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Lespedeza davurica (Lax.) Schindl. extract protects against cytokine-induced β-cell damage and streptozotocin-induced diabetes.

Sharma BR, Rhyu DY - Biomed Res Int (2015)

Bottom Line: RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage.The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation.In OGTT, glucose clearance levels improved by oral treatment of LD extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, College of Natural Science, Mokpo National University, 1666 Youngsan-ro, Muan-gun, Jeonnam 534-729, Republic of Korea.

ABSTRACT
Lespedeza has been used for the management of diabetes in folklore medicine. The purpose of this study is to investigate the protective effects of the methanol extract of Lespedeza davurica (LD) on cytokine-induced β-cell damage and streptozotocin- (STZ-) induced diabetes. RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage. The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation. Antidiabetic effects of LD extract were observed by oral glucose tolerance test (OGTT) in normal rats and by checking the biochemical, physiological, and histopathological parameters in STZ-induced diabetic rats. In OGTT, glucose clearance levels improved by oral treatment of LD extract. The water intake, urine volume, blood glucose, and serum TG, TC, TBARS, and DPP-IV levels were significantly decreased, and liver glycogen content was significantly increased by treatment of LD extract (250 mg/kg BW) in STZ-induced diabetic rats. Also, insulin immunoreactivity of the pancreases was increased in LD extract administrated rats compared with diabetic control rats. These results indicate that LD extract may protect pancreatic β-cell damage and regulate the blood glucose in STZ-induced diabetic rats.

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Related in: MedlinePlus

Immunohistochemical (IHC) and H&E staining of pancreatic islets in STZ-induced diabetic rats. Pancreases were obtained from control ((a), (e)), diabetic control ((b), (f)), LD-treated diabetic group ((c), (g)), and TLB-treated diabetic group ((d), (h)). Islets and adjoining exocrine regions were stained with H&E ((a)–(d)). Islets were labeled with an anti-insulin antibody and peroxidase-labeled anti-rabbit IgG ((e)–(h)). Scale bar is 100 μm. LD (L. davurica) and TLB (tolbutamide) were administered at a dose of 250 mg/kg BW for 4 weeks.
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fig6: Immunohistochemical (IHC) and H&E staining of pancreatic islets in STZ-induced diabetic rats. Pancreases were obtained from control ((a), (e)), diabetic control ((b), (f)), LD-treated diabetic group ((c), (g)), and TLB-treated diabetic group ((d), (h)). Islets and adjoining exocrine regions were stained with H&E ((a)–(d)). Islets were labeled with an anti-insulin antibody and peroxidase-labeled anti-rabbit IgG ((e)–(h)). Scale bar is 100 μm. LD (L. davurica) and TLB (tolbutamide) were administered at a dose of 250 mg/kg BW for 4 weeks.

Mentions: The effects of LD extract on pancreatic β-cell damage in STZ-induced diabetic rats were examined histologically. Pancreatic tissues were subjected to H&E staining and immunohistochemistry. STZ-treated rats showed degenerative and necrotic changes and islet shrinkage as well as weak insulin reactivity. However, LD extract-treated tissues showed round and clearly defined islets that were strongly positive for insulin (Figure 6).


Lespedeza davurica (Lax.) Schindl. extract protects against cytokine-induced β-cell damage and streptozotocin-induced diabetes.

Sharma BR, Rhyu DY - Biomed Res Int (2015)

Immunohistochemical (IHC) and H&E staining of pancreatic islets in STZ-induced diabetic rats. Pancreases were obtained from control ((a), (e)), diabetic control ((b), (f)), LD-treated diabetic group ((c), (g)), and TLB-treated diabetic group ((d), (h)). Islets and adjoining exocrine regions were stained with H&E ((a)–(d)). Islets were labeled with an anti-insulin antibody and peroxidase-labeled anti-rabbit IgG ((e)–(h)). Scale bar is 100 μm. LD (L. davurica) and TLB (tolbutamide) were administered at a dose of 250 mg/kg BW for 4 weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352516&req=5

fig6: Immunohistochemical (IHC) and H&E staining of pancreatic islets in STZ-induced diabetic rats. Pancreases were obtained from control ((a), (e)), diabetic control ((b), (f)), LD-treated diabetic group ((c), (g)), and TLB-treated diabetic group ((d), (h)). Islets and adjoining exocrine regions were stained with H&E ((a)–(d)). Islets were labeled with an anti-insulin antibody and peroxidase-labeled anti-rabbit IgG ((e)–(h)). Scale bar is 100 μm. LD (L. davurica) and TLB (tolbutamide) were administered at a dose of 250 mg/kg BW for 4 weeks.
Mentions: The effects of LD extract on pancreatic β-cell damage in STZ-induced diabetic rats were examined histologically. Pancreatic tissues were subjected to H&E staining and immunohistochemistry. STZ-treated rats showed degenerative and necrotic changes and islet shrinkage as well as weak insulin reactivity. However, LD extract-treated tissues showed round and clearly defined islets that were strongly positive for insulin (Figure 6).

Bottom Line: RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage.The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation.In OGTT, glucose clearance levels improved by oral treatment of LD extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, College of Natural Science, Mokpo National University, 1666 Youngsan-ro, Muan-gun, Jeonnam 534-729, Republic of Korea.

ABSTRACT
Lespedeza has been used for the management of diabetes in folklore medicine. The purpose of this study is to investigate the protective effects of the methanol extract of Lespedeza davurica (LD) on cytokine-induced β-cell damage and streptozotocin- (STZ-) induced diabetes. RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage. The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation. Antidiabetic effects of LD extract were observed by oral glucose tolerance test (OGTT) in normal rats and by checking the biochemical, physiological, and histopathological parameters in STZ-induced diabetic rats. In OGTT, glucose clearance levels improved by oral treatment of LD extract. The water intake, urine volume, blood glucose, and serum TG, TC, TBARS, and DPP-IV levels were significantly decreased, and liver glycogen content was significantly increased by treatment of LD extract (250 mg/kg BW) in STZ-induced diabetic rats. Also, insulin immunoreactivity of the pancreases was increased in LD extract administrated rats compared with diabetic control rats. These results indicate that LD extract may protect pancreatic β-cell damage and regulate the blood glucose in STZ-induced diabetic rats.

Show MeSH
Related in: MedlinePlus