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Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

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Related in: MedlinePlus

Study design. (a) Flowchart. (b) 1: left anterior descending artery (LAD); 2: ligation of the LAD was located after the first diagonal branch; 3: location and example of balloon occlusion of the left circumflex artery (LCx). BL: baseline before infarct induction; PMI: postmyocardial infarct; w: week.
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Related In: Results  -  Collection


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fig1: Study design. (a) Flowchart. (b) 1: left anterior descending artery (LAD); 2: ligation of the LAD was located after the first diagonal branch; 3: location and example of balloon occlusion of the left circumflex artery (LCx). BL: baseline before infarct induction; PMI: postmyocardial infarct; w: week.

Mentions: An 8F sheath was inserted in the carotid artery and an 8F guiding catheter (JL 3.5–4.0, Boston Scientific Nederland BV, Nieuwegein, The Netherlands) was positioned at the ostium of the left main coronary artery. An angioplasty balloon (Trek 3.5–4.0 ×12, Abbott) was inflated (8–14 bar) for 150 min in the proximal LCx (Figure 1). After balloon inflation, the guiding catheter was carefully retracted to enable normal blood flow through the nonoccluded part of the left coronary system. Position was verified every 15 minutes to ensure appropriate occlusion of the LCx. After the procedure catheters were removed and the wound was closed.


Cardiac function in a long-term follow-up study of moderate and severe porcine model of chronic myocardial infarction.

de Jong R, van Hout GP, Houtgraaf JH, Takashima S, Pasterkamp G, Hoefer I, Duckers HJ - Biomed Res Int (2015)

Study design. (a) Flowchart. (b) 1: left anterior descending artery (LAD); 2: ligation of the LAD was located after the first diagonal branch; 3: location and example of balloon occlusion of the left circumflex artery (LCx). BL: baseline before infarct induction; PMI: postmyocardial infarct; w: week.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352740&req=5

fig1: Study design. (a) Flowchart. (b) 1: left anterior descending artery (LAD); 2: ligation of the LAD was located after the first diagonal branch; 3: location and example of balloon occlusion of the left circumflex artery (LCx). BL: baseline before infarct induction; PMI: postmyocardial infarct; w: week.
Mentions: An 8F sheath was inserted in the carotid artery and an 8F guiding catheter (JL 3.5–4.0, Boston Scientific Nederland BV, Nieuwegein, The Netherlands) was positioned at the ostium of the left main coronary artery. An angioplasty balloon (Trek 3.5–4.0 ×12, Abbott) was inflated (8–14 bar) for 150 min in the proximal LCx (Figure 1). After balloon inflation, the guiding catheter was carefully retracted to enable normal blood flow through the nonoccluded part of the left coronary system. Position was verified every 15 minutes to ensure appropriate occlusion of the LCx. After the procedure catheters were removed and the wound was closed.

Bottom Line: At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

View Article: PubMed Central - PubMed

Affiliation: Molecular Cardiology Laboratory, Erasmus University Medical Center, Room 2389a, P.O. Box 2040, 3000 CA Rotterdam, Netherlands.

ABSTRACT

Background: Novel therapies need to be evaluated in a relevant large animal model that mimics the clinical course and treatment in a reasonable time frame. To reliably assess therapeutic efficacy, knowledge regarding the translational model and the course of disease is needed.

Methods: Landrace pigs were subjected to a transient occlusion of the proximal left circumflex artery (LCx) (n = 6) or mid-left anterior descending artery (LAD) (n = 6) for 150 min. Cardiac function was evaluated before by 2D echocardiography or 3D echocardiography and pressure-volume loop analysis. At 12 weeks of follow-up the heart was excised for histological analysis and infarct size calculations.

Results: Directly following AMI, LVEF was severely reduced compared to baseline in the LAD group (-17.1 ± 1.6%, P = 0.009) compared to only a moderate reduction in the LCx group (-5.9 ± 1.5%, P = 0.02) and this effect remained unchanged during 12 weeks of follow-up.

Conclusion: Two models of chronic MI, representative for different patient groups, can reproducibly be created through clinically relevant ischemia-reperfusion of the mid-LAD and proximal LCx.

Show MeSH
Related in: MedlinePlus