Limits...
Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital.

Villalón P, Valdezate S, Cabezas T, Ortega M, Garrido N, Vindel A, Medina-Pascual MJ, Saez-Nieto JA - BMC Microbiol. (2015)

Bottom Line: MLVA-8Orsay returned 17 allelic profiles.MLST and the VNTR L-markers grouped the isolates into clonal clusters.The wide diversity of MLVA small (S)-markers, however, did not permit clustering.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda-Pozuelo km2, 28220, Madrid, Majadahonda, Spain. pvillalon@isciii.es.

ABSTRACT

Background: Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period.

Results: PFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla OXA-58-like and bla OXA-40-like genes was 60% and 40% respectively.

Conclusions: PFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80.

Show MeSH

Related in: MedlinePlus

Distribution of endemic and epidemicAcinetobacter baumanniiPFGE types. The top chart shows the temporal distribution of the 15 endemic and epidemic PFGE types, and the sequence types (ST). Vertical arrows indicate the nosocomial outbreaks. The bottom table shows the detailed data of the temporal and spatial distribution of the PFGE types. Abreviations: 2 F, second floor; 3 F, third floor; 4 F, fourth floor; 5 F, fifth floor; ICU, intensive care unit; SR, surgery room; ER, emergency room; OC; out-patient clinic consultation room.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4352537&req=5

Fig2: Distribution of endemic and epidemicAcinetobacter baumanniiPFGE types. The top chart shows the temporal distribution of the 15 endemic and epidemic PFGE types, and the sequence types (ST). Vertical arrows indicate the nosocomial outbreaks. The bottom table shows the detailed data of the temporal and spatial distribution of the PFGE types. Abreviations: 2 F, second floor; 3 F, third floor; 4 F, fourth floor; 5 F, fifth floor; ICU, intensive care unit; SR, surgery room; ER, emergency room; OC; out-patient clinic consultation room.

Mentions: Figure 1 shows the PFGE genetic similarity dendogram. The PFGE types were numbered EE1 to EE15. The Hunter-Gaston diversity index (HGDI) [14] calculated for the PFGE technique with respect to the further-examined 231 isolates was 0.880 (Table 1). The genetic similarity range for their 15 PFGE types was 60.4 – 100%. Five clusters (75% cut-off) were observed; their genetic similarity values were EE1-EE8 (78.8 – 100%), EE9-Ab9 (94.1%), EE10-EE10* (100%), EE11-Ab54 (89.7%), and EE12-EE15 (83.7 – 89.7%). Figure 2 shows the temporal and spatial distribution of the endemic/epidemic clones.Figure 1


Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital.

Villalón P, Valdezate S, Cabezas T, Ortega M, Garrido N, Vindel A, Medina-Pascual MJ, Saez-Nieto JA - BMC Microbiol. (2015)

Distribution of endemic and epidemicAcinetobacter baumanniiPFGE types. The top chart shows the temporal distribution of the 15 endemic and epidemic PFGE types, and the sequence types (ST). Vertical arrows indicate the nosocomial outbreaks. The bottom table shows the detailed data of the temporal and spatial distribution of the PFGE types. Abreviations: 2 F, second floor; 3 F, third floor; 4 F, fourth floor; 5 F, fifth floor; ICU, intensive care unit; SR, surgery room; ER, emergency room; OC; out-patient clinic consultation room.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4352537&req=5

Fig2: Distribution of endemic and epidemicAcinetobacter baumanniiPFGE types. The top chart shows the temporal distribution of the 15 endemic and epidemic PFGE types, and the sequence types (ST). Vertical arrows indicate the nosocomial outbreaks. The bottom table shows the detailed data of the temporal and spatial distribution of the PFGE types. Abreviations: 2 F, second floor; 3 F, third floor; 4 F, fourth floor; 5 F, fifth floor; ICU, intensive care unit; SR, surgery room; ER, emergency room; OC; out-patient clinic consultation room.
Mentions: Figure 1 shows the PFGE genetic similarity dendogram. The PFGE types were numbered EE1 to EE15. The Hunter-Gaston diversity index (HGDI) [14] calculated for the PFGE technique with respect to the further-examined 231 isolates was 0.880 (Table 1). The genetic similarity range for their 15 PFGE types was 60.4 – 100%. Five clusters (75% cut-off) were observed; their genetic similarity values were EE1-EE8 (78.8 – 100%), EE9-Ab9 (94.1%), EE10-EE10* (100%), EE11-Ab54 (89.7%), and EE12-EE15 (83.7 – 89.7%). Figure 2 shows the temporal and spatial distribution of the endemic/epidemic clones.Figure 1

Bottom Line: MLVA-8Orsay returned 17 allelic profiles.MLST and the VNTR L-markers grouped the isolates into clonal clusters.The wide diversity of MLVA small (S)-markers, however, did not permit clustering.

View Article: PubMed Central - PubMed

Affiliation: Laboratorio de Taxonomía, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda-Pozuelo km2, 28220, Madrid, Majadahonda, Spain. pvillalon@isciii.es.

ABSTRACT

Background: Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period.

Results: PFGE analysis of 405 A. baumannii-calcoaceticus complex isolates detected 15 A. baumannii endemic/epidemic PFGE types (EE1 to EE15) that grouped into five clusters: EE1-EE8, EE9, EE10, EE11 and EE12-EE15. The MLST sequence type (ST) distributions were: international clone II (ST-2) 60%, international clone III (ST-3) 26.7%, ST-15 6.7%, and ST-80 6.7%. MLVA-8Orsay returned 17 allelic profiles. The large (L) VNTR marker profiles were fully concordant with the detected STs, and concordant with 14 up to 15 PFGE types. Imipenem resistance was detected in five PFGE types; the prevalence of the bla OXA-58-like and bla OXA-40-like genes was 60% and 40% respectively.

Conclusions: PFGE proved to be a vital tool for analysis of the temporal and spatial distribution of the clones. MLST and the VNTR L-markers grouped the isolates into clonal clusters. The wide diversity of MLVA small (S)-markers, however, did not permit clustering. The present results demonstrate the persistence of several endemic PFGE types in the hospital, the involvement of some of them in outbreaks, and the inter hospital transmission of extensively drug-resistant ST-15 and ST-80.

Show MeSH
Related in: MedlinePlus