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In Vitro Antistaphylococcal Effects of Embelia schimperi Extracts and Their Component Embelin with Oxacillin and Tetracycline.

Rondevaldova J, Leuner O, Teka A, Lulekal E, Havlik J, Van Damme P, Kokoska L - Evid Based Complement Alternat Med (2015)

Bottom Line: In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains.According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin.These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, Suchdol, 165 21 Prague 6, Czech Republic.

ABSTRACT
Bacterial infections are in less-developed countries traditionally treated by remedies prepared from medicinal plants. Embelia schimperi (Vatke) is a plant used as a taenicide or disinfectant in Ethiopia, very often taken mixed with another plant species. In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains. Minimum inhibitory concentrations were determined through the broth microdilution method, whereas the combinatory effect was evaluated through fractional inhibitory concentration sum (ΣFIC) indices. Results show many positive interactions and synergy occurring in embelin and oxacillin combinations against 4 out of 9 strains (ΣFIC 0.203-0.477) and for embelin and tetracycline combination against 3 out of 9 strains (ΣFIC 0.400-0.496). Moreover, the resistance to oxacillin has been overcome in 2 strains and to tetracycline in 3 strains. According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin. These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

No MeSH data available.


Related in: MedlinePlus

HPLC chromatograms of standard embelin (a) and Embelia schimperi ethanol extracts from seeds (b) and from twigs with leaves (c) at 260 nm.
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fig2: HPLC chromatograms of standard embelin (a) and Embelia schimperi ethanol extracts from seeds (b) and from twigs with leaves (c) at 260 nm.

Mentions: For the determination of embelin, following concentrations were used: 51.2 mg/mL and 25.6 mg/mL of plant extracts and 0.5 mg/mL and 1 mg/mL of standard embelin. Subsequently, series of standard embelin solutions of concentrations 25, 32.5, 50, 75, 100, 150, 200, and 300 μg/mL were analyzed to create an external calibration curve and triplicate injection of 2.56 mg/mL of both extracts was performed to enable the quantification of embelin (Figures 1 and 2). Both determination and quantification were based on triplicate sample preparation. Limit of detection (LOD) and limit of quantification (LOQ) were estimated from the signal-to-noise ratio and were found to be 3.81 and 6.35 μg/mL, respectively.


In Vitro Antistaphylococcal Effects of Embelia schimperi Extracts and Their Component Embelin with Oxacillin and Tetracycline.

Rondevaldova J, Leuner O, Teka A, Lulekal E, Havlik J, Van Damme P, Kokoska L - Evid Based Complement Alternat Med (2015)

HPLC chromatograms of standard embelin (a) and Embelia schimperi ethanol extracts from seeds (b) and from twigs with leaves (c) at 260 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352930&req=5

fig2: HPLC chromatograms of standard embelin (a) and Embelia schimperi ethanol extracts from seeds (b) and from twigs with leaves (c) at 260 nm.
Mentions: For the determination of embelin, following concentrations were used: 51.2 mg/mL and 25.6 mg/mL of plant extracts and 0.5 mg/mL and 1 mg/mL of standard embelin. Subsequently, series of standard embelin solutions of concentrations 25, 32.5, 50, 75, 100, 150, 200, and 300 μg/mL were analyzed to create an external calibration curve and triplicate injection of 2.56 mg/mL of both extracts was performed to enable the quantification of embelin (Figures 1 and 2). Both determination and quantification were based on triplicate sample preparation. Limit of detection (LOD) and limit of quantification (LOQ) were estimated from the signal-to-noise ratio and were found to be 3.81 and 6.35 μg/mL, respectively.

Bottom Line: In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains.According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin.These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, Suchdol, 165 21 Prague 6, Czech Republic.

ABSTRACT
Bacterial infections are in less-developed countries traditionally treated by remedies prepared from medicinal plants. Embelia schimperi (Vatke) is a plant used as a taenicide or disinfectant in Ethiopia, very often taken mixed with another plant species. In the present study, we examined two extracts prepared from seeds and twigs with leaves of E. schimperi and its main present secondary metabolite embelin for their antibacterial combinatory effect with oxacillin and tetracycline against sensitive and resistant Staphylococcus aureus strains. Minimum inhibitory concentrations were determined through the broth microdilution method, whereas the combinatory effect was evaluated through fractional inhibitory concentration sum (ΣFIC) indices. Results show many positive interactions and synergy occurring in embelin and oxacillin combinations against 4 out of 9 strains (ΣFIC 0.203-0.477) and for embelin and tetracycline combination against 3 out of 9 strains (ΣFIC 0.400-0.496). Moreover, the resistance to oxacillin has been overcome in 2 strains and to tetracycline in 3 strains. According to our knowledge, this is the first study showing antimicrobial combinatory effect of E. schimperi as well as of embelin. These findings can be used for the further research targeted on the development of new antistaphylococcal agents.

No MeSH data available.


Related in: MedlinePlus