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New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing.

Helguera M, Rivarola M, Clavijo B, Martis MM, Vanzetti LS, González S, Garbus I, Leroy P, Šimková H, Valárik M, Caccamo M, Doležel J, Mayer KF, Feuillet C, Tranquilli G, Paniego N, Echenique V - Plant Sci. (2014)

Bottom Line: The virtual order showed a higher collinearity with homeologous 4B compared to 4A.Additionally, a virtual map was constructed and ∼5700 genes (∼2200 on 4DS and ∼3500 on 4DL) predicted.The sequence and virtual order obtained here using the 454 platform were compared with the Illumina one used by the IWGSC, giving complementary information.

View Article: PubMed Central - PubMed

Affiliation: Estación Experimental Agropecuaria Marcos Juárez, Instituto Nacional de Tecnología Agropecuaria (INTA), Marcos Juárez, Córdoba, Argentina. Electronic address: helguera.marcelo@inta.gob.ar.

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Venn diagram displaying the number of genes from T. aestivum 4D chromosome supported by other reference genomes. (A) 4DS genes supported by one (497 genes = 55.7%), two (174 genes = 19.5%) or three (221 genes = 24.8%) reference genomes. (B) 4DL genes supported by one (369 genes = 34.1%), two (250 genes = 23.2%) or three (462 genes = 42.7%) reference genomes. Bd: Brachypodium distachyon, Os: Oryza sativa (rice), Sb: Sorghum bicolor (sorghum).
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fig0040: Venn diagram displaying the number of genes from T. aestivum 4D chromosome supported by other reference genomes. (A) 4DS genes supported by one (497 genes = 55.7%), two (174 genes = 19.5%) or three (221 genes = 24.8%) reference genomes. (B) 4DL genes supported by one (369 genes = 34.1%), two (250 genes = 23.2%) or three (462 genes = 42.7%) reference genomes. Bd: Brachypodium distachyon, Os: Oryza sativa (rice), Sb: Sorghum bicolor (sorghum).


New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing.

Helguera M, Rivarola M, Clavijo B, Martis MM, Vanzetti LS, González S, Garbus I, Leroy P, Šimková H, Valárik M, Caccamo M, Doležel J, Mayer KF, Feuillet C, Tranquilli G, Paniego N, Echenique V - Plant Sci. (2014)

Venn diagram displaying the number of genes from T. aestivum 4D chromosome supported by other reference genomes. (A) 4DS genes supported by one (497 genes = 55.7%), two (174 genes = 19.5%) or three (221 genes = 24.8%) reference genomes. (B) 4DL genes supported by one (369 genes = 34.1%), two (250 genes = 23.2%) or three (462 genes = 42.7%) reference genomes. Bd: Brachypodium distachyon, Os: Oryza sativa (rice), Sb: Sorghum bicolor (sorghum).
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352925&req=5

fig0040: Venn diagram displaying the number of genes from T. aestivum 4D chromosome supported by other reference genomes. (A) 4DS genes supported by one (497 genes = 55.7%), two (174 genes = 19.5%) or three (221 genes = 24.8%) reference genomes. (B) 4DL genes supported by one (369 genes = 34.1%), two (250 genes = 23.2%) or three (462 genes = 42.7%) reference genomes. Bd: Brachypodium distachyon, Os: Oryza sativa (rice), Sb: Sorghum bicolor (sorghum).
Bottom Line: The virtual order showed a higher collinearity with homeologous 4B compared to 4A.Additionally, a virtual map was constructed and ∼5700 genes (∼2200 on 4DS and ∼3500 on 4DL) predicted.The sequence and virtual order obtained here using the 454 platform were compared with the Illumina one used by the IWGSC, giving complementary information.

View Article: PubMed Central - PubMed

Affiliation: Estación Experimental Agropecuaria Marcos Juárez, Instituto Nacional de Tecnología Agropecuaria (INTA), Marcos Juárez, Córdoba, Argentina. Electronic address: helguera.marcelo@inta.gob.ar.

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