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Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products.

Song MJ, Lee JJ, Nam YH, Kim TG, Chung YW, Kim M, Choi YE, Shin MH, Kim HP - BMB Rep (2015)

Bottom Line: In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells.Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10.The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.

ABSTRACT
Trichomoniasis caused by the parasitic protozoan Trichomonas vaginalis is the most common sexually transmitted disease in the world. Dendritic cells are antigen presenting cells that initiate immune responses by directing the activation and differentiation of naïve T cells. In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells. Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10. The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells. Chromatin immunoprecipitation assay demonstrated that IL-12 expression was regulated at the chromatin level in T. vaginalis-derived Secretory Productstreated dendritic cells. Our results demonstrated that T. vaginalis- derived Secretory Products modulate the maturation and cytokine production of dendritic cells leading to immune tolerance.

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The effect of TvSP on the cytokine production of BMDC. DCs were differentiated with GM-CSF from BM precursors in the absence or presence of TvSP. (A-E) Immature DCs were stimulated with 100 ng/ml LPS for 6 h, and the mRNA levels of Il12a (A), Il12b (B), Il10 (C), Tnf (D), and Il6 (E) were measured by qRT-PCR. (F-I) Immature DCs were stimulated with 100 ng/ml LPS for 24 h, and the expression of IL-12 (F), IL-10 (G), TNF-α (H), and IL-6 (I) was measured by Cytometric Bead Array. All data are expressed as mean ± SD, and statistical significance (*P < 0.05, or **P < 0.01) is shown for treatments compared with the controls.
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Figure 002: The effect of TvSP on the cytokine production of BMDC. DCs were differentiated with GM-CSF from BM precursors in the absence or presence of TvSP. (A-E) Immature DCs were stimulated with 100 ng/ml LPS for 6 h, and the mRNA levels of Il12a (A), Il12b (B), Il10 (C), Tnf (D), and Il6 (E) were measured by qRT-PCR. (F-I) Immature DCs were stimulated with 100 ng/ml LPS for 24 h, and the expression of IL-12 (F), IL-10 (G), TNF-α (H), and IL-6 (I) was measured by Cytometric Bead Array. All data are expressed as mean ± SD, and statistical significance (*P < 0.05, or **P < 0.01) is shown for treatments compared with the controls.

Mentions: In order to examine whether TvSP may affect the cytokine production capacity of BMDCs during DC differentiation, we performed quantitative real-time Polymerase Chain Reaction (qRT-PCR) using BMDCs that were differentiated in the absence or presence of TvSP for 10 days and then stimulated with LPS. This analysis demonstrated that the mRNA levels of Il12a, Il12b, Il10, Tnf, and Il6 were markedly increased by LPS stimulation in control BMDCs, consistent with a previous report (2). Interestingly, the induction of Il12a and Il12b mRNA expression in response to LPS was significantly lower in BMDCs differentiated in the presence of TvSP compared to the BMDCs differentiated in the absence of TvSP (Fig. 2A and 2B). In contrast, differentiation of BMDCs in the presence of TvSP resulted in higher expression of the immunosuppressive cytokine Il10 compared to the control BMDCs (Fig. 2C). TvSP exerted a minimal effect on the mRNA expression of other proinflammatory cytokines such as Tnf and Il6 (Fig. 2D and 2E).


Modulation of dendritic cell function by Trichomonas vaginalis-derived secretory products.

Song MJ, Lee JJ, Nam YH, Kim TG, Chung YW, Kim M, Choi YE, Shin MH, Kim HP - BMB Rep (2015)

The effect of TvSP on the cytokine production of BMDC. DCs were differentiated with GM-CSF from BM precursors in the absence or presence of TvSP. (A-E) Immature DCs were stimulated with 100 ng/ml LPS for 6 h, and the mRNA levels of Il12a (A), Il12b (B), Il10 (C), Tnf (D), and Il6 (E) were measured by qRT-PCR. (F-I) Immature DCs were stimulated with 100 ng/ml LPS for 24 h, and the expression of IL-12 (F), IL-10 (G), TNF-α (H), and IL-6 (I) was measured by Cytometric Bead Array. All data are expressed as mean ± SD, and statistical significance (*P < 0.05, or **P < 0.01) is shown for treatments compared with the controls.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4352611&req=5

Figure 002: The effect of TvSP on the cytokine production of BMDC. DCs were differentiated with GM-CSF from BM precursors in the absence or presence of TvSP. (A-E) Immature DCs were stimulated with 100 ng/ml LPS for 6 h, and the mRNA levels of Il12a (A), Il12b (B), Il10 (C), Tnf (D), and Il6 (E) were measured by qRT-PCR. (F-I) Immature DCs were stimulated with 100 ng/ml LPS for 24 h, and the expression of IL-12 (F), IL-10 (G), TNF-α (H), and IL-6 (I) was measured by Cytometric Bead Array. All data are expressed as mean ± SD, and statistical significance (*P < 0.05, or **P < 0.01) is shown for treatments compared with the controls.
Mentions: In order to examine whether TvSP may affect the cytokine production capacity of BMDCs during DC differentiation, we performed quantitative real-time Polymerase Chain Reaction (qRT-PCR) using BMDCs that were differentiated in the absence or presence of TvSP for 10 days and then stimulated with LPS. This analysis demonstrated that the mRNA levels of Il12a, Il12b, Il10, Tnf, and Il6 were markedly increased by LPS stimulation in control BMDCs, consistent with a previous report (2). Interestingly, the induction of Il12a and Il12b mRNA expression in response to LPS was significantly lower in BMDCs differentiated in the presence of TvSP compared to the BMDCs differentiated in the absence of TvSP (Fig. 2A and 2B). In contrast, differentiation of BMDCs in the presence of TvSP resulted in higher expression of the immunosuppressive cytokine Il10 compared to the control BMDCs (Fig. 2C). TvSP exerted a minimal effect on the mRNA expression of other proinflammatory cytokines such as Tnf and Il6 (Fig. 2D and 2E).

Bottom Line: In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells.Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10.The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.

ABSTRACT
Trichomoniasis caused by the parasitic protozoan Trichomonas vaginalis is the most common sexually transmitted disease in the world. Dendritic cells are antigen presenting cells that initiate immune responses by directing the activation and differentiation of naïve T cells. In this study, we analyzed the effect of Trichomonas vaginalis-derived Secretory Products on the differentiation and function of dendritic cells. Differentiation of bone marrow-derived dendritic cells in the presence of T. vaginalis-derived Secretory Products resulted in inhibition of lipopolysaccharide-induced maturation of dendritic cells, down-regulation of IL-12, and up-regulation of IL-10. The protein components of T. vaginalis-derived Secretory Products were shown to be responsible for altered function of bone marrow- derived dendritic cells. Chromatin immunoprecipitation assay demonstrated that IL-12 expression was regulated at the chromatin level in T. vaginalis-derived Secretory Productstreated dendritic cells. Our results demonstrated that T. vaginalis- derived Secretory Products modulate the maturation and cytokine production of dendritic cells leading to immune tolerance.

Show MeSH
Related in: MedlinePlus