Limits...
Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products.

Song MJ, Lee JJ, Nam YH, Kim TG, Chung YW, Kim M, Choi YE, Shin MH, Kim HP - BMB Rep (2015)

Bottom Line: In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells.Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10.The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.

ABSTRACT
Trichomoniasis caused by the parasitic protozoan Trichomonas vaginalis is the most common sexually transmitted disease in the world. Dendritic cells are antigen presenting cells that initiate immune responses by directing the activation and differentiation of naïve T cells. In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells. Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10. The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells. Chromatin immunoprecipitation assay demonstrated that IL-12 expression was regulated at the chromatin level in T. vaginalis-derived Secretory Productstreated dendritic cells. Our results demonstrated that T. vaginalis- derived Secretory Products modulate the maturation and cytokine production of dendritic cells leading to immune tolerance.

Show MeSH

Related in: MedlinePlus

The DC-modulatory activity of TvSP is abolished by treatment with Proteinase K. DCs were differentiated with GM-CSF from BM precursors in the presence of mock-treated or Proteinase K-treated TvSP. (A-D) On day 10, DCs were treated with 100 ng/ml LPS for 24 h to induce maturation, and expression of MHC-II (A), CD40 (B), CD80 (C) and CD86 (D) was analyzed by flow cytometry. Isotype controls are shown as shaded histograms, control BMDCs are shown as red lines, and TvSP-treated BMDCs are shown as blue lines. Mean fluorescent intensity (MFI) values are indicated with corresponding colors. Results are representative of more than three independent experiments. (E, F) Production of cytokines IL-12 (E) and IL-10 (F) induced by LPS (100 ng/ml) treatment of BMDCs for 24 h as measured by cytometric bead array. All data are expressed as the means ± SD (n = 3), and statistical significance (*P < 0.05) is shown for treatments compared with the controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4352611&req=5

Figure 003: The DC-modulatory activity of TvSP is abolished by treatment with Proteinase K. DCs were differentiated with GM-CSF from BM precursors in the presence of mock-treated or Proteinase K-treated TvSP. (A-D) On day 10, DCs were treated with 100 ng/ml LPS for 24 h to induce maturation, and expression of MHC-II (A), CD40 (B), CD80 (C) and CD86 (D) was analyzed by flow cytometry. Isotype controls are shown as shaded histograms, control BMDCs are shown as red lines, and TvSP-treated BMDCs are shown as blue lines. Mean fluorescent intensity (MFI) values are indicated with corresponding colors. Results are representative of more than three independent experiments. (E, F) Production of cytokines IL-12 (E) and IL-10 (F) induced by LPS (100 ng/ml) treatment of BMDCs for 24 h as measured by cytometric bead array. All data are expressed as the means ± SD (n = 3), and statistical significance (*P < 0.05) is shown for treatments compared with the controls.

Mentions: To understand the components of TvSP that are responsible for the regulation of surface molecule expression and cytokine production, we treated TvSP with Proteinase K and examined the effects on BMDCs. Compared to the mock-treated TvSP, which down-regulated expression of MHC-II, CD40, CD80, and CD86 especially when BMDCs were treated with LPS, treatment of TvSP with Proteinase K abrogated its inhibitory activity on the expression of these surface molecules (Fig. 3A-3D). The down-regulation of IL-12 production and up-regulation of IL-10 expression by TvSP also were abolished by Proteinase K treatment (Fig. 3E and 3F). These results show that a proteinaceous component of TvSP was responsible for the modulatory functions of BMDCs during differentiation with GM-CSF.


Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products.

Song MJ, Lee JJ, Nam YH, Kim TG, Chung YW, Kim M, Choi YE, Shin MH, Kim HP - BMB Rep (2015)

The DC-modulatory activity of TvSP is abolished by treatment with Proteinase K. DCs were differentiated with GM-CSF from BM precursors in the presence of mock-treated or Proteinase K-treated TvSP. (A-D) On day 10, DCs were treated with 100 ng/ml LPS for 24 h to induce maturation, and expression of MHC-II (A), CD40 (B), CD80 (C) and CD86 (D) was analyzed by flow cytometry. Isotype controls are shown as shaded histograms, control BMDCs are shown as red lines, and TvSP-treated BMDCs are shown as blue lines. Mean fluorescent intensity (MFI) values are indicated with corresponding colors. Results are representative of more than three independent experiments. (E, F) Production of cytokines IL-12 (E) and IL-10 (F) induced by LPS (100 ng/ml) treatment of BMDCs for 24 h as measured by cytometric bead array. All data are expressed as the means ± SD (n = 3), and statistical significance (*P < 0.05) is shown for treatments compared with the controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4352611&req=5

Figure 003: The DC-modulatory activity of TvSP is abolished by treatment with Proteinase K. DCs were differentiated with GM-CSF from BM precursors in the presence of mock-treated or Proteinase K-treated TvSP. (A-D) On day 10, DCs were treated with 100 ng/ml LPS for 24 h to induce maturation, and expression of MHC-II (A), CD40 (B), CD80 (C) and CD86 (D) was analyzed by flow cytometry. Isotype controls are shown as shaded histograms, control BMDCs are shown as red lines, and TvSP-treated BMDCs are shown as blue lines. Mean fluorescent intensity (MFI) values are indicated with corresponding colors. Results are representative of more than three independent experiments. (E, F) Production of cytokines IL-12 (E) and IL-10 (F) induced by LPS (100 ng/ml) treatment of BMDCs for 24 h as measured by cytometric bead array. All data are expressed as the means ± SD (n = 3), and statistical significance (*P < 0.05) is shown for treatments compared with the controls.
Mentions: To understand the components of TvSP that are responsible for the regulation of surface molecule expression and cytokine production, we treated TvSP with Proteinase K and examined the effects on BMDCs. Compared to the mock-treated TvSP, which down-regulated expression of MHC-II, CD40, CD80, and CD86 especially when BMDCs were treated with LPS, treatment of TvSP with Proteinase K abrogated its inhibitory activity on the expression of these surface molecules (Fig. 3A-3D). The down-regulation of IL-12 production and up-regulation of IL-10 expression by TvSP also were abolished by Proteinase K treatment (Fig. 3E and 3F). These results show that a proteinaceous component of TvSP was responsible for the modulatory functions of BMDCs during differentiation with GM-CSF.

Bottom Line: In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells.Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10.The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.

ABSTRACT
Trichomoniasis caused by the parasitic protozoan Trichomonas vaginalis is the most common sexually transmitted disease in the world. Dendritic cells are antigen presenting cells that initiate immune responses by directing the activation and differentiation of naïve T cells. In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells. Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10. The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells. Chromatin immunoprecipitation assay demonstrated that IL-12 expression was regulated at the chromatin level in T. vaginalis-derived Secretory Productstreated dendritic cells. Our results demonstrated that T. vaginalis- derived Secretory Products modulate the maturation and cytokine production of dendritic cells leading to immune tolerance.

Show MeSH
Related in: MedlinePlus