Limits...
Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhea.

Ling Z, Liu X, Cheng Y, Luo Y, Yuan L, Li L, Xiang C - Biomed Res Int (2015)

Bottom Line: Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation.Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD.This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.

ABSTRACT
Antibiotic-associated diarrhea (AAD) is one of the most common complications of most types of antibiotics. Our aim was to determine the efficacy of Clostridium butyricum, Bifidobacterium infantis, and their mixture for AAD treatment in mice. AAD models were administered with single probiotic strain and probiotic mixture for short term and long term to evaluate the changes of the composition and diversity of intestinal microbiota, histopathology of the colon, and the systemic inflammation. Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation. All predominant fecal bacteria reached normal level after the long-term probiotic mixture treatment, while IL-10, IFN-γ, and TNF-α also returned to normal level. However, the efficacy for AAD was time dependent and probiotic strain specific. Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD. In addition, the beneficial effects of C. butyricum combined with B. infantis probiotic mixture were superior to their single strain. This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

No MeSH data available.


Related in: MedlinePlus

Comparison of cytokines among the six groups. P < 0.05 was labeled. ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352745&req=5

fig4: Comparison of cytokines among the six groups. P < 0.05 was labeled. ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.

Mentions: In the AAD mice model, the TNF-α level increased and the IL-10 and IFN-γ levels decreased significantly, which might be involved in the systemic inflammation of the mice (Figure 4). However, IL-1β was not significantly altered in the AAD mice. The serum levels of IL-10 and IFN-γ increased significantly after long-term C. butyricum administration, while the TNF-α decreased obviously, which were also observed in the B. infantis group (P < 0.05). However, those altered serum IL-10, IFN-γ, and TNF-α were still not reaching healthy levels in the B. infantis group, whereas only IL-10 was still lower in the C. butyricum group than that in healthy mice. It was unexpected that the concentration of IL-10, IFN-γ, and TNF-α returned to normal levels in the probiotic mixture group (P > 0.05). Our results indicated that the C. butyricum combined with B. infantis could attenuate systemic inflammation in the AAD mice.


Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhea.

Ling Z, Liu X, Cheng Y, Luo Y, Yuan L, Li L, Xiang C - Biomed Res Int (2015)

Comparison of cytokines among the six groups. P < 0.05 was labeled. ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352745&req=5

fig4: Comparison of cytokines among the six groups. P < 0.05 was labeled. ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.
Mentions: In the AAD mice model, the TNF-α level increased and the IL-10 and IFN-γ levels decreased significantly, which might be involved in the systemic inflammation of the mice (Figure 4). However, IL-1β was not significantly altered in the AAD mice. The serum levels of IL-10 and IFN-γ increased significantly after long-term C. butyricum administration, while the TNF-α decreased obviously, which were also observed in the B. infantis group (P < 0.05). However, those altered serum IL-10, IFN-γ, and TNF-α were still not reaching healthy levels in the B. infantis group, whereas only IL-10 was still lower in the C. butyricum group than that in healthy mice. It was unexpected that the concentration of IL-10, IFN-γ, and TNF-α returned to normal levels in the probiotic mixture group (P > 0.05). Our results indicated that the C. butyricum combined with B. infantis could attenuate systemic inflammation in the AAD mice.

Bottom Line: Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation.Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD.This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.

ABSTRACT
Antibiotic-associated diarrhea (AAD) is one of the most common complications of most types of antibiotics. Our aim was to determine the efficacy of Clostridium butyricum, Bifidobacterium infantis, and their mixture for AAD treatment in mice. AAD models were administered with single probiotic strain and probiotic mixture for short term and long term to evaluate the changes of the composition and diversity of intestinal microbiota, histopathology of the colon, and the systemic inflammation. Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation. All predominant fecal bacteria reached normal level after the long-term probiotic mixture treatment, while IL-10, IFN-γ, and TNF-α also returned to normal level. However, the efficacy for AAD was time dependent and probiotic strain specific. Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD. In addition, the beneficial effects of C. butyricum combined with B. infantis probiotic mixture were superior to their single strain. This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

No MeSH data available.


Related in: MedlinePlus