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Effect of salinity on biomass yield and physiological and stem-root anatomical characteristics of purslane (Portulaca oleracea L.) accessions.

Alam MA, Juraimi AS, Rafii MY, Abdul Hamid A - Biomed Res Int (2015)

Bottom Line: Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes.Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels.Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.

ABSTRACT
13 selected purslane accessions were subjected to five salinity levels 0, 8, 16, 24, and 32 dS m(-1). Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes. Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels. The fresh and dry matter production was found to increase in Ac1, Ac9, and Ac13 from lower to higher salinity levels but others were badly affected. Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control. Anatomically, stem cortical tissues of Ac5, Ac9, and Ac12 were unaffected at control and 8 dS m(-1) salinity but root cortical tissues did not show any significant damage except a bit enlargement in Ac12 and Ac13. A dendrogram was constructed by UPGMA based on biomass yield and physiological traits where all 13 accessions were grouped into 5 clusters proving greater diversity among them. The 3-dimensional principal component analysis (PCA) has also confirmed the output of grouping from cluster analysis. Overall, salinity stressed among all 13 purslane accessions considering biomass production, physiological growth, and anatomical development Ac9 was the best salt-tolerant purslane accession and Ac13 was the most affected accession.

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Related in: MedlinePlus

Scanning electron micrographs showing salinity effects on purslane root histology of Ac4.
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fig9: Scanning electron micrographs showing salinity effects on purslane root histology of Ac4.

Mentions: Electron microscopy observation of the transverse section of stems induced by different salinity levels revealed significant changes in anatomical structures. The most affected parts were cortical tissues (C) compared to other sections. The cell damage may result from stem cortex cell collapse due to salt stress. Stem cortical cells of all the 13 purslane accessions were affected at moderate to the highest salinity stress (16 dS m−1, 24 dS m−1, and 32 dS m−1), whereas root cortical tissues were not affected by augmented salinity levels in case of all the accessions except Ac13. Meanwhile, stem cortical cells of Ac5, Ac9, and Ac12 did not show cell collapse with 0 and 8 dS m−1 salinity treatments but later with the increased salinity stress the cortical cell collapse was also increased and the severe cell collapse was observed at the highest salinity (32 dS m−1) stress (Figures 6, 7, and 8). More or less the similar results have also been observed in case of stem histology of all other purslane accessions (figure not shown). The root transverse section of Ac4, Ac5, Ac7, and Ac10 did not show any detrimental changes or cell collapse in the cortical tissues at all the salinity levels from 0 dS m−1 to 32 dS m−1 (Figures 9 and 10) but a slight enlargement of the cortical cells was observed in Ac12 at 32 dS m−1 salinity (Figure 11), whereas the same incident was found in Ac13 at the salinity treatments 16 dS m−1, 24 dS m−1, and 32 dS m−1 (Figure 12).


Effect of salinity on biomass yield and physiological and stem-root anatomical characteristics of purslane (Portulaca oleracea L.) accessions.

Alam MA, Juraimi AS, Rafii MY, Abdul Hamid A - Biomed Res Int (2015)

Scanning electron micrographs showing salinity effects on purslane root histology of Ac4.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352753&req=5

fig9: Scanning electron micrographs showing salinity effects on purslane root histology of Ac4.
Mentions: Electron microscopy observation of the transverse section of stems induced by different salinity levels revealed significant changes in anatomical structures. The most affected parts were cortical tissues (C) compared to other sections. The cell damage may result from stem cortex cell collapse due to salt stress. Stem cortical cells of all the 13 purslane accessions were affected at moderate to the highest salinity stress (16 dS m−1, 24 dS m−1, and 32 dS m−1), whereas root cortical tissues were not affected by augmented salinity levels in case of all the accessions except Ac13. Meanwhile, stem cortical cells of Ac5, Ac9, and Ac12 did not show cell collapse with 0 and 8 dS m−1 salinity treatments but later with the increased salinity stress the cortical cell collapse was also increased and the severe cell collapse was observed at the highest salinity (32 dS m−1) stress (Figures 6, 7, and 8). More or less the similar results have also been observed in case of stem histology of all other purslane accessions (figure not shown). The root transverse section of Ac4, Ac5, Ac7, and Ac10 did not show any detrimental changes or cell collapse in the cortical tissues at all the salinity levels from 0 dS m−1 to 32 dS m−1 (Figures 9 and 10) but a slight enlargement of the cortical cells was observed in Ac12 at 32 dS m−1 salinity (Figure 11), whereas the same incident was found in Ac13 at the salinity treatments 16 dS m−1, 24 dS m−1, and 32 dS m−1 (Figure 12).

Bottom Line: Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes.Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels.Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.

ABSTRACT
13 selected purslane accessions were subjected to five salinity levels 0, 8, 16, 24, and 32 dS m(-1). Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes. Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels. The fresh and dry matter production was found to increase in Ac1, Ac9, and Ac13 from lower to higher salinity levels but others were badly affected. Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control. Anatomically, stem cortical tissues of Ac5, Ac9, and Ac12 were unaffected at control and 8 dS m(-1) salinity but root cortical tissues did not show any significant damage except a bit enlargement in Ac12 and Ac13. A dendrogram was constructed by UPGMA based on biomass yield and physiological traits where all 13 accessions were grouped into 5 clusters proving greater diversity among them. The 3-dimensional principal component analysis (PCA) has also confirmed the output of grouping from cluster analysis. Overall, salinity stressed among all 13 purslane accessions considering biomass production, physiological growth, and anatomical development Ac9 was the best salt-tolerant purslane accession and Ac13 was the most affected accession.

Show MeSH
Related in: MedlinePlus