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Effect of salinity on biomass yield and physiological and stem-root anatomical characteristics of purslane (Portulaca oleracea L.) accessions.

Alam MA, Juraimi AS, Rafii MY, Abdul Hamid A - Biomed Res Int (2015)

Bottom Line: Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes.Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels.Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.

ABSTRACT
13 selected purslane accessions were subjected to five salinity levels 0, 8, 16, 24, and 32 dS m(-1). Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes. Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels. The fresh and dry matter production was found to increase in Ac1, Ac9, and Ac13 from lower to higher salinity levels but others were badly affected. Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control. Anatomically, stem cortical tissues of Ac5, Ac9, and Ac12 were unaffected at control and 8 dS m(-1) salinity but root cortical tissues did not show any significant damage except a bit enlargement in Ac12 and Ac13. A dendrogram was constructed by UPGMA based on biomass yield and physiological traits where all 13 accessions were grouped into 5 clusters proving greater diversity among them. The 3-dimensional principal component analysis (PCA) has also confirmed the output of grouping from cluster analysis. Overall, salinity stressed among all 13 purslane accessions considering biomass production, physiological growth, and anatomical development Ac9 was the best salt-tolerant purslane accession and Ac13 was the most affected accession.

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Related in: MedlinePlus

Effect of salinity on total chlorophyll content of 13 purslane accessions. Each bar represents mean values (±SE) of three replicates. Mean values with different lower case letters within a group (i.e., accessions) are significantly different at P < 0.05.
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fig1: Effect of salinity on total chlorophyll content of 13 purslane accessions. Each bar represents mean values (±SE) of three replicates. Mean values with different lower case letters within a group (i.e., accessions) are significantly different at P < 0.05.

Mentions: Among all 13 purslane accessions total chlorophyll contents in untreated control plants were greatly significant (P < 0.0001) and ranged from 25.2 to 38.74 SPAD value; the highest chlorophyll content was recorded in Ac9 and the lowest was recorded both in Ac1 and in Ac5 (Figure 1). Chlorophyll contents in Ac3, Ac4, and Ac8 were found at 33.3, 33.4, and 33.0 SPAD value, respectively, which was not statistically significant. Chlorophyll contents in salt treated 13 purslane accessions were also reduced consequently with the increasing of salinity levels. In contrast, a bit increase in chlorophyll contents was also noted in Ac2 at 8 and 16 dS m−1, in Ac4 and Ac6 at 16 dS m−1, and in Ac8 at 8 and 16 dS m−1 salinity stress (Figure 1). The overall highest reduction (39.44%) in chlorophyll contents was observed in Ac7 at the highest levels 32 dS m−1 salinity with the lowest reduction (0.32%) in Ac11 at 8 dS m−1 salinity, while the highest increase (3.64%) in chlorophyll contents was found in Ac8 at 8 dS m−1 salinity with the lowest increase (1.82%) in the same accessions at 16 dS m−1 salinity levels (Table 3). On average over all accessions, 2.59% reduction, 1.17% increase, 11.21% reduction, and 18.79% reduction in total chlorophyll contents were recorded correspondingly at 8, 16, 24, and 32 dS m−1 salinity, which were statistically significant (P < 0.05, Table 3).


Effect of salinity on biomass yield and physiological and stem-root anatomical characteristics of purslane (Portulaca oleracea L.) accessions.

Alam MA, Juraimi AS, Rafii MY, Abdul Hamid A - Biomed Res Int (2015)

Effect of salinity on total chlorophyll content of 13 purslane accessions. Each bar represents mean values (±SE) of three replicates. Mean values with different lower case letters within a group (i.e., accessions) are significantly different at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352753&req=5

fig1: Effect of salinity on total chlorophyll content of 13 purslane accessions. Each bar represents mean values (±SE) of three replicates. Mean values with different lower case letters within a group (i.e., accessions) are significantly different at P < 0.05.
Mentions: Among all 13 purslane accessions total chlorophyll contents in untreated control plants were greatly significant (P < 0.0001) and ranged from 25.2 to 38.74 SPAD value; the highest chlorophyll content was recorded in Ac9 and the lowest was recorded both in Ac1 and in Ac5 (Figure 1). Chlorophyll contents in Ac3, Ac4, and Ac8 were found at 33.3, 33.4, and 33.0 SPAD value, respectively, which was not statistically significant. Chlorophyll contents in salt treated 13 purslane accessions were also reduced consequently with the increasing of salinity levels. In contrast, a bit increase in chlorophyll contents was also noted in Ac2 at 8 and 16 dS m−1, in Ac4 and Ac6 at 16 dS m−1, and in Ac8 at 8 and 16 dS m−1 salinity stress (Figure 1). The overall highest reduction (39.44%) in chlorophyll contents was observed in Ac7 at the highest levels 32 dS m−1 salinity with the lowest reduction (0.32%) in Ac11 at 8 dS m−1 salinity, while the highest increase (3.64%) in chlorophyll contents was found in Ac8 at 8 dS m−1 salinity with the lowest increase (1.82%) in the same accessions at 16 dS m−1 salinity levels (Table 3). On average over all accessions, 2.59% reduction, 1.17% increase, 11.21% reduction, and 18.79% reduction in total chlorophyll contents were recorded correspondingly at 8, 16, 24, and 32 dS m−1 salinity, which were statistically significant (P < 0.05, Table 3).

Bottom Line: Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes.Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels.Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control.

View Article: PubMed Central - PubMed

Affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.

ABSTRACT
13 selected purslane accessions were subjected to five salinity levels 0, 8, 16, 24, and 32 dS m(-1). Salinity effect was evaluated on the basis of biomass yield reduction, physiological attributes, and stem-root anatomical changes. Aggravated salinity stress caused significant (P < 0.05) reduction in all measured parameters and the highest salinity showed more detrimental effect compared to control as well as lower salinity levels. The fresh and dry matter production was found to increase in Ac1, Ac9, and Ac13 from lower to higher salinity levels but others were badly affected. Considering salinity effect on purslane physiology, increase in chlorophyll content was seen in Ac2, Ac4, Ac6, and Ac8 at 16 dS m(-1) salinity, whereas Ac4, Ac9, and Ac12 showed increased photosynthesis at the same salinity levels compared to control. Anatomically, stem cortical tissues of Ac5, Ac9, and Ac12 were unaffected at control and 8 dS m(-1) salinity but root cortical tissues did not show any significant damage except a bit enlargement in Ac12 and Ac13. A dendrogram was constructed by UPGMA based on biomass yield and physiological traits where all 13 accessions were grouped into 5 clusters proving greater diversity among them. The 3-dimensional principal component analysis (PCA) has also confirmed the output of grouping from cluster analysis. Overall, salinity stressed among all 13 purslane accessions considering biomass production, physiological growth, and anatomical development Ac9 was the best salt-tolerant purslane accession and Ac13 was the most affected accession.

Show MeSH
Related in: MedlinePlus