Limits...
G protein-coupled receptors in stem cell maintenance and somatic reprogramming to pluripotent or cancer stem cells.

Choi HY, Saha SK, Kim K, Kim S, Yang GM, Kim B, Kim JH, Cho SG - BMB Rep (2015)

Bottom Line: Several studies have also confirmed their central roles in embryonic development and stem cell maintenance.Our study and other reports have revealed that the expression of many GPCRs is modulated during the generation of induced PSCs (iPSCs) or CSCs as well as during CSC sphere formation.This review addresses the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PSCs or CSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Seoul 143-701, Korea.

ABSTRACT
G protein-coupled receptors (GPCRs) are a large class of transmembrane receptors categorized into five distinct families: rhodopsin, secretin, adhesion, glutamate, and frizzled. They bind and regulate 80% of all hormones and account for 20-50% of the pharmaceuticals currently on the market. Hundreds of GPCRs integrate and coordinate the functions of individual cells, mediating signaling between various organs. GPCRs are crucial players in tumor progression, adipogenesis, and inflammation. Several studies have also confirmed their central roles in embryonic development and stem cell maintenance. Recently, GPCRs have emerged as key players in the regulation of cell survival, proliferation, migration, and self-renewal in pluripotent (PSCs) and cancer stem cells (CSCs). Our study and other reports have revealed that the expression of many GPCRs is modulated during the generation of induced PSCs (iPSCs) or CSCs as well as during CSC sphere formation. These GPCRs may have crucial roles in the regulation of selfrenewal and other biological properties of iPSCs and CSCs. This review addresses the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PSCs or CSCs.

Show MeSH

Related in: MedlinePlus

Changes in G protein-coupled receptor (GPCR) expression in stem cell maintenance and/or during somatic reprogramming to iPSCs or CSCs. The transcriptional profile of the selected GPCR family was analyzed using high-throughput RNA sequencing. (A) GPCRs showing up- or down-regulated expression during stem cell maintenance. (B) GPCRs showing up- or down-regulated expression during somatic reprogramming to iPSCs. (C) GPCRs showing up- or down-regulated expression during CSC sphere formation. (D) GPCRs showing up- or down-regulated expression during malignant transformation (somatic reprogramming) to CSCs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4352616&req=5

Figure 001: Changes in G protein-coupled receptor (GPCR) expression in stem cell maintenance and/or during somatic reprogramming to iPSCs or CSCs. The transcriptional profile of the selected GPCR family was analyzed using high-throughput RNA sequencing. (A) GPCRs showing up- or down-regulated expression during stem cell maintenance. (B) GPCRs showing up- or down-regulated expression during somatic reprogramming to iPSCs. (C) GPCRs showing up- or down-regulated expression during CSC sphere formation. (D) GPCRs showing up- or down-regulated expression during malignant transformation (somatic reprogramming) to CSCs.

Mentions: Recently, we analyzed the expression profiles of GPCRs during somatic reprogramming to iPSCs or CSCs and during CSC sphere formation (Fig. 1 and Table 1). More than 106 GPCRs were over-expressed in the PCSs or CSCs, whereas the expression of 22 GPCRs was down-regulated during somatic reprogramming to iPSCs. Eighty-one GPCRs were differentially expressed during somatic reprogramming to iPSCs, and the expression of 195 GPCRs was either up- or down-regulated during somatic reprogramming to CSCs and sphere formation of CSCs. These data suggest that various GPCRs may have key roles in somatic reprogramming to iPSCs or CSCs and may be involved in the regulation of self-renewal and other biological properties of PCSs or CSCs. Recently, much evidence has accumulated supporting the specific roles of GPCRs in somatic reprogramming or transformation to iPSCs or CSCs. In the following section, we review the general role of GPCR signaling pathways and the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PCSs or CSCs.


G protein-coupled receptors in stem cell maintenance and somatic reprogramming to pluripotent or cancer stem cells.

Choi HY, Saha SK, Kim K, Kim S, Yang GM, Kim B, Kim JH, Cho SG - BMB Rep (2015)

Changes in G protein-coupled receptor (GPCR) expression in stem cell maintenance and/or during somatic reprogramming to iPSCs or CSCs. The transcriptional profile of the selected GPCR family was analyzed using high-throughput RNA sequencing. (A) GPCRs showing up- or down-regulated expression during stem cell maintenance. (B) GPCRs showing up- or down-regulated expression during somatic reprogramming to iPSCs. (C) GPCRs showing up- or down-regulated expression during CSC sphere formation. (D) GPCRs showing up- or down-regulated expression during malignant transformation (somatic reprogramming) to CSCs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4352616&req=5

Figure 001: Changes in G protein-coupled receptor (GPCR) expression in stem cell maintenance and/or during somatic reprogramming to iPSCs or CSCs. The transcriptional profile of the selected GPCR family was analyzed using high-throughput RNA sequencing. (A) GPCRs showing up- or down-regulated expression during stem cell maintenance. (B) GPCRs showing up- or down-regulated expression during somatic reprogramming to iPSCs. (C) GPCRs showing up- or down-regulated expression during CSC sphere formation. (D) GPCRs showing up- or down-regulated expression during malignant transformation (somatic reprogramming) to CSCs.
Mentions: Recently, we analyzed the expression profiles of GPCRs during somatic reprogramming to iPSCs or CSCs and during CSC sphere formation (Fig. 1 and Table 1). More than 106 GPCRs were over-expressed in the PCSs or CSCs, whereas the expression of 22 GPCRs was down-regulated during somatic reprogramming to iPSCs. Eighty-one GPCRs were differentially expressed during somatic reprogramming to iPSCs, and the expression of 195 GPCRs was either up- or down-regulated during somatic reprogramming to CSCs and sphere formation of CSCs. These data suggest that various GPCRs may have key roles in somatic reprogramming to iPSCs or CSCs and may be involved in the regulation of self-renewal and other biological properties of PCSs or CSCs. Recently, much evidence has accumulated supporting the specific roles of GPCRs in somatic reprogramming or transformation to iPSCs or CSCs. In the following section, we review the general role of GPCR signaling pathways and the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PCSs or CSCs.

Bottom Line: Several studies have also confirmed their central roles in embryonic development and stem cell maintenance.Our study and other reports have revealed that the expression of many GPCRs is modulated during the generation of induced PSCs (iPSCs) or CSCs as well as during CSC sphere formation.This review addresses the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PSCs or CSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Biotechnology, Animal Resources Research Center, and Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Seoul 143-701, Korea.

ABSTRACT
G protein-coupled receptors (GPCRs) are a large class of transmembrane receptors categorized into five distinct families: rhodopsin, secretin, adhesion, glutamate, and frizzled. They bind and regulate 80% of all hormones and account for 20-50% of the pharmaceuticals currently on the market. Hundreds of GPCRs integrate and coordinate the functions of individual cells, mediating signaling between various organs. GPCRs are crucial players in tumor progression, adipogenesis, and inflammation. Several studies have also confirmed their central roles in embryonic development and stem cell maintenance. Recently, GPCRs have emerged as key players in the regulation of cell survival, proliferation, migration, and self-renewal in pluripotent (PSCs) and cancer stem cells (CSCs). Our study and other reports have revealed that the expression of many GPCRs is modulated during the generation of induced PSCs (iPSCs) or CSCs as well as during CSC sphere formation. These GPCRs may have crucial roles in the regulation of selfrenewal and other biological properties of iPSCs and CSCs. This review addresses the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PSCs or CSCs.

Show MeSH
Related in: MedlinePlus