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Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

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Related in: MedlinePlus

Radial strain and circumferential strain. (a)–(f) Radial strain anterior and inferior segments. (a)-(b) Radial strain apex, inferior and anterior segments per group. Strain in the anterior section of the LAD study is significantly lower during follow-up than at baseline. (c)-(d) Radial strain mid-ventricular level, inferior and anterior segments per group. (e)-(f) Radial strain basal level, inferior and anterior segments per group. (g)–(j) Circumferential strain. (g) Apical circumferential strain over time. (h) Circular strain at mid-ventricular level over time. (i) Circumferential strain at basal level over time. (j) Total ventricular circumferential strain over time. LCx indicates left circumflex artery. LAD: left anterior descending artery; BL: baseline. *P < 0.05 within the LAD group.
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fig4: Radial strain and circumferential strain. (a)–(f) Radial strain anterior and inferior segments. (a)-(b) Radial strain apex, inferior and anterior segments per group. Strain in the anterior section of the LAD study is significantly lower during follow-up than at baseline. (c)-(d) Radial strain mid-ventricular level, inferior and anterior segments per group. (e)-(f) Radial strain basal level, inferior and anterior segments per group. (g)–(j) Circumferential strain. (g) Apical circumferential strain over time. (h) Circular strain at mid-ventricular level over time. (i) Circumferential strain at basal level over time. (j) Total ventricular circumferential strain over time. LCx indicates left circumflex artery. LAD: left anterior descending artery; BL: baseline. *P < 0.05 within the LAD group.

Mentions: Decreased strain indicates deteriorated contractile function of the myocardium. In the LCx group, no changes in radial strain were observed in the infarcted inferior segment (Figure 4).


Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Radial strain and circumferential strain. (a)–(f) Radial strain anterior and inferior segments. (a)-(b) Radial strain apex, inferior and anterior segments per group. Strain in the anterior section of the LAD study is significantly lower during follow-up than at baseline. (c)-(d) Radial strain mid-ventricular level, inferior and anterior segments per group. (e)-(f) Radial strain basal level, inferior and anterior segments per group. (g)–(j) Circumferential strain. (g) Apical circumferential strain over time. (h) Circular strain at mid-ventricular level over time. (i) Circumferential strain at basal level over time. (j) Total ventricular circumferential strain over time. LCx indicates left circumflex artery. LAD: left anterior descending artery; BL: baseline. *P < 0.05 within the LAD group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352740&req=5

fig4: Radial strain and circumferential strain. (a)–(f) Radial strain anterior and inferior segments. (a)-(b) Radial strain apex, inferior and anterior segments per group. Strain in the anterior section of the LAD study is significantly lower during follow-up than at baseline. (c)-(d) Radial strain mid-ventricular level, inferior and anterior segments per group. (e)-(f) Radial strain basal level, inferior and anterior segments per group. (g)–(j) Circumferential strain. (g) Apical circumferential strain over time. (h) Circular strain at mid-ventricular level over time. (i) Circumferential strain at basal level over time. (j) Total ventricular circumferential strain over time. LCx indicates left circumflex artery. LAD: left anterior descending artery; BL: baseline. *P < 0.05 within the LAD group.
Mentions: Decreased strain indicates deteriorated contractile function of the myocardium. In the LCx group, no changes in radial strain were observed in the infarcted inferior segment (Figure 4).

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

Show MeSH
Related in: MedlinePlus