Limits...
Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.

Wang G, Yao S, Zhang XX, Song H - Int J Anal Chem (2015)

Bottom Line: The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids.Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant.The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

View Article: PubMed Central - PubMed

Affiliation: Zunyi Medical College, Zunyi, Guizhou 563003, China ; Sichuan University, Chengdu, Sichuan 610000, China.

ABSTRACT
2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

No MeSH data available.


Related in: MedlinePlus

Proposed fragmentation pathways of hinokiflavone.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352518&req=5

fig5: Proposed fragmentation pathways of hinokiflavone.

Mentions: Proposed fragmentation pathways of hinokiflavone were shown in Figure 5. Compound 6 exhibited the quasimolecular ion [M+H]+ at m/z 539.0963. The most main diagnostic ions are a sequence of ions at m/z 286, 270, 254, and 242, which root in the rupture of the C-O connection on flavonoid parts I and II (see Figure 5). Furthermore, the predominant ion at m/z 257 is found by the quasimolecular ion losing flavonoid part I and CO, continuously. So compound 6 was determined as hinokiflavone on the basis of previous deduction and literature [19]. Through the above analysis, the major fragmentation of the hinokiflavone-type biflavonoids shows great difference with the first two types of biflavonoids, because two flavonoid parts of the hinokiflavone type are not a C-C bond but a C-O bond.


Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.

Wang G, Yao S, Zhang XX, Song H - Int J Anal Chem (2015)

Proposed fragmentation pathways of hinokiflavone.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352518&req=5

fig5: Proposed fragmentation pathways of hinokiflavone.
Mentions: Proposed fragmentation pathways of hinokiflavone were shown in Figure 5. Compound 6 exhibited the quasimolecular ion [M+H]+ at m/z 539.0963. The most main diagnostic ions are a sequence of ions at m/z 286, 270, 254, and 242, which root in the rupture of the C-O connection on flavonoid parts I and II (see Figure 5). Furthermore, the predominant ion at m/z 257 is found by the quasimolecular ion losing flavonoid part I and CO, continuously. So compound 6 was determined as hinokiflavone on the basis of previous deduction and literature [19]. Through the above analysis, the major fragmentation of the hinokiflavone-type biflavonoids shows great difference with the first two types of biflavonoids, because two flavonoid parts of the hinokiflavone type are not a C-C bond but a C-O bond.

Bottom Line: The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids.Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant.The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

View Article: PubMed Central - PubMed

Affiliation: Zunyi Medical College, Zunyi, Guizhou 563003, China ; Sichuan University, Chengdu, Sichuan 610000, China.

ABSTRACT
2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

No MeSH data available.


Related in: MedlinePlus