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Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China.

Zhou W, Niu D, Cao X, Ning M, Zhang Z, Shen H, Zhang K - BMC Infect. Dis. (2015)

Bottom Line: The MICs of linezolid on these 5 isolates were >256 μg/mL.The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains.The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. zwq_096@163.com.

ABSTRACT

Background: The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital.

Methods: The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE).

Results: The MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates.

Conclusions: The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.

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Related in: MedlinePlus

PFGE profiles ofStaphylococcus capitisobtained withSma-I. M, λ molecular marker; Line 1, SA10106; Line 2, SA13096; Line 3, SA23062; Line 4, SA20062; Line 5, SA11026; Line 6, SA01; Line 7,SA02; Line 8, SA03; Line 9, SA04.
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Fig1: PFGE profiles ofStaphylococcus capitisobtained withSma-I. M, λ molecular marker; Line 1, SA10106; Line 2, SA13096; Line 3, SA23062; Line 4, SA20062; Line 5, SA11026; Line 6, SA01; Line 7,SA02; Line 8, SA03; Line 9, SA04.

Mentions: PCR mapping and sequencing results showed that G2576T, the most common mutation in domain V of the 23S rRNA gene [10], was not be detected in all of the 5 LRSC isolates. However, a new mutation, G2603T, which had not been previously reported in S. capitis, was observed in these 5 isolates with the cfr gene being simultaneously prevalent among them (Table 2), while none of the 4 LSSC isolates carried cfr gene and had mutations in the 23S rRNA. Moreover, the mutations in the L3, L4 or L22 were not detected among LRSC isolates in our current study. The genetic relationship between these LRSC strains (Figure 1) revealed a clonal dissemination of LRSC isolates as displayed by the identical Sma-I digestion patterns.Figure 1


Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China.

Zhou W, Niu D, Cao X, Ning M, Zhang Z, Shen H, Zhang K - BMC Infect. Dis. (2015)

PFGE profiles ofStaphylococcus capitisobtained withSma-I. M, λ molecular marker; Line 1, SA10106; Line 2, SA13096; Line 3, SA23062; Line 4, SA20062; Line 5, SA11026; Line 6, SA01; Line 7,SA02; Line 8, SA03; Line 9, SA04.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4352562&req=5

Fig1: PFGE profiles ofStaphylococcus capitisobtained withSma-I. M, λ molecular marker; Line 1, SA10106; Line 2, SA13096; Line 3, SA23062; Line 4, SA20062; Line 5, SA11026; Line 6, SA01; Line 7,SA02; Line 8, SA03; Line 9, SA04.
Mentions: PCR mapping and sequencing results showed that G2576T, the most common mutation in domain V of the 23S rRNA gene [10], was not be detected in all of the 5 LRSC isolates. However, a new mutation, G2603T, which had not been previously reported in S. capitis, was observed in these 5 isolates with the cfr gene being simultaneously prevalent among them (Table 2), while none of the 4 LSSC isolates carried cfr gene and had mutations in the 23S rRNA. Moreover, the mutations in the L3, L4 or L22 were not detected among LRSC isolates in our current study. The genetic relationship between these LRSC strains (Figure 1) revealed a clonal dissemination of LRSC isolates as displayed by the identical Sma-I digestion patterns.Figure 1

Bottom Line: The MICs of linezolid on these 5 isolates were >256 μg/mL.The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains.The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing, Jiangsu Province, 210008, PR China. zwq_096@163.com.

ABSTRACT

Background: The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital.

Methods: The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE).

Results: The MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates.

Conclusions: The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.

Show MeSH
Related in: MedlinePlus