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Jarid2 Methylation via the PRC2 Complex Regulates H3K27me3 Deposition during Cell Differentiation.

Sai S, Justin N, Teissandier A, Ancelin K, Portoso M, Caron M, Michaud A, Lombard B, da Rocha ST, Offer J, Loew D, Servant N, Wassef M, Burlina F, Gamblin SJ, Heard E, Margueron R - Mol. Cell (2015)

Bottom Line: This modification is recognized by the Eed core component of PRC2 and triggers an allosteric activation of PRC2's enzymatic activity.We show that Jarid2 methylation is important to promote PRC2 activity at a locus devoid of H3K27me3 and for the correct deposition of this mark during cell differentiation.Our results uncover a regulation loop where Jarid2 methylation fine-tunes PRC2 activity depending on the chromatin context.

View Article: PubMed Central - PubMed

Affiliation: Institut Curie, 26 Rue d'Ulm, 75005 Paris, France; INSERM U934, 26 Rue d'Ulm, 75005 Paris, France; CNRS UMR3215, 26 Rue d'Ulm, 75005 Paris, France.

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Jarid2 and Its Methylated Form Colocalize Almost Perfectly at Chromatin(A) Cellular fractionation of E14 ESC. Fractions are indicated on top and specific antibodies on the right.(B) Venn diagram showing the overlap (yellow) between peaks enriched for Jarid2 total and Jarid2-K116me2 or peaks specific for Jarid2 (red) or its methylated form (green) in E14 ESC.(C) Jarid2, Jarid2-K116me2, and H3K27me3 enrichments relative to TSS for common peaks.(D) Left: normalized read counts for Jarid2 versus Jarid2-K116me2 ChIP-seq at common target peaks. R2 = correlation coefficient. Right: zoom-in of the blue square area of left panel showing additionally read counts Jarid2 specific (red) and Jarid2-K116me2 specific (green). See also Figure S3.
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fig3: Jarid2 and Its Methylated Form Colocalize Almost Perfectly at Chromatin(A) Cellular fractionation of E14 ESC. Fractions are indicated on top and specific antibodies on the right.(B) Venn diagram showing the overlap (yellow) between peaks enriched for Jarid2 total and Jarid2-K116me2 or peaks specific for Jarid2 (red) or its methylated form (green) in E14 ESC.(C) Jarid2, Jarid2-K116me2, and H3K27me3 enrichments relative to TSS for common peaks.(D) Left: normalized read counts for Jarid2 versus Jarid2-K116me2 ChIP-seq at common target peaks. R2 = correlation coefficient. Right: zoom-in of the blue square area of left panel showing additionally read counts Jarid2 specific (red) and Jarid2-K116me2 specific (green). See also Figure S3.

Mentions: The IF experiments prompted us to study in more details the distribution of Jarid2-K116me2 at chromatin. We first analyzed the subcellular distribution of Jarid2-K116me2 by performing cell fractionation experiments followed by western blots. Both total Jarid2 and Jarid2-K116me2 are preferentially enriched in the insoluble chromatin fraction, although they are also detected in the other nuclear fractions (Figure 3A).


Jarid2 Methylation via the PRC2 Complex Regulates H3K27me3 Deposition during Cell Differentiation.

Sai S, Justin N, Teissandier A, Ancelin K, Portoso M, Caron M, Michaud A, Lombard B, da Rocha ST, Offer J, Loew D, Servant N, Wassef M, Burlina F, Gamblin SJ, Heard E, Margueron R - Mol. Cell (2015)

Jarid2 and Its Methylated Form Colocalize Almost Perfectly at Chromatin(A) Cellular fractionation of E14 ESC. Fractions are indicated on top and specific antibodies on the right.(B) Venn diagram showing the overlap (yellow) between peaks enriched for Jarid2 total and Jarid2-K116me2 or peaks specific for Jarid2 (red) or its methylated form (green) in E14 ESC.(C) Jarid2, Jarid2-K116me2, and H3K27me3 enrichments relative to TSS for common peaks.(D) Left: normalized read counts for Jarid2 versus Jarid2-K116me2 ChIP-seq at common target peaks. R2 = correlation coefficient. Right: zoom-in of the blue square area of left panel showing additionally read counts Jarid2 specific (red) and Jarid2-K116me2 specific (green). See also Figure S3.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4352895&req=5

fig3: Jarid2 and Its Methylated Form Colocalize Almost Perfectly at Chromatin(A) Cellular fractionation of E14 ESC. Fractions are indicated on top and specific antibodies on the right.(B) Venn diagram showing the overlap (yellow) between peaks enriched for Jarid2 total and Jarid2-K116me2 or peaks specific for Jarid2 (red) or its methylated form (green) in E14 ESC.(C) Jarid2, Jarid2-K116me2, and H3K27me3 enrichments relative to TSS for common peaks.(D) Left: normalized read counts for Jarid2 versus Jarid2-K116me2 ChIP-seq at common target peaks. R2 = correlation coefficient. Right: zoom-in of the blue square area of left panel showing additionally read counts Jarid2 specific (red) and Jarid2-K116me2 specific (green). See also Figure S3.
Mentions: The IF experiments prompted us to study in more details the distribution of Jarid2-K116me2 at chromatin. We first analyzed the subcellular distribution of Jarid2-K116me2 by performing cell fractionation experiments followed by western blots. Both total Jarid2 and Jarid2-K116me2 are preferentially enriched in the insoluble chromatin fraction, although they are also detected in the other nuclear fractions (Figure 3A).

Bottom Line: This modification is recognized by the Eed core component of PRC2 and triggers an allosteric activation of PRC2's enzymatic activity.We show that Jarid2 methylation is important to promote PRC2 activity at a locus devoid of H3K27me3 and for the correct deposition of this mark during cell differentiation.Our results uncover a regulation loop where Jarid2 methylation fine-tunes PRC2 activity depending on the chromatin context.

View Article: PubMed Central - PubMed

Affiliation: Institut Curie, 26 Rue d'Ulm, 75005 Paris, France; INSERM U934, 26 Rue d'Ulm, 75005 Paris, France; CNRS UMR3215, 26 Rue d'Ulm, 75005 Paris, France.

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Related in: MedlinePlus