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Ethnic-specific genetic association of variants in the corticotropin-releasing hormone receptor 1 gene with nicotine dependence.

Tang X, Zhan S, Yang L, Cui W, Ma JZ, Payne TJ, Li MD - Biomed Res Int (2015)

Bottom Line: Association analysis of the five SNPs revealed a significant association of rs171440 with SQ in the AA sample and with SQ and FTND in the pooled AA and EA samples.However, none of these associations remained significant after correction for multiple testing.Together, our results provide suggestive evidence for the involvement of CRHR1 in ND, which warrants further investigation using larger independent samples.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou 310009, China.

ABSTRACT
Twin and family studies indicate that smoking addiction is highly influenced by genetic factors. Variants in the corticotropin-releasing hormone receptor 1 (CRHR1) gene have been associated with alcoholism and depression. In this study, we tested five single nucleotide polymorphisms (SNPs) in CRHR1 for their association with ND, which was assessed by smoking quantity (SQ), the Heaviness of Smoking Index (HSI), and the Fagerström test for ND (FTND) in 2,037 subjects from 602 families of either European American (EA) or African American (AA) ancestry. Association analysis of the five SNPs revealed a significant association of rs171440 with SQ in the AA sample and with SQ and FTND in the pooled AA and EA samples. Haplotype-based association analysis indicated significant association of haplotypes C-C (56.9%) and T-C (38.9%), formed by SNPs rs171440 and rs1396862, with SQ in the AA sample, C-C-G (47.6%) with SQ, and T-C-G (42.3%), formed by SNPs rs171440, rs1396862, and rs878886, with SQ and FTND in the pooled AA and EA samples. However, none of these associations remained significant after correction for multiple testing. Together, our results provide suggestive evidence for the involvement of CRHR1 in ND, which warrants further investigation using larger independent samples.

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Related in: MedlinePlus

LD structure of five SNPs in CRHR1 in the pooled, AA, and EA samples. Haplotype block in each sample was defined according to Gabriel et al. [11].
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Related In: Results  -  Collection


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fig1: LD structure of five SNPs in CRHR1 in the pooled, AA, and EA samples. Haplotype block in each sample was defined according to Gabriel et al. [11].

Mentions: We used the Haploview program to calculate the pair-wise D′ values for the five SNPs in CRHR1 for all the three samples. According to the criteria of Gabriel et al. [11], all five SNPs in CRHR1 were assigned to an LD block in the EA sample, SNPs rs171440 and rs1396862 to an LD block for the AA sample, and SNPs rs171440, rs1396862, and rs878886 to an LD block for the pooled AA and EA samples (Figure 1). Such LD results indicated a genetic architecture difference in CRHR1 in the AA and EA samples.


Ethnic-specific genetic association of variants in the corticotropin-releasing hormone receptor 1 gene with nicotine dependence.

Tang X, Zhan S, Yang L, Cui W, Ma JZ, Payne TJ, Li MD - Biomed Res Int (2015)

LD structure of five SNPs in CRHR1 in the pooled, AA, and EA samples. Haplotype block in each sample was defined according to Gabriel et al. [11].
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352749&req=5

fig1: LD structure of five SNPs in CRHR1 in the pooled, AA, and EA samples. Haplotype block in each sample was defined according to Gabriel et al. [11].
Mentions: We used the Haploview program to calculate the pair-wise D′ values for the five SNPs in CRHR1 for all the three samples. According to the criteria of Gabriel et al. [11], all five SNPs in CRHR1 were assigned to an LD block in the EA sample, SNPs rs171440 and rs1396862 to an LD block for the AA sample, and SNPs rs171440, rs1396862, and rs878886 to an LD block for the pooled AA and EA samples (Figure 1). Such LD results indicated a genetic architecture difference in CRHR1 in the AA and EA samples.

Bottom Line: Association analysis of the five SNPs revealed a significant association of rs171440 with SQ in the AA sample and with SQ and FTND in the pooled AA and EA samples.However, none of these associations remained significant after correction for multiple testing.Together, our results provide suggestive evidence for the involvement of CRHR1 in ND, which warrants further investigation using larger independent samples.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou 310009, China.

ABSTRACT
Twin and family studies indicate that smoking addiction is highly influenced by genetic factors. Variants in the corticotropin-releasing hormone receptor 1 (CRHR1) gene have been associated with alcoholism and depression. In this study, we tested five single nucleotide polymorphisms (SNPs) in CRHR1 for their association with ND, which was assessed by smoking quantity (SQ), the Heaviness of Smoking Index (HSI), and the Fagerström test for ND (FTND) in 2,037 subjects from 602 families of either European American (EA) or African American (AA) ancestry. Association analysis of the five SNPs revealed a significant association of rs171440 with SQ in the AA sample and with SQ and FTND in the pooled AA and EA samples. Haplotype-based association analysis indicated significant association of haplotypes C-C (56.9%) and T-C (38.9%), formed by SNPs rs171440 and rs1396862, with SQ in the AA sample, C-C-G (47.6%) with SQ, and T-C-G (42.3%), formed by SNPs rs171440, rs1396862, and rs878886, with SQ and FTND in the pooled AA and EA samples. However, none of these associations remained significant after correction for multiple testing. Together, our results provide suggestive evidence for the involvement of CRHR1 in ND, which warrants further investigation using larger independent samples.

Show MeSH
Related in: MedlinePlus