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Pomegranate seed hydroalcoholic extract improves memory deficits in ovariectomized rats with permanent cerebral hypoperfusion /ischemia.

Sarkaki A, Farbood Y, Hashemi S, Rafiei Rad M - Avicenna J Phytomed (2015 Jan-Feb)

Bottom Line: PGSE improved it significantly in dose dependently manner (P<0.001 vs.OI+Veh).PGSE treatment significantly improved memories in OI groups (P<0.05, P<0.01 and P<0.001) compared with OI+Veh.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Physiology, Medicine Faculty, Ahvaz Jundishpur University of Medical Sciences, Ahvaz-Iran ; Physiology Research Center, Ahvaz Jundishpur University of Medical Sciences, Ahvaz-Iran.

ABSTRACT

Objectives: Estrogen deficit following menopause results in cognitive behaviors impairment. This study aimed to evaluate the effects of pomegranate seed extract (PGSE) on avoidance memories after permanent bilateral common carotid arteries occlusion (2CCAO) in ovariectomized (OVX) rats.

Materials and methods: Adult female Wistar rats were divided randomly into eight groups with 8 rats in each group: 1) Sham-operated for ovaries and 2CCAO (ShO); 2) OVX and sham operated for ischemia (OShI); 3-7) OVX with 2CCAO (OI) received PGSE (100, 200, 400 and 800 mg/2ml/kg or normal saline, orally) for 14 days (OI+E100, 200, 400, 800 or OI+Veh); 8) OShI received most effective dose of PGSE (200 and 400 mg/kg for passive and active avoidance memories respectively). Active and passive avoidance tasks were measured in Y-maze and two-way shuttle box respectively. Data were analyzed with one-way and RM-ANOVA followed by HSD post-hoc test.

Results: Sensorimotor impaired in OShI+Veh and OI+Veh (P<0.001 vs. ShO). PGSE improved it significantly in dose dependently manner (P<0.001 vs. OI+Veh). Both types of memories were significantly impaired in OVX rats before and after 2CCAO (P<0.001). PGSE treatment significantly improved memories in OI groups (P<0.05, P<0.01 and P<0.001) compared with OI+Veh. No toxicity was observed with PGSE consumption (800 mg/kg, 2 weeks, orally).

Conclusion: PGSE exhibits therapeutic potential for avoidance memories, which is most likely related at least in part to its phytoestrogenic and also antioxidative actions.

No MeSH data available.


Related in: MedlinePlus

The percents of criterion condition responses (%CCRs) during four sessions training in Y-maze in different groups after two weeks treatment with PGSE in ovariectomized without and with 2CCAO groups. ***P<0.001 for significant difference with ShO group and ###P<0.001 for significant difference versus OShI+Veh and OI+Veh groups. Repeated measured (RM) ANOVA analysis that followed by HSD post hoc test. ShO: sham operated for ovariectomy, OI: ovariectomized rats with 2CCAO, OI+E100-800: ovariectomized rats with 2CCAO received different doses of PGSE, OI+Veh: ovariectomized rats with 2CCAO received normal saline, OShI+E200 and OShI+Veh: ovariectomized rats with sham operated for 2CCAO received 200 mg/kg PGSE and normal saline respectively.
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Figure 4: The percents of criterion condition responses (%CCRs) during four sessions training in Y-maze in different groups after two weeks treatment with PGSE in ovariectomized without and with 2CCAO groups. ***P<0.001 for significant difference with ShO group and ###P<0.001 for significant difference versus OShI+Veh and OI+Veh groups. Repeated measured (RM) ANOVA analysis that followed by HSD post hoc test. ShO: sham operated for ovariectomy, OI: ovariectomized rats with 2CCAO, OI+E100-800: ovariectomized rats with 2CCAO received different doses of PGSE, OI+Veh: ovariectomized rats with 2CCAO received normal saline, OShI+E200 and OShI+Veh: ovariectomized rats with sham operated for 2CCAO received 200 mg/kg PGSE and normal saline respectively.

Mentions: Figure 4 shows that the percents of criterion condition responses (%CCRs) was lower significantly after ovariectomy without 2CCAO group (OShI+Veh) during 4 days training in Y-maze (P<0.001). In this test, 400 mg/kg of PGSE was the best effective dose in active avoidance memory and treatment OShI group with this dose (OShI+E400) reversed it to normal range (P<0.001, fig. 4a).


