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Specific microRNAs regulate heat stress responses in Caenorhabditis elegans.

Nehammer C, Podolska A, Mackowiak SD, Kagias K, Pocock R - Sci Rep (2015)

Bottom Line: The ability of animals to sense and respond to elevated temperature is essential for survival.Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress.These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

View Article: PubMed Central - PubMed

Affiliation: Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen, Denmark.

ABSTRACT
The ability of animals to sense and respond to elevated temperature is essential for survival. Transcriptional control of the heat stress response has been much studied, whereas its posttranscriptional regulation by microRNAs (miRNAs) is not well understood. Here we analyzed the miRNA response to heat stress in Caenorhabditis elegans and show that a discrete subset of miRNAs is thermoregulated. Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress. We have identified additional functions for already known players (mir-71 and mir-239) as well as identifying mir-80 and the mir-229 mir-64-66 cluster as important regulators of the heat stress response in C. elegans. These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

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C. elegans Reduces Motility in Elevated Temperature.(a–c) Synchronized L4 animals were exposed to 20°C or 30°C for 6 hrs, followed by 16 hrs on plates fully covered with bacteria to assay motility. Data is presented as number of squares the worm crossed within 16 hrs out of a total of 240 squares. Data are presented as means ± SEM and statistical significance was assessed by ANOVA followed by Dunnett's multiple comparison test n > 13, *p < 0.05. The wild-type worms presented in (a) are the same as for (b) for illustration.
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f3: C. elegans Reduces Motility in Elevated Temperature.(a–c) Synchronized L4 animals were exposed to 20°C or 30°C for 6 hrs, followed by 16 hrs on plates fully covered with bacteria to assay motility. Data is presented as number of squares the worm crossed within 16 hrs out of a total of 240 squares. Data are presented as means ± SEM and statistical significance was assessed by ANOVA followed by Dunnett's multiple comparison test n > 13, *p < 0.05. The wild-type worms presented in (a) are the same as for (b) for illustration.

Mentions: It has been shown that reduction of energy metabolism extends lifespan and stress resistance in C. elegans3334. One way of reducing energy demand would be to reduce motility. We tested this hypothesis by placing single worms on fully coated bacterial plates at either 20°C or 30°C for 16 hrs and measured their motility (Figure 3). We found that wild type animals reduce their motility by almost 10-fold at 30°C (Figure 3A). We next tested whether TRM mutant strains exhibit altered motility at either 20°C or 30°C when compared to wild type. We found that all strains behaved like wild type except for the mir-239 mutant, which showed and increase in motility compared to wild type at 30°C (Figure 3). The reason for this increase in motility in the mir-239 mutant is unclear and would require further study.


Specific microRNAs regulate heat stress responses in Caenorhabditis elegans.

Nehammer C, Podolska A, Mackowiak SD, Kagias K, Pocock R - Sci Rep (2015)

C. elegans Reduces Motility in Elevated Temperature.(a–c) Synchronized L4 animals were exposed to 20°C or 30°C for 6 hrs, followed by 16 hrs on plates fully covered with bacteria to assay motility. Data is presented as number of squares the worm crossed within 16 hrs out of a total of 240 squares. Data are presented as means ± SEM and statistical significance was assessed by ANOVA followed by Dunnett's multiple comparison test n > 13, *p < 0.05. The wild-type worms presented in (a) are the same as for (b) for illustration.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352874&req=5

f3: C. elegans Reduces Motility in Elevated Temperature.(a–c) Synchronized L4 animals were exposed to 20°C or 30°C for 6 hrs, followed by 16 hrs on plates fully covered with bacteria to assay motility. Data is presented as number of squares the worm crossed within 16 hrs out of a total of 240 squares. Data are presented as means ± SEM and statistical significance was assessed by ANOVA followed by Dunnett's multiple comparison test n > 13, *p < 0.05. The wild-type worms presented in (a) are the same as for (b) for illustration.
Mentions: It has been shown that reduction of energy metabolism extends lifespan and stress resistance in C. elegans3334. One way of reducing energy demand would be to reduce motility. We tested this hypothesis by placing single worms on fully coated bacterial plates at either 20°C or 30°C for 16 hrs and measured their motility (Figure 3). We found that wild type animals reduce their motility by almost 10-fold at 30°C (Figure 3A). We next tested whether TRM mutant strains exhibit altered motility at either 20°C or 30°C when compared to wild type. We found that all strains behaved like wild type except for the mir-239 mutant, which showed and increase in motility compared to wild type at 30°C (Figure 3). The reason for this increase in motility in the mir-239 mutant is unclear and would require further study.

Bottom Line: The ability of animals to sense and respond to elevated temperature is essential for survival.Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress.These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

View Article: PubMed Central - PubMed

Affiliation: Biotech Research and Innovation Centre, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen, Denmark.

ABSTRACT
The ability of animals to sense and respond to elevated temperature is essential for survival. Transcriptional control of the heat stress response has been much studied, whereas its posttranscriptional regulation by microRNAs (miRNAs) is not well understood. Here we analyzed the miRNA response to heat stress in Caenorhabditis elegans and show that a discrete subset of miRNAs is thermoregulated. Using in-depth phenotypic analyses of miRNA deletion mutant strains we reveal multiple developmental and post-developmental survival and behavioral functions for specific miRNAs during heat stress. We have identified additional functions for already known players (mir-71 and mir-239) as well as identifying mir-80 and the mir-229 mir-64-66 cluster as important regulators of the heat stress response in C. elegans. These findings uncover an additional layer of complexity to the regulation of stress signaling that enables animals to robustly respond to the changing environment.

Show MeSH
Related in: MedlinePlus