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Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.

Wang G, Yao S, Zhang XX, Song H - Int J Anal Chem (2015)

Bottom Line: The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids.Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant.The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

View Article: PubMed Central - PubMed

Affiliation: Zunyi Medical College, Zunyi, Guizhou 563003, China ; Sichuan University, Chengdu, Sichuan 610000, China.

ABSTRACT
2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of bioflavonoids in Selaginella doederleinii.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352518&req=5

fig1: Chemical structures of bioflavonoids in Selaginella doederleinii.

Mentions: Some researches [7] have proposed that the potential antioxidants in Selaginella doederleinii are biflavonoids, including amentoflavone, robustaflavone, heveaflavone, and robustaflavone 4′,  4′′′-O-dimethyl ether (see Figure 1). Most biflavonoids, just like apigenin and its methoxyl/hydroxyl substituents, have prominent antioxidant characteristics and show a wide range of biological activity, such as cancer chemopreventive properties, anti-inflammatory, antimicroorganism, and antioxidative effects [8–13].


Rapid Screening and Structural Characterization of Antioxidants from the Extract of Selaginella doederleinii Hieron with DPPH-UPLC-Q-TOF/MS Method.

Wang G, Yao S, Zhang XX, Song H - Int J Anal Chem (2015)

Chemical structures of bioflavonoids in Selaginella doederleinii.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352518&req=5

fig1: Chemical structures of bioflavonoids in Selaginella doederleinii.
Mentions: Some researches [7] have proposed that the potential antioxidants in Selaginella doederleinii are biflavonoids, including amentoflavone, robustaflavone, heveaflavone, and robustaflavone 4′,  4′′′-O-dimethyl ether (see Figure 1). Most biflavonoids, just like apigenin and its methoxyl/hydroxyl substituents, have prominent antioxidant characteristics and show a wide range of biological activity, such as cancer chemopreventive properties, anti-inflammatory, antimicroorganism, and antioxidative effects [8–13].

Bottom Line: The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids.Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant.The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

View Article: PubMed Central - PubMed

Affiliation: Zunyi Medical College, Zunyi, Guizhou 563003, China ; Sichuan University, Chengdu, Sichuan 610000, China.

ABSTRACT
2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types, that is, amentoflavone-type, robustaflavone-type, and hinokiflavone-type biflavonoids. Among the compounds bilobetin (3) and putraflavone (8) were found from Selaginella doederleinii for the first time and others including amentoflavone (1), robustaflavone (2), 4'-methoxy robustaflavone (4), podocarpusflavone A (5), hinokiflavone (6), ginkgetin (7), and heveaflavone (9) were identified previously in the plant. Moreover, nine biflavones possessed a good antioxidant activity via their DPPH free radical scavenging. It demonstrates that DPPH-UPLC-Q-TOF/MS exhibits strong capacity in separation and identification for small molecule. The method is suitable for rapid screening of antioxidants without the need for complicated systems and additional instruments.

No MeSH data available.


Related in: MedlinePlus