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Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

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Related in: MedlinePlus

Echocardiography. (a)–(c) LVEF and LV volumes on echocardiography over time. (a) Left ventricular ejection fraction (LVEF) over time. (b) Left ventricular end-diastolic volume (LVEDV) over time. (c) Left ventricular end-systolic volume (LVESV) over time. (d)–(f) 3D echocardiography at 12 weeks of FU. (d) LVEF; (e) LVEDV; and (f) LVESV at 12 weeks of FU as measured by 3D echocardiography. LCx indicates left circumflex artery. LAD: left anterior descending artery. (g) Example of echocardiogram at end of systole at mid-ventricular level at 12 weeks of FU. 1 indicates a formation of scar tissue at 12 week FU. Moreover, the end-systolic diameter has been increased. (h) Example of an echocardiogram of an animal in the LCx group at 12 weeks of FU at mid-ventricular level at the end of systole. 2 indicates the location of the scar. No clear myocardial thinning could be observed in the Lcx model. *P < 0.05.
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fig3: Echocardiography. (a)–(c) LVEF and LV volumes on echocardiography over time. (a) Left ventricular ejection fraction (LVEF) over time. (b) Left ventricular end-diastolic volume (LVEDV) over time. (c) Left ventricular end-systolic volume (LVESV) over time. (d)–(f) 3D echocardiography at 12 weeks of FU. (d) LVEF; (e) LVEDV; and (f) LVESV at 12 weeks of FU as measured by 3D echocardiography. LCx indicates left circumflex artery. LAD: left anterior descending artery. (g) Example of echocardiogram at end of systole at mid-ventricular level at 12 weeks of FU. 1 indicates a formation of scar tissue at 12 week FU. Moreover, the end-systolic diameter has been increased. (h) Example of an echocardiogram of an animal in the LCx group at 12 weeks of FU at mid-ventricular level at the end of systole. 2 indicates the location of the scar. No clear myocardial thinning could be observed in the Lcx model. *P < 0.05.

Mentions: Both LVEF and LV volumes before infarct induction were comparable between the LCx and LAD group. Post-AMI LVEF decreased with 5.9% to 50.8 ± 1.6% (P = 0.03) in the LCx group and with 17.1% to an average of 39.4 ± 1.6% in the LAD group (P = 0.009). Following AMI, LVESV increased with 5.6 ± 2.0 mL (P = NS) in the LCx group and with 17.8 ± 6.7 mL (P = 0.01) in the LAD group (Figure 3). There was no significant change in LVEDV following infarct induction in either of the groups. Table 1 depicts all cardiac dimensions in both groups.


Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Echocardiography. (a)–(c) LVEF and LV volumes on echocardiography over time. (a) Left ventricular ejection fraction (LVEF) over time. (b) Left ventricular end-diastolic volume (LVEDV) over time. (c) Left ventricular end-systolic volume (LVESV) over time. (d)–(f) 3D echocardiography at 12 weeks of FU. (d) LVEF; (e) LVEDV; and (f) LVESV at 12 weeks of FU as measured by 3D echocardiography. LCx indicates left circumflex artery. LAD: left anterior descending artery. (g) Example of echocardiogram at end of systole at mid-ventricular level at 12 weeks of FU. 1 indicates a formation of scar tissue at 12 week FU. Moreover, the end-systolic diameter has been increased. (h) Example of an echocardiogram of an animal in the LCx group at 12 weeks of FU at mid-ventricular level at the end of systole. 2 indicates the location of the scar. No clear myocardial thinning could be observed in the Lcx model. *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4352740&req=5

fig3: Echocardiography. (a)–(c) LVEF and LV volumes on echocardiography over time. (a) Left ventricular ejection fraction (LVEF) over time. (b) Left ventricular end-diastolic volume (LVEDV) over time. (c) Left ventricular end-systolic volume (LVESV) over time. (d)–(f) 3D echocardiography at 12 weeks of FU. (d) LVEF; (e) LVEDV; and (f) LVESV at 12 weeks of FU as measured by 3D echocardiography. LCx indicates left circumflex artery. LAD: left anterior descending artery. (g) Example of echocardiogram at end of systole at mid-ventricular level at 12 weeks of FU. 1 indicates a formation of scar tissue at 12 week FU. Moreover, the end-systolic diameter has been increased. (h) Example of an echocardiogram of an animal in the LCx group at 12 weeks of FU at mid-ventricular level at the end of systole. 2 indicates the location of the scar. No clear myocardial thinning could be observed in the Lcx model. *P < 0.05.
Mentions: Both LVEF and LV volumes before infarct induction were comparable between the LCx and LAD group. Post-AMI LVEF decreased with 5.9% to 50.8 ± 1.6% (P = 0.03) in the LCx group and with 17.1% to an average of 39.4 ± 1.6% in the LAD group (P = 0.009). Following AMI, LVESV increased with 5.6 ± 2.0 mL (P = NS) in the LCx group and with 17.8 ± 6.7 mL (P = 0.01) in the LAD group (Figure 3). There was no significant change in LVEDV following infarct induction in either of the groups. Table 1 depicts all cardiac dimensions in both groups.

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

Show MeSH
Related in: MedlinePlus