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Antioxidant activity and total phenolic content of Boerhavia elegans (choisy) grown in Baluchestan, Iran.

Sadeghi Z, Valizadeh J, Azyzian Shermeh O, Akaberi M - Avicenna J Phytomed (2015 Jan-Feb)

Bottom Line: Antioxidant results were expressed as IC50.The results of the experiments showed that B. elegans extract had significant antioxidant effects.This high antioxidant activity may be linked to phenolic contents of the plant but complementary investigations are suggested in order to determine active elements.

View Article: PubMed Central - PubMed

Affiliation: Department of Production and Utilization of Medicinal Plants, Faculty of Agricultural and Natural Resources, Higher Educational Complex of Saravan, Saravan, I. R. Iran.

ABSTRACT

Objective: Boerhaavia elegans L. (Nyctaginaceae) is a medicinal plant used for the treatment of kidney disorders, urinary tract disorders and blood purification in Baluch tribe. The aim of present study is to evaluate the antioxidant property of B. elegans species for the first time.

Materials and methods: Different parts (leaf, stem and fruit) of the plant were extracted by using various solvents (water, methanol, chloroform and ethyl acetate) and evaluated for their antioxidant activity using DPPH (2, 2-diphenyl-1 picryl hydrazyl) and FRAP (ferric reducing antioxidant power) methods. In addition, total phenolic content was determined by Folin-Ciocalteu reagent.

Results: Antioxidant results were expressed as IC50. The antioxidant power in DPPH and FRAP assay were evaluated as shown in decreasing order: Methanolic extract > Aqueous extract > Ethyl acetate extract > Chloroform extract, for all parts of the plant. In both methods of antioxidant assay and Folin-Ciocalteu method, methanolic extract of leaf exhibited the highest activity and the most phenolic content IC50= 6.85 ppm and 16.41 mg GA/g d w respectively. Total phenolic content had a positive relationship with antioxidant capacity in extracts and there was a high correlation (r=1.00, p<0.01) between antioxidant activities as determined by both antioxidant assays for various parts.

Conclusion: The results of the experiments showed that B. elegans extract had significant antioxidant effects. This high antioxidant activity may be linked to phenolic contents of the plant but complementary investigations are suggested in order to determine active elements.

No MeSH data available.


Related in: MedlinePlus

B. elegans; flower, shrub and leaf
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Figure 1: B. elegans; flower, shrub and leaf

Mentions: Boerhavia elegans is a permanent plant with an erect glabrous shrub up to 1 m high and a burly root stock. Its fleshy stems become woody towards the base, which is dark green, often heated with red, glabrescent to pubescent, forking mainly from the base with the nodes ventricose. The leaves broadly are ovate-lanceolate or linear-lanceolate, green-gray above, pubescent-cansecent beneath, petiole 1cm long or absent; caulines sessile or shortly petiolate (Figure 1) (Chen et al., 2007 ▶; Ghahraman, 1996 ▶; Ramazani et al., 2010 ▶; Mahesh et al., 2012 ▶).


Antioxidant activity and total phenolic content of Boerhavia elegans (choisy) grown in Baluchestan, Iran.

Sadeghi Z, Valizadeh J, Azyzian Shermeh O, Akaberi M - Avicenna J Phytomed (2015 Jan-Feb)

B. elegans; flower, shrub and leaf
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352527&req=5

Figure 1: B. elegans; flower, shrub and leaf
Mentions: Boerhavia elegans is a permanent plant with an erect glabrous shrub up to 1 m high and a burly root stock. Its fleshy stems become woody towards the base, which is dark green, often heated with red, glabrescent to pubescent, forking mainly from the base with the nodes ventricose. The leaves broadly are ovate-lanceolate or linear-lanceolate, green-gray above, pubescent-cansecent beneath, petiole 1cm long or absent; caulines sessile or shortly petiolate (Figure 1) (Chen et al., 2007 ▶; Ghahraman, 1996 ▶; Ramazani et al., 2010 ▶; Mahesh et al., 2012 ▶).

Bottom Line: Antioxidant results were expressed as IC50.The results of the experiments showed that B. elegans extract had significant antioxidant effects.This high antioxidant activity may be linked to phenolic contents of the plant but complementary investigations are suggested in order to determine active elements.

View Article: PubMed Central - PubMed

Affiliation: Department of Production and Utilization of Medicinal Plants, Faculty of Agricultural and Natural Resources, Higher Educational Complex of Saravan, Saravan, I. R. Iran.

ABSTRACT

Objective: Boerhaavia elegans L. (Nyctaginaceae) is a medicinal plant used for the treatment of kidney disorders, urinary tract disorders and blood purification in Baluch tribe. The aim of present study is to evaluate the antioxidant property of B. elegans species for the first time.

Materials and methods: Different parts (leaf, stem and fruit) of the plant were extracted by using various solvents (water, methanol, chloroform and ethyl acetate) and evaluated for their antioxidant activity using DPPH (2, 2-diphenyl-1 picryl hydrazyl) and FRAP (ferric reducing antioxidant power) methods. In addition, total phenolic content was determined by Folin-Ciocalteu reagent.

Results: Antioxidant results were expressed as IC50. The antioxidant power in DPPH and FRAP assay were evaluated as shown in decreasing order: Methanolic extract > Aqueous extract > Ethyl acetate extract > Chloroform extract, for all parts of the plant. In both methods of antioxidant assay and Folin-Ciocalteu method, methanolic extract of leaf exhibited the highest activity and the most phenolic content IC50= 6.85 ppm and 16.41 mg GA/g d w respectively. Total phenolic content had a positive relationship with antioxidant capacity in extracts and there was a high correlation (r=1.00, p<0.01) between antioxidant activities as determined by both antioxidant assays for various parts.

Conclusion: The results of the experiments showed that B. elegans extract had significant antioxidant effects. This high antioxidant activity may be linked to phenolic contents of the plant but complementary investigations are suggested in order to determine active elements.

No MeSH data available.


Related in: MedlinePlus