Error-free DNA damage tolerance and sister chromatid proximity during DNA replication rely on the Polα/Primase/Ctf4 Complex.
Bottom Line: We show that Saccharomyces cerevisiae Polα/Primase/Ctf4 mutants, proficient in bulk DNA replication, are defective in recombination-mediated damage-bypass by template switching (TS) and have reduced sister chromatid cohesion.The decrease in error-free DDT is accompanied by increased usage of mutagenic DDT, fork reversal, and higher rates of genome rearrangements mediated by faulty strand annealing.Defects in this event impact on replication fork architecture and sister chromatid proximity, and represent a frequent source of chromosome lesions upon replication dysfunctions.
Affiliation: IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy.Show MeSH
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Affiliation: IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, Italy.