Pomegranate seed hydroalcoholic extract improves memory deficits in ovariectomized rats with permanent cerebral hypoperfusion /ischemia.

Sarkaki A, Farbood Y, Hashemi S, Rafiei Rad M - Avicenna J Phytomed (2015 Jan-Feb)

The percents of criterion condition responses (%CCRs) during four sessions training in Y-maze in different groups after two weeks treatment with PGSE in ovariectomized without and with 2CCAO groups. ***P<0.001 for significant difference with ShO group and ###P<0.001 for significant difference versus OShI+Veh and OI+Veh groups. Repeated measured (RM) ANOVA analysis that followed by HSD post hoc test. ShO: sham operated for ovariectomy, OI: ovariectomized rats with 2CCAO, OI+E100-800: ovariectomized rats with 2CCAO received different doses of PGSE, OI+Veh: ovariectomized rats with 2CCAO received normal saline, OShI+E200 and OShI+Veh: ovariectomized rats with sham operated for 2CCAO received 200 mg/kg PGSE and normal saline respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352532&req=5

Figure 4: The percents of criterion condition responses (%CCRs) during four sessions training in Y-maze in different groups after two weeks treatment with PGSE in ovariectomized without and with 2CCAO groups. ***P<0.001 for significant difference with ShO group and ###P<0.001 for significant difference versus OShI+Veh and OI+Veh groups. Repeated measured (RM) ANOVA analysis that followed by HSD post hoc test. ShO: sham operated for ovariectomy, OI: ovariectomized rats with 2CCAO, OI+E100-800: ovariectomized rats with 2CCAO received different doses of PGSE, OI+Veh: ovariectomized rats with 2CCAO received normal saline, OShI+E200 and OShI+Veh: ovariectomized rats with sham operated for 2CCAO received 200 mg/kg PGSE and normal saline respectively.
Mentions: Figure 4 shows that the percents of criterion condition responses (%CCRs) was lower significantly after ovariectomy without 2CCAO group (OShI+Veh) during 4 days training in Y-maze (P<0.001). In this test, 400 mg/kg of PGSE was the best effective dose in active avoidance memory and treatment OShI group with this dose (OShI+E400) reversed it to normal range (P<0.001, fig. 4a).

Bottom Line: PGSE improved it significantly in dose dependently manner (P<0.001 vs.OI+Veh).PGSE treatment significantly improved memories in OI groups (P<0.05, P<0.01 and P<0.001) compared with OI+Veh.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Physiology, Medicine Faculty, Ahvaz Jundishpur University of Medical Sciences, Ahvaz-Iran ; Physiology Research Center, Ahvaz Jundishpur University of Medical Sciences, Ahvaz-Iran.

ABSTRACT

Objectives: Estrogen deficit following menopause results in cognitive behaviors impairment. This study aimed to evaluate the effects of pomegranate seed extract (PGSE) on avoidance memories after permanent bilateral common carotid arteries occlusion (2CCAO) in ovariectomized (OVX) rats.

Materials and methods: Adult female Wistar rats were divided randomly into eight groups with 8 rats in each group: 1) Sham-operated for ovaries and 2CCAO (ShO); 2) OVX and sham operated for ischemia (OShI); 3-7) OVX with 2CCAO (OI) received PGSE (100, 200, 400 and 800 mg/2ml/kg or normal saline, orally) for 14 days (OI+E100, 200, 400, 800 or OI+Veh); 8) OShI received most effective dose of PGSE (200 and 400 mg/kg for passive and active avoidance memories respectively). Active and passive avoidance tasks were measured in Y-maze and two-way shuttle box respectively. Data were analyzed with one-way and RM-ANOVA followed by HSD post-hoc test.

Results: Sensorimotor impaired in OShI+Veh and OI+Veh (P<0.001 vs. ShO). PGSE improved it significantly in dose dependently manner (P<0.001 vs. OI+Veh). Both types of memories were significantly impaired in OVX rats before and after 2CCAO (P<0.001). PGSE treatment significantly improved memories in OI groups (P<0.05, P<0.01 and P<0.001) compared with OI+Veh. No toxicity was observed with PGSE consumption (800 mg/kg, 2 weeks, orally).

Conclusion: PGSE exhibits therapeutic potential for avoidance memories, which is most likely related at least in part to its phytoestrogenic and also antioxidative actions.

No MeSH data available.


Related in: MedlinePlus