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Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhea.

Ling Z, Liu X, Cheng Y, Luo Y, Yuan L, Li L, Xiang C - Biomed Res Int (2015)

Bottom Line: Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation.Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD.This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.

ABSTRACT
Antibiotic-associated diarrhea (AAD) is one of the most common complications of most types of antibiotics. Our aim was to determine the efficacy of Clostridium butyricum, Bifidobacterium infantis, and their mixture for AAD treatment in mice. AAD models were administered with single probiotic strain and probiotic mixture for short term and long term to evaluate the changes of the composition and diversity of intestinal microbiota, histopathology of the colon, and the systemic inflammation. Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation. All predominant fecal bacteria reached normal level after the long-term probiotic mixture treatment, while IL-10, IFN-γ, and TNF-α also returned to normal level. However, the efficacy for AAD was time dependent and probiotic strain specific. Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD. In addition, the beneficial effects of C. butyricum combined with B. infantis probiotic mixture were superior to their single strain. This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

No MeSH data available.


Related in: MedlinePlus

Bacterial loads in fecal microbiota as measured by qPCR (log10 copies/g fresh feces). The total bacteria were detected in the short-term and long-term treatment, while other predominant fecal microbiota were only detected in long-term treatment. Graph values are reported as the mean and standard deviation of the mean. Comparisons among the groups were calculated with Student's t-tests. P < 0.05 was labeled; ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.
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fig2: Bacterial loads in fecal microbiota as measured by qPCR (log10 copies/g fresh feces). The total bacteria were detected in the short-term and long-term treatment, while other predominant fecal microbiota were only detected in long-term treatment. Graph values are reported as the mean and standard deviation of the mean. Comparisons among the groups were calculated with Student's t-tests. P < 0.05 was labeled; ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.

Mentions: The differences in predominant fecal bacteria after treatment with different probiotic strains and different terms were detected by qPCR (Figure 2). After 5-day treatment, the total bacteria in these treated groups could not reach healthy levels, with approximately one order of magnitude decrease when compared with the healthy mice. However, the total bacteria returned to normal level after 15-day treatment except the saline control group, which indicated that long-term administration of probiotic strains or probiotic mixture demonstrated a positive effect on modulating the intestinal microbiota in mice. In order to explore the specific modulation of the fecal microbiota, other nine predominant bacteria were detected after 15-day treatment. Our data demonstrated that Bacteroides-Prevotella group, Clostridium cluster XI, Clostridium cluster I, and Enterococcus were restored into normal level in the C. butyricum group, while only Bifidobacterium and Enterococcus reversed in the B. infantis group. Intriguingly, all predominant fecal bacteria reached normal level after the probiotic mixture treatment. Clostridium cluster XIVab, F. prausnitzii, Bifidobacterium, and Lactobacillus were significantly increased in the probiotic mixture group when compared with the C. butyricum group, while Bacteroides-Prevotella group, Clostridium cluster XIVab, Clostridium cluster XI, F. prausnitzii, Clostridium cluster I, and Lactobacillus were obviously higher in the probiotic mixture group than that in the B. infantis group. These observations suggested that the combined C. butyricum with B. infantis could restore the fecal microbiota more efficiently than the single probiotic strain.


Clostridium butyricum combined with Bifidobacterium infantis probiotic mixture restores fecal microbiota and attenuates systemic inflammation in mice with antibiotic-associated diarrhea.

Ling Z, Liu X, Cheng Y, Luo Y, Yuan L, Li L, Xiang C - Biomed Res Int (2015)

Bacterial loads in fecal microbiota as measured by qPCR (log10 copies/g fresh feces). The total bacteria were detected in the short-term and long-term treatment, while other predominant fecal microbiota were only detected in long-term treatment. Graph values are reported as the mean and standard deviation of the mean. Comparisons among the groups were calculated with Student's t-tests. P < 0.05 was labeled; ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352745&req=5

fig2: Bacterial loads in fecal microbiota as measured by qPCR (log10 copies/g fresh feces). The total bacteria were detected in the short-term and long-term treatment, while other predominant fecal microbiota were only detected in long-term treatment. Graph values are reported as the mean and standard deviation of the mean. Comparisons among the groups were calculated with Student's t-tests. P < 0.05 was labeled; ∗ compared with healthy control; # compared with AAD mice model; & compared with saline control; ∇ compared with C. butyricum treated group; ∙ compared with B. infantis treated group.
Mentions: The differences in predominant fecal bacteria after treatment with different probiotic strains and different terms were detected by qPCR (Figure 2). After 5-day treatment, the total bacteria in these treated groups could not reach healthy levels, with approximately one order of magnitude decrease when compared with the healthy mice. However, the total bacteria returned to normal level after 15-day treatment except the saline control group, which indicated that long-term administration of probiotic strains or probiotic mixture demonstrated a positive effect on modulating the intestinal microbiota in mice. In order to explore the specific modulation of the fecal microbiota, other nine predominant bacteria were detected after 15-day treatment. Our data demonstrated that Bacteroides-Prevotella group, Clostridium cluster XI, Clostridium cluster I, and Enterococcus were restored into normal level in the C. butyricum group, while only Bifidobacterium and Enterococcus reversed in the B. infantis group. Intriguingly, all predominant fecal bacteria reached normal level after the probiotic mixture treatment. Clostridium cluster XIVab, F. prausnitzii, Bifidobacterium, and Lactobacillus were significantly increased in the probiotic mixture group when compared with the C. butyricum group, while Bacteroides-Prevotella group, Clostridium cluster XIVab, Clostridium cluster XI, F. prausnitzii, Clostridium cluster I, and Lactobacillus were obviously higher in the probiotic mixture group than that in the B. infantis group. These observations suggested that the combined C. butyricum with B. infantis could restore the fecal microbiota more efficiently than the single probiotic strain.

Bottom Line: Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation.Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD.This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, China.

ABSTRACT
Antibiotic-associated diarrhea (AAD) is one of the most common complications of most types of antibiotics. Our aim was to determine the efficacy of Clostridium butyricum, Bifidobacterium infantis, and their mixture for AAD treatment in mice. AAD models were administered with single probiotic strain and probiotic mixture for short term and long term to evaluate the changes of the composition and diversity of intestinal microbiota, histopathology of the colon, and the systemic inflammation. Our data indicated that long-term probiotic therapy, but not short-term course, exerted beneficial effects on the restoration of the intestinal microbiota, the recovery of the tissue architecture, and attenuation of systemic inflammation. All predominant fecal bacteria reached normal level after the long-term probiotic mixture treatment, while IL-10, IFN-γ, and TNF-α also returned to normal level. However, the efficacy for AAD was time dependent and probiotic strain specific. Short-term administration of probiotic strains or mixture showed no apparent positive effects for AAD. In addition, the beneficial effects of C. butyricum combined with B. infantis probiotic mixture were superior to their single strain. This research showed that supplementation with C. butyricum combined with B. infantis probiotic mixture may be a simple and effective method for AAD treatment.

No MeSH data available.


Related in: MedlinePlus