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Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhea.

Ling Z, Liu X, Cheng Y, Luo Y, Yuan L, Li L, Xiang C - Biomed Res Int (2015)

Bottom Line: Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation.Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD.This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.

ABSTRACT
Antibiotic-associated diarrhea (AAD) is one of the most common complications of most types of antibiotics. Our aim was to determine the efficacy of Clostridium butyricum, Bifidobacterium infantis, and their mixture for AAD treatment in mice. AAD models were administered with single probiotic strain and probiotic mixture for short term and long term to evaluate the changes of the composition and diversity of intestinal microbiota, histopathology of the colon, and the systemic inflammation. Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation. All predominant fecal bacteria reached normal level after the long-term probiotic mixture treatment, while IL-10, IFN-γ, and TNF-α also returned to normal level. However, the efficacy for AAD was time dependent and probiotic strain specific. Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD. In addition, the beneficial effects of C. butyricum combined with B. infantis probiotic mixture were superior to their single strain. This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

No MeSH data available.


Related in: MedlinePlus

Histopathology of the colon after treatment. Images represent sections of the distal colon (magnification, ×40). (a) Healthy control; (b) AAD mice model; (c) saline control; (d) C. butyricum treated group; (e) B. infantis treated group; (f) probiotic mixture treated group.
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fig3: Histopathology of the colon after treatment. Images represent sections of the distal colon (magnification, ×40). (a) Healthy control; (b) AAD mice model; (c) saline control; (d) C. butyricum treated group; (e) B. infantis treated group; (f) probiotic mixture treated group.

Mentions: The evident damage of colon architecture after antibiotic treatment was loss of homogenously distributed and integrated villi (Figure 3). Most of the colon epithelial cells showed severe swelling and partially rupturing. After 15-day actively treatment, the tissue architecture of the colon was restored significantly in the C. butyricum combined with B. infantis group, with the villi homogenously distributing as the healthy control. However, the severe villous swelling and extending could still not return to normal level in the C. butyricum group and the B. infantis group. Our data suggested that the C. butyricum combined with B. infantis could help to restore the colon mucosa successfully in a relative long-term course.


Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhea.

Ling Z, Liu X, Cheng Y, Luo Y, Yuan L, Li L, Xiang C - Biomed Res Int (2015)

Histopathology of the colon after treatment. Images represent sections of the distal colon (magnification, ×40). (a) Healthy control; (b) AAD mice model; (c) saline control; (d) C. butyricum treated group; (e) B. infantis treated group; (f) probiotic mixture treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352745&req=5

fig3: Histopathology of the colon after treatment. Images represent sections of the distal colon (magnification, ×40). (a) Healthy control; (b) AAD mice model; (c) saline control; (d) C. butyricum treated group; (e) B. infantis treated group; (f) probiotic mixture treated group.
Mentions: The evident damage of colon architecture after antibiotic treatment was loss of homogenously distributed and integrated villi (Figure 3). Most of the colon epithelial cells showed severe swelling and partially rupturing. After 15-day actively treatment, the tissue architecture of the colon was restored significantly in the C. butyricum combined with B. infantis group, with the villi homogenously distributing as the healthy control. However, the severe villous swelling and extending could still not return to normal level in the C. butyricum group and the B. infantis group. Our data suggested that the C. butyricum combined with B. infantis could help to restore the colon mucosa successfully in a relative long-term course.

Bottom Line: Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation.Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD.This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.

ABSTRACT
Antibiotic-associated diarrhea (AAD) is one of the most common complications of most types of antibiotics. Our aim was to determine the efficacy of Clostridium butyricum, Bifidobacterium infantis, and their mixture for AAD treatment in mice. AAD models were administered with single probiotic strain and probiotic mixture for short term and long term to evaluate the changes of the composition and diversity of intestinal microbiota, histopathology of the colon, and the systemic inflammation. Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation. All predominant fecal bacteria reached normal level after the long-term probiotic mixture treatment, while IL-10, IFN-γ, and TNF-α also returned to normal level. However, the efficacy for AAD was time dependent and probiotic strain specific. Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD. In addition, the beneficial effects of C. butyricum combined with B. infantis probiotic mixture were superior to their single strain. This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

No MeSH data available.


Related in: MedlinePlus