumen of the seminiferous tubules of Meig1-deficient mice at the same age.
Mentions: In adult Meig1-deficient mice, spermatogenesis is arrested in spermiogenesis (Zhang et al., 2009). To further examine when the testes of Meig1-deficient mice undergo pathological changes during the first wave of spermatogenesis, testes from wild-type and Meig1-deficient mice were collected at different ages, from day 17 to day 35 after birth, and tissue sections were stained with H&E. Testicular histology in Meig1-deficient mice remains normal until 28 days after birth, but after this time point, when spermatids enter the condensation/elongation stage, they cease differentiation (Fig. 1).Fig. 1.

Bottom Line: Yeast two-hybrid studies revealed that meiosis-expressed gene 1 (MEIG1) and Parkin co-regulated gene (PACRG) interact, and that sperm-associated antigen 16, which encodes an axoneme central apparatus protein, is also a binding partner of MEIG1.PACRG is not stable in mammalian cells, but can be stabilized by MEIG1 or by inhibition of proteasome function.Together, our studies demonstrate that MEIG1/PACRG forms a complex in the manchette and that this complex is necessary to transport cargos, such as SPAG16L, to build the sperm flagella.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA 23298, USA.

Show MeSH
Related in: MedlinePlus