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Ejaculate oxidative stress is related with sperm DNA fragmentation and round cells.

Iommiello VM, Albani E, Di Rosa A, Marras A, Menduni F, Morreale G, Levi SL, Pisano B, Levi-Setti PE - Int J Endocrinol (2015)

Bottom Line: Our data show that high oxidative stress (N3-N4 levels) correlated positively with a DFI ≥ 30% (P = 0.0379) and round cells ≥1.500.000/mL (P = 0.0084).In conclusion, OS increases sperm DNA damage.Thus evaluation of semen OS extent of sperm DNA damage in infertile man could be useful to develop new therapeutic strategies and improve success of assisted reproduction techniques (ART).

View Article: PubMed Central - PubMed

Affiliation: Humanitas Fertility Center, Department of Gynaecology, Division of Gynecology and Reproductive Medicine, Humanitas Research Hospital, Via Manzoni 56, Rozzano, 20089 Milan, Italy.

ABSTRACT
Oxidative stress (OS) plays an essential role in male infertility aetiology by affecting sperm quality, function, and also the integrity of sperm DNA. The assessment of oxidative stress in semen may be an important tool to improve the evaluation of sperm reproductive capacity. The purpose of this study was the evaluation of any possible relation between the unbalance of oxidative stress caused by superoxide anion in the ejaculate with the presence of sperm DNA fragmentation and high concentration of round cells. 56 semen samples from males from couples suffering from infertility were evaluated according to World Health Organisation (WHO) 2010 guidelines. Oxidative stress levels from N1 (low) to N4 (high) were assessed in ejaculates using oxiSperm; DFI (sperm DNA fragmentation index) as assessed by the SCSA (Sperm Chromatin Structure Assay) was used for evaluation of sperm chromatin integrity. Our data show that high oxidative stress (N3-N4 levels) correlated positively with a DFI ≥ 30% (P = 0.0379) and round cells ≥1.500.000/mL (P = 0.0084). In conclusion, OS increases sperm DNA damage. Thus evaluation of semen OS extent of sperm DNA damage in infertile man could be useful to develop new therapeutic strategies and improve success of assisted reproduction techniques (ART).

No MeSH data available.


Related in: MedlinePlus

Scatter plots of SCSA analysis of a sperm sample with DFI ≥ 30% (a) and DFI < 30% (b) showing the ratio of green (not fragmented) and red (fragmented) sperm. The percentage of sperm with denatured DNA is reported as Comp alpha t (cells outside the main population).
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fig2: Scatter plots of SCSA analysis of a sperm sample with DFI ≥ 30% (a) and DFI < 30% (b) showing the ratio of green (not fragmented) and red (fragmented) sperm. The percentage of sperm with denatured DNA is reported as Comp alpha t (cells outside the main population).

Mentions: The percentage of sperms with denatured DNA was expressed in terms of DFI using FCS4 Express software that creates a scatter plot showing the ratio of green (not fragmented) and red (fragmented) sperms (Figure 2). A statistical threshold has been established to <30% and ≥30% DFI for normal and high sperm DNA fragmentation, respectively.


Ejaculate oxidative stress is related with sperm DNA fragmentation and round cells.

Iommiello VM, Albani E, Di Rosa A, Marras A, Menduni F, Morreale G, Levi SL, Pisano B, Levi-Setti PE - Int J Endocrinol (2015)

Scatter plots of SCSA analysis of a sperm sample with DFI ≥ 30% (a) and DFI < 30% (b) showing the ratio of green (not fragmented) and red (fragmented) sperm. The percentage of sperm with denatured DNA is reported as Comp alpha t (cells outside the main population).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352927&req=5

fig2: Scatter plots of SCSA analysis of a sperm sample with DFI ≥ 30% (a) and DFI < 30% (b) showing the ratio of green (not fragmented) and red (fragmented) sperm. The percentage of sperm with denatured DNA is reported as Comp alpha t (cells outside the main population).
Mentions: The percentage of sperms with denatured DNA was expressed in terms of DFI using FCS4 Express software that creates a scatter plot showing the ratio of green (not fragmented) and red (fragmented) sperms (Figure 2). A statistical threshold has been established to <30% and ≥30% DFI for normal and high sperm DNA fragmentation, respectively.

Bottom Line: Our data show that high oxidative stress (N3-N4 levels) correlated positively with a DFI ≥ 30% (P = 0.0379) and round cells ≥1.500.000/mL (P = 0.0084).In conclusion, OS increases sperm DNA damage.Thus evaluation of semen OS extent of sperm DNA damage in infertile man could be useful to develop new therapeutic strategies and improve success of assisted reproduction techniques (ART).

View Article: PubMed Central - PubMed

Affiliation: Humanitas Fertility Center, Department of Gynaecology, Division of Gynecology and Reproductive Medicine, Humanitas Research Hospital, Via Manzoni 56, Rozzano, 20089 Milan, Italy.

ABSTRACT
Oxidative stress (OS) plays an essential role in male infertility aetiology by affecting sperm quality, function, and also the integrity of sperm DNA. The assessment of oxidative stress in semen may be an important tool to improve the evaluation of sperm reproductive capacity. The purpose of this study was the evaluation of any possible relation between the unbalance of oxidative stress caused by superoxide anion in the ejaculate with the presence of sperm DNA fragmentation and high concentration of round cells. 56 semen samples from males from couples suffering from infertility were evaluated according to World Health Organisation (WHO) 2010 guidelines. Oxidative stress levels from N1 (low) to N4 (high) were assessed in ejaculates using oxiSperm; DFI (sperm DNA fragmentation index) as assessed by the SCSA (Sperm Chromatin Structure Assay) was used for evaluation of sperm chromatin integrity. Our data show that high oxidative stress (N3-N4 levels) correlated positively with a DFI ≥ 30% (P = 0.0379) and round cells ≥1.500.000/mL (P = 0.0084). In conclusion, OS increases sperm DNA damage. Thus evaluation of semen OS extent of sperm DNA damage in infertile man could be useful to develop new therapeutic strategies and improve success of assisted reproduction techniques (ART).

No MeSH data available.


Related in: MedlinePlus