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Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

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Related in: MedlinePlus

Schematic overview of strain and 17-segment echocardiography model. (a) Schematic overview of strain analysis. Radial strain represents thickening (systole, arrows outwards) and thinning (diastole, arrows facing each other) of the myocardium during 1 cardiac cycle. Circumferential strain: elongation and shortening (arrows facing each other) of myocardial muscle fibers. (b) Schematic overview of myocardial segments on short axis view at basal level (mitral valve level). A indicates anterior wall. AL: anterolateral wall; IL: inferolateral; I: inferior; IS: inferoseptal; AS: anteroseptal. (c) Schematic overview of short axis view of myocardial segments at mid-ventricular level (papillary muscle level). (d) Schematic view of the apex. The apex only consists of 4 segments. L indicates lateral wall.
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fig2: Schematic overview of strain and 17-segment echocardiography model. (a) Schematic overview of strain analysis. Radial strain represents thickening (systole, arrows outwards) and thinning (diastole, arrows facing each other) of the myocardium during 1 cardiac cycle. Circumferential strain: elongation and shortening (arrows facing each other) of myocardial muscle fibers. (b) Schematic overview of myocardial segments on short axis view at basal level (mitral valve level). A indicates anterior wall. AL: anterolateral wall; IL: inferolateral; I: inferior; IS: inferoseptal; AS: anteroseptal. (c) Schematic overview of short axis view of myocardial segments at mid-ventricular level (papillary muscle level). (d) Schematic view of the apex. The apex only consists of 4 segments. L indicates lateral wall.

Mentions: Strain is defined as the total deformation of the myocardium during 1 cardiac cycle [25, 26]. Radial and circumferential strains were analyzed on the 2D echocardiography short axis views via speckle tracking (VVI, Siemens Medical solutions, USA) as previously described [25]. Strain was analyzed according to the 17-segment echocardiography model. Figure 2 provides a schematic overview of the radial strain and circumferential strain and the 17-segment echocardiography model. In the LAD model anterior and anteroseptal segments were analyzed, whereas the inferior and inferolateral segments were used as reference segments in this group. In the LCx group, inferior and inferolateral segments were mostly affected and anterior segments were used as reference segments. Moreover, global strain was calculated by the software. Strain is represented as percentage of left ventricular deformation.


Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Schematic overview of strain and 17-segment echocardiography model. (a) Schematic overview of strain analysis. Radial strain represents thickening (systole, arrows outwards) and thinning (diastole, arrows facing each other) of the myocardium during 1 cardiac cycle. Circumferential strain: elongation and shortening (arrows facing each other) of myocardial muscle fibers. (b) Schematic overview of myocardial segments on short axis view at basal level (mitral valve level). A indicates anterior wall. AL: anterolateral wall; IL: inferolateral; I: inferior; IS: inferoseptal; AS: anteroseptal. (c) Schematic overview of short axis view of myocardial segments at mid-ventricular level (papillary muscle level). (d) Schematic view of the apex. The apex only consists of 4 segments. L indicates lateral wall.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352740&req=5

fig2: Schematic overview of strain and 17-segment echocardiography model. (a) Schematic overview of strain analysis. Radial strain represents thickening (systole, arrows outwards) and thinning (diastole, arrows facing each other) of the myocardium during 1 cardiac cycle. Circumferential strain: elongation and shortening (arrows facing each other) of myocardial muscle fibers. (b) Schematic overview of myocardial segments on short axis view at basal level (mitral valve level). A indicates anterior wall. AL: anterolateral wall; IL: inferolateral; I: inferior; IS: inferoseptal; AS: anteroseptal. (c) Schematic overview of short axis view of myocardial segments at mid-ventricular level (papillary muscle level). (d) Schematic view of the apex. The apex only consists of 4 segments. L indicates lateral wall.
Mentions: Strain is defined as the total deformation of the myocardium during 1 cardiac cycle [25, 26]. Radial and circumferential strains were analyzed on the 2D echocardiography short axis views via speckle tracking (VVI, Siemens Medical solutions, USA) as previously described [25]. Strain was analyzed according to the 17-segment echocardiography model. Figure 2 provides a schematic overview of the radial strain and circumferential strain and the 17-segment echocardiography model. In the LAD model anterior and anteroseptal segments were analyzed, whereas the inferior and inferolateral segments were used as reference segments in this group. In the LCx group, inferior and inferolateral segments were mostly affected and anterior segments were used as reference segments. Moreover, global strain was calculated by the software. Strain is represented as percentage of left ventricular deformation.

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

Show MeSH
Related in: MedlinePlus