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Influence of vitamin C supplementation on oxidative stress and neutrophil inflammatory response in acute and regular exercise.

Popovic LM, Mitic NR, Miric D, Bisevac B, Miric M, Popovic B - Oxid Med Cell Longev (2015)

Bottom Line: Our results showed increased postexercise Asc in serum independently of vitamin supplementation.They also showed that vitamin C can significantly decrease postexercise MDA level in both experimental groups.Increased postexercise MPO activity has been found in both groups and was not affected by vitamin C supplementation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathophysiology, Medical Faculty Pristina, 38220 Kosovska Mitrovica, Serbia.

ABSTRACT
Exercise induces a multitude of physiological and biochemical changes in blood affecting its redox status. Tissue damage resulting from exercise induces activation of inflammatory cells followed by the increased activity of myeloperoxidase (MPO) in circulation. Vitamin C readily scavenges free radicals and may thereby prevent oxidative damage of important biological macromolecules. The aim of this study was to examine the effect of vitamin C supplementation on oxidative stress and neutrophil inflammatory response induced by acute and regular exercise. Experiment was conducted on acute exercise group (performing Bruce Treadmill Protocol (BTP)) and regular training group. Markers of lipid peroxidation, malondialdehyde (MDA), MPO activity, and vitamin C status were estimated at rest and after BTP (acute exercise group) and before and after vitamin C supplementation in both groups. Our results showed increased postexercise Asc in serum independently of vitamin supplementation. They also showed that vitamin C can significantly decrease postexercise MDA level in both experimental groups. Increased postexercise MPO activity has been found in both groups and was not affected by vitamin C supplementation. We concluded that vitamin C supplementation can suppress lipid peroxidation process during exercise but cannot affect neutrophil inflammatory response in either exercise group.

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Myeloperoxidase activity in acute exercise group. Myeloperoxidase (MPO) activity was measured in serum before vitamin C supplementation in rest (A) and after BTP (A1), and after 14 days vitamin C supplementation in rest (B) and after BTP (B1).
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fig4: Myeloperoxidase activity in acute exercise group. Myeloperoxidase (MPO) activity was measured in serum before vitamin C supplementation in rest (A) and after BTP (A1), and after 14 days vitamin C supplementation in rest (B) and after BTP (B1).

Mentions: Results obtained in acute exercise group are shown in Figures 1–4. Changes of serum vitamin C in acute exercise group are presented in Figure 1. As can be seen, vitamin C basal levels increased from initial 55.40 μmol/L to 98.57 μmol/L after 14 days of supplementation, indicating that participants complied with given experimental protocol. We also observed that postexercise vitamin C increased independently of supplementation (55.40 versus 67.37 μmol/L before and 98.57 versus 114.97 μmol/L after supplementation). Both changes were statistically significant (P < 0.001).


Influence of vitamin C supplementation on oxidative stress and neutrophil inflammatory response in acute and regular exercise.

Popovic LM, Mitic NR, Miric D, Bisevac B, Miric M, Popovic B - Oxid Med Cell Longev (2015)

Myeloperoxidase activity in acute exercise group. Myeloperoxidase (MPO) activity was measured in serum before vitamin C supplementation in rest (A) and after BTP (A1), and after 14 days vitamin C supplementation in rest (B) and after BTP (B1).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352897&req=5

fig4: Myeloperoxidase activity in acute exercise group. Myeloperoxidase (MPO) activity was measured in serum before vitamin C supplementation in rest (A) and after BTP (A1), and after 14 days vitamin C supplementation in rest (B) and after BTP (B1).
Mentions: Results obtained in acute exercise group are shown in Figures 1–4. Changes of serum vitamin C in acute exercise group are presented in Figure 1. As can be seen, vitamin C basal levels increased from initial 55.40 μmol/L to 98.57 μmol/L after 14 days of supplementation, indicating that participants complied with given experimental protocol. We also observed that postexercise vitamin C increased independently of supplementation (55.40 versus 67.37 μmol/L before and 98.57 versus 114.97 μmol/L after supplementation). Both changes were statistically significant (P < 0.001).

Bottom Line: Our results showed increased postexercise Asc in serum independently of vitamin supplementation.They also showed that vitamin C can significantly decrease postexercise MDA level in both experimental groups.Increased postexercise MPO activity has been found in both groups and was not affected by vitamin C supplementation.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathophysiology, Medical Faculty Pristina, 38220 Kosovska Mitrovica, Serbia.

ABSTRACT
Exercise induces a multitude of physiological and biochemical changes in blood affecting its redox status. Tissue damage resulting from exercise induces activation of inflammatory cells followed by the increased activity of myeloperoxidase (MPO) in circulation. Vitamin C readily scavenges free radicals and may thereby prevent oxidative damage of important biological macromolecules. The aim of this study was to examine the effect of vitamin C supplementation on oxidative stress and neutrophil inflammatory response induced by acute and regular exercise. Experiment was conducted on acute exercise group (performing Bruce Treadmill Protocol (BTP)) and regular training group. Markers of lipid peroxidation, malondialdehyde (MDA), MPO activity, and vitamin C status were estimated at rest and after BTP (acute exercise group) and before and after vitamin C supplementation in both groups. Our results showed increased postexercise Asc in serum independently of vitamin supplementation. They also showed that vitamin C can significantly decrease postexercise MDA level in both experimental groups. Increased postexercise MPO activity has been found in both groups and was not affected by vitamin C supplementation. We concluded that vitamin C supplementation can suppress lipid peroxidation process during exercise but cannot affect neutrophil inflammatory response in either exercise group.

Show MeSH
Related in: MedlinePlus