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New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing.

Helguera M, Rivarola M, Clavijo B, Martis MM, Vanzetti LS, González S, Garbus I, Leroy P, Šimková H, Valárik M, Caccamo M, Doležel J, Mayer KF, Feuillet C, Tranquilli G, Paniego N, Echenique V - Plant Sci. (2014)

Bottom Line: The virtual order showed a higher collinearity with homeologous 4B compared to 4A.Additionally, a virtual map was constructed and ∼5700 genes (∼2200 on 4DS and ∼3500 on 4DL) predicted.The sequence and virtual order obtained here using the 454 platform were compared with the Illumina one used by the IWGSC, giving complementary information.

View Article: PubMed Central - PubMed

Affiliation: Estación Experimental Agropecuaria Marcos Juárez, Instituto Nacional de Tecnología Agropecuaria (INTA), Marcos Juárez, Córdoba, Argentina. Electronic address: helguera.marcelo@inta.gob.ar.

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Single end read length histograms for trimmed reads of T. aestivum chromosome 4D. (A) 4D short arm chromosome individual run profiles; (B) 4D short arm chromosome whole read data set; (C) 4D long arm chromosome individual run profiles; (D) 4D long arm chromosome whole read data set.
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fig0005: Single end read length histograms for trimmed reads of T. aestivum chromosome 4D. (A) 4D short arm chromosome individual run profiles; (B) 4D short arm chromosome whole read data set; (C) 4D long arm chromosome individual run profiles; (D) 4D long arm chromosome whole read data set.


New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing.

Helguera M, Rivarola M, Clavijo B, Martis MM, Vanzetti LS, González S, Garbus I, Leroy P, Šimková H, Valárik M, Caccamo M, Doležel J, Mayer KF, Feuillet C, Tranquilli G, Paniego N, Echenique V - Plant Sci. (2014)

Single end read length histograms for trimmed reads of T. aestivum chromosome 4D. (A) 4D short arm chromosome individual run profiles; (B) 4D short arm chromosome whole read data set; (C) 4D long arm chromosome individual run profiles; (D) 4D long arm chromosome whole read data set.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352925&req=5

fig0005: Single end read length histograms for trimmed reads of T. aestivum chromosome 4D. (A) 4D short arm chromosome individual run profiles; (B) 4D short arm chromosome whole read data set; (C) 4D long arm chromosome individual run profiles; (D) 4D long arm chromosome whole read data set.
Bottom Line: The virtual order showed a higher collinearity with homeologous 4B compared to 4A.Additionally, a virtual map was constructed and ∼5700 genes (∼2200 on 4DS and ∼3500 on 4DL) predicted.The sequence and virtual order obtained here using the 454 platform were compared with the Illumina one used by the IWGSC, giving complementary information.

View Article: PubMed Central - PubMed

Affiliation: Estación Experimental Agropecuaria Marcos Juárez, Instituto Nacional de Tecnología Agropecuaria (INTA), Marcos Juárez, Córdoba, Argentina. Electronic address: helguera.marcelo@inta.gob.ar.

Show MeSH