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In Vitro Antistaphylococcal Effects of Embelia schimperi Extracts and Their Component Embelin with Oxacillin and Tetracycline.

Rondevaldova J, Leuner O, Teka A, Lulekal E, Havlik J, Van Damme P, Kokoska L - Evid Based Complement Alternat Med (2015)

Bottom Line: In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains.According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin.These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, Suchdol, 165 21 Prague 6, Czech Republic.

ABSTRACT
Bacterial infections are in less-developed countries traditionally treated by remedies prepared from medicinal plants. Embelia schimperi (Vatke) is a plant used as a taenicide or disinfectant in Ethiopia, very often taken mixed with another plant species. In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains. Minimum inhibitory concentrations were determined through the broth microdilution method, whereas the combinatory effect was evaluated through fractional inhibitory concentration sum (ΣFIC) indices. Results show many positive interactions and synergy occurring in embelin and oxacillin combinations against 4 out of 9 strains (ΣFIC 0.203-0.477) and for embelin and tetracycline combination against 3 out of 9 strains (ΣFIC 0.400-0.496). Moreover, the resistance to oxacillin has been overcome in 2 strains and to tetracycline in 3 strains. According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin. These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

No MeSH data available.


Related in: MedlinePlus

UV spectra of embelin as standard (a) and in extracts from twigs with leaves (b) and seeds (c).
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fig3: UV spectra of embelin as standard (a) and in extracts from twigs with leaves (b) and seeds (c).

Mentions: HPLC analysis identified embelin as the main secondary metabolite present in both extracts (Figures 2 and 3). The total content of embelin was 3022.6 μg/mL in extract 2 and 1476.5 μg/mL in extract 1, with a standard deviation of 89.1 and 40.7, corresponding to the content of 1.11% in twigs with leaves and 1.51% in seeds. From the chromatogram profiles, embelin was clearly the most intense peak at 260 nm. These results are consistent with those of Midiwo and Manguro [19] who described presence of embelin in E. schimperi and determined its content in fruits, root bark, stem bark, and leaves at relative ratio 1.01–4.31%.


In Vitro Antistaphylococcal Effects of Embelia schimperi Extracts and Their Component Embelin with Oxacillin and Tetracycline.

Rondevaldova J, Leuner O, Teka A, Lulekal E, Havlik J, Van Damme P, Kokoska L - Evid Based Complement Alternat Med (2015)

UV spectra of embelin as standard (a) and in extracts from twigs with leaves (b) and seeds (c).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352930&req=5

fig3: UV spectra of embelin as standard (a) and in extracts from twigs with leaves (b) and seeds (c).
Mentions: HPLC analysis identified embelin as the main secondary metabolite present in both extracts (Figures 2 and 3). The total content of embelin was 3022.6 μg/mL in extract 2 and 1476.5 μg/mL in extract 1, with a standard deviation of 89.1 and 40.7, corresponding to the content of 1.11% in twigs with leaves and 1.51% in seeds. From the chromatogram profiles, embelin was clearly the most intense peak at 260 nm. These results are consistent with those of Midiwo and Manguro [19] who described presence of embelin in E. schimperi and determined its content in fruits, root bark, stem bark, and leaves at relative ratio 1.01–4.31%.

Bottom Line: In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains.According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin.These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, Suchdol, 165 21 Prague 6, Czech Republic.

ABSTRACT
Bacterial infections are in less-developed countries traditionally treated by remedies prepared from medicinal plants. Embelia schimperi (Vatke) is a plant used as a taenicide or disinfectant in Ethiopia, very often taken mixed with another plant species. In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains. Minimum inhibitory concentrations were determined through the broth microdilution method, whereas the combinatory effect was evaluated through fractional inhibitory concentration sum (ΣFIC) indices. Results show many positive interactions and synergy occurring in embelin and oxacillin combinations against 4 out of 9 strains (ΣFIC 0.203-0.477) and for embelin and tetracycline combination against 3 out of 9 strains (ΣFIC 0.400-0.496). Moreover, the resistance to oxacillin has been overcome in 2 strains and to tetracycline in 3 strains. According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin. These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

No MeSH data available.


Related in: MedlinePlus