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Immunomudulatory effects of hydroalcoholic extract of Hypericum perforatum.

Abtahi Froushani SM, Esmaili Gouvarchin Galee H, Khamisabadi M, Lotfallahzade B - Avicenna J Phytomed (2015 Jan-Feb)

Bottom Line: Mice in the treatment group orally received hydroalcoholic extract of H. perforatum (110 mg/Kg daily) from the beginning of the study which continued for 2 weeks.Moreover, extract caused a significant reduction in the production of pro-inflammatory IL-17 as well as IFN-γ, parallel to increasing the level of IL-6.The hydroalcoholic extract of H. perforatum may be used as a natural source for treatment of immunopathologic conditions.

View Article: PubMed Central - PubMed

Affiliation:   Assistant Professor of Immunology, Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objective: Hypericum perforatum (St. John's Wort) has long been used in traditional medicine to treat a variety of internal and external ailments. The present study was done to evaluate the immumodulatory potentials of the hydroalcoholic extract of H. perforatum.

Materials and methods: Twenty male BALB/c-mice were randomly allocated in two equal groups and immunized with sheep red blood cells (SRBCs) and complete Freund's adjuvant. Mice in the treatment group orally received hydroalcoholic extract of H. perforatum (110 mg/Kg daily) from the beginning of the study which continued for 2 weeks.

Results: The data indicated a significant increase in the level of anti-SRBC antibody and simultaneously a significant decrease in the level of cellular immunity, an enhancement in foot pad thickness, in treatment group compared to control group. The level of the respiratory burst in phagocytic cells and the level of lymphocyte proliferation in splenocytes were significantly decreased in the treatment group compared to control group. Moreover, extract caused a significant reduction in the production of pro-inflammatory IL-17 as well as IFN-γ, parallel to increasing the level of IL-6.

Conclusions: The hydroalcoholic extract of H. perforatum may be used as a natural source for treatment of immunopathologic conditions.

No MeSH data available.


Cytokines production assay after treatment with hydroalcoholic extract of Hypericum perforatum. Spleen cells isolated from immunized mice with SRBC were cultured with 50 µl of PHA (1 mg/ml) for 72 h. The levels of IFN-γ, IL-17, and IL-10 in culture supernatants were determined after 72 h by ELISA. The results were shown as mean±SD. (* p < 0.001, versus control mice).
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Figure 1: Cytokines production assay after treatment with hydroalcoholic extract of Hypericum perforatum. Spleen cells isolated from immunized mice with SRBC were cultured with 50 µl of PHA (1 mg/ml) for 72 h. The levels of IFN-γ, IL-17, and IL-10 in culture supernatants were determined after 72 h by ELISA. The results were shown as mean±SD. (* p < 0.001, versus control mice).

Mentions: Administration of extract alleviated production of IL-17, IFN-γ, lymphocyte proliferation index, and NBT dye reduction test. A significant decrease in secretion of IL-17 and IFN-γ in cells from extract-treated mice were found compared to cells from vehicle-treated group (Figure 1). The level of IL-10 diminished in the treatment group but this reduction was not significant (Figure 1).


Immunomudulatory effects of hydroalcoholic extract of Hypericum perforatum.

Abtahi Froushani SM, Esmaili Gouvarchin Galee H, Khamisabadi M, Lotfallahzade B - Avicenna J Phytomed (2015 Jan-Feb)

Cytokines production assay after treatment with hydroalcoholic extract of Hypericum perforatum. Spleen cells isolated from immunized mice with SRBC were cultured with 50 µl of PHA (1 mg/ml) for 72 h. The levels of IFN-γ, IL-17, and IL-10 in culture supernatants were determined after 72 h by ELISA. The results were shown as mean±SD. (* p < 0.001, versus control mice).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352534&req=5

Figure 1: Cytokines production assay after treatment with hydroalcoholic extract of Hypericum perforatum. Spleen cells isolated from immunized mice with SRBC were cultured with 50 µl of PHA (1 mg/ml) for 72 h. The levels of IFN-γ, IL-17, and IL-10 in culture supernatants were determined after 72 h by ELISA. The results were shown as mean±SD. (* p < 0.001, versus control mice).
Mentions: Administration of extract alleviated production of IL-17, IFN-γ, lymphocyte proliferation index, and NBT dye reduction test. A significant decrease in secretion of IL-17 and IFN-γ in cells from extract-treated mice were found compared to cells from vehicle-treated group (Figure 1). The level of IL-10 diminished in the treatment group but this reduction was not significant (Figure 1).

Bottom Line: Mice in the treatment group orally received hydroalcoholic extract of H. perforatum (110 mg/Kg daily) from the beginning of the study which continued for 2 weeks.Moreover, extract caused a significant reduction in the production of pro-inflammatory IL-17 as well as IFN-γ, parallel to increasing the level of IL-6.The hydroalcoholic extract of H. perforatum may be used as a natural source for treatment of immunopathologic conditions.

View Article: PubMed Central - PubMed

Affiliation:   Assistant Professor of Immunology, Department of Microbiology, Veterinary Faculty, Urmia University, Urmia, Iran.

ABSTRACT

Objective: Hypericum perforatum (St. John's Wort) has long been used in traditional medicine to treat a variety of internal and external ailments. The present study was done to evaluate the immumodulatory potentials of the hydroalcoholic extract of H. perforatum.

Materials and methods: Twenty male BALB/c-mice were randomly allocated in two equal groups and immunized with sheep red blood cells (SRBCs) and complete Freund's adjuvant. Mice in the treatment group orally received hydroalcoholic extract of H. perforatum (110 mg/Kg daily) from the beginning of the study which continued for 2 weeks.

Results: The data indicated a significant increase in the level of anti-SRBC antibody and simultaneously a significant decrease in the level of cellular immunity, an enhancement in foot pad thickness, in treatment group compared to control group. The level of the respiratory burst in phagocytic cells and the level of lymphocyte proliferation in splenocytes were significantly decreased in the treatment group compared to control group. Moreover, extract caused a significant reduction in the production of pro-inflammatory IL-17 as well as IFN-γ, parallel to increasing the level of IL-6.

Conclusions: The hydroalcoholic extract of H. perforatum may be used as a natural source for treatment of immunopathologic conditions.

No MeSH data available.