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Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.

Wang G, Yao S, Zhang XX, Song H - Int J Anal Chem (2015)

Bottom Line: The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids.Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant.The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

View Article: PubMed Central - PubMed

Affiliation: Zunyi Medical College, Zunyi, Guizhou 563003, China ; Sichuan University, Chengdu, Sichuan 610000, China.

ABSTRACT
2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

No MeSH data available.


Related in: MedlinePlus

Chromatograms of the ethyl acetate fraction of Selaginella doederleinii before and after reaction with DPPH radicals.
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fig2: Chromatograms of the ethyl acetate fraction of Selaginella doederleinii before and after reaction with DPPH radicals.

Mentions: According to the UPLC conditions in Section 2.4, the quick analysis results of the potential antioxidants from Selaginella doederleinii are shown in Figure 2. An antioxidant and a DPPH radical could mingle and produce the oxidation reaction. After that, the oxidation would change the molecular structure of the antioxidant [16]. It was concluded that the peak areas (PAs) of the antioxidant would obviously decrease in the UPLC chromatogram after reaction with DPPH. But the PAs of the compounds without antioxidant effect witnessed almost no change. Therefore, the untreated and DPPH-treated ethyl acetate fraction from Selaginella doederleinii were analyzed by the optimized UPLC separation conditions as stated. Through the comparison of the UPLC chromatograms of untreated and DPPH-treated samples, the PAs of nine main peaks were all decreased significantly. And nine peaks were detected and isolated from the sample. Their retention time was 5.25, 6.19, 7.28, 8.32, 8.81, 9.27, 10.92, 11.47, and 14.92 min, respectively (see Figure 2). The results indicated that these nine peaks were all antioxidants existing in the ethyl acetate fraction.


Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.

Wang G, Yao S, Zhang XX, Song H - Int J Anal Chem (2015)

Chromatograms of the ethyl acetate fraction of Selaginella doederleinii before and after reaction with DPPH radicals.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352518&req=5

fig2: Chromatograms of the ethyl acetate fraction of Selaginella doederleinii before and after reaction with DPPH radicals.
Mentions: According to the UPLC conditions in Section 2.4, the quick analysis results of the potential antioxidants from Selaginella doederleinii are shown in Figure 2. An antioxidant and a DPPH radical could mingle and produce the oxidation reaction. After that, the oxidation would change the molecular structure of the antioxidant [16]. It was concluded that the peak areas (PAs) of the antioxidant would obviously decrease in the UPLC chromatogram after reaction with DPPH. But the PAs of the compounds without antioxidant effect witnessed almost no change. Therefore, the untreated and DPPH-treated ethyl acetate fraction from Selaginella doederleinii were analyzed by the optimized UPLC separation conditions as stated. Through the comparison of the UPLC chromatograms of untreated and DPPH-treated samples, the PAs of nine main peaks were all decreased significantly. And nine peaks were detected and isolated from the sample. Their retention time was 5.25, 6.19, 7.28, 8.32, 8.81, 9.27, 10.92, 11.47, and 14.92 min, respectively (see Figure 2). The results indicated that these nine peaks were all antioxidants existing in the ethyl acetate fraction.

Bottom Line: The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids.Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant.The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

View Article: PubMed Central - PubMed

Affiliation: Zunyi Medical College, Zunyi, Guizhou 563003, China ; Sichuan University, Chengdu, Sichuan 610000, China.

ABSTRACT
2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

No MeSH data available.


Related in: MedlinePlus