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Lespedeza davurica (Lax.) Schindl. extract protects against cytokine-induced β-cell damage and streptozotocin-induced diabetes.

Sharma BR, Rhyu DY - Biomed Res Int (2015)

Bottom Line: RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage.The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation.In OGTT, glucose clearance levels improved by oral treatment of LD extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, College of Natural Science, Mokpo National University, 1666 Youngsan-ro, Muan-gun, Jeonnam 534-729, Republic of Korea.

ABSTRACT
Lespedeza has been used for the management of diabetes in folklore medicine. The purpose of this study is to investigate the protective effects of the methanol extract of Lespedeza davurica (LD) on cytokine-induced β-cell damage and streptozotocin- (STZ-) induced diabetes. RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage. The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation. Antidiabetic effects of LD extract were observed by oral glucose tolerance test (OGTT) in normal rats and by checking the biochemical, physiological, and histopathological parameters in STZ-induced diabetic rats. In OGTT, glucose clearance levels improved by oral treatment of LD extract. The water intake, urine volume, blood glucose, and serum TG, TC, TBARS, and DPP-IV levels were significantly decreased, and liver glycogen content was significantly increased by treatment of LD extract (250 mg/kg BW) in STZ-induced diabetic rats. Also, insulin immunoreactivity of the pancreases was increased in LD extract administrated rats compared with diabetic control rats. These results indicate that LD extract may protect pancreatic β-cell damage and regulate the blood glucose in STZ-induced diabetic rats.

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Related in: MedlinePlus

Effects of LD extract on cytokine-induced cell death, NO production, and iNOS protein and mRNA expression in RINm5F cells. RINm5F cells (2 × 105) were pretreated with the indicated concentrations of LD extract for 3 h, followed by stimulation with IL-1β (2 ng/mL) and IFN-γ (100 U/mL) for 48 h. Cell viability (a), NO production (b), and iNOS protein and mRNA expression (c) were determined by the MTT assay, Griess reagent, western blotting, and RT-PCR analyses, respectively. Each value represents the mean ± SE of three independent experiments. Bars with different letters are significantly different at P < 0.05 (Duncan's multiple comparison tests).
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fig1: Effects of LD extract on cytokine-induced cell death, NO production, and iNOS protein and mRNA expression in RINm5F cells. RINm5F cells (2 × 105) were pretreated with the indicated concentrations of LD extract for 3 h, followed by stimulation with IL-1β (2 ng/mL) and IFN-γ (100 U/mL) for 48 h. Cell viability (a), NO production (b), and iNOS protein and mRNA expression (c) were determined by the MTT assay, Griess reagent, western blotting, and RT-PCR analyses, respectively. Each value represents the mean ± SE of three independent experiments. Bars with different letters are significantly different at P < 0.05 (Duncan's multiple comparison tests).

Mentions: RINm5F cells were treated with various concentrations of LD extract to assess its cytotoxicity. Then, nontoxic doses of LD (50 and 100 μg/mL) were used for further experiments. As shown in Figure 1(a), the combination of IL-1β and IFN-γ decreased cell viability to 53%. However, the addition of LD extract (50 and 100 μg/mL) increased cell viability to 69 and 81%, respectively.


Lespedeza davurica (Lax.) Schindl. extract protects against cytokine-induced β-cell damage and streptozotocin-induced diabetes.

Sharma BR, Rhyu DY - Biomed Res Int (2015)

Effects of LD extract on cytokine-induced cell death, NO production, and iNOS protein and mRNA expression in RINm5F cells. RINm5F cells (2 × 105) were pretreated with the indicated concentrations of LD extract for 3 h, followed by stimulation with IL-1β (2 ng/mL) and IFN-γ (100 U/mL) for 48 h. Cell viability (a), NO production (b), and iNOS protein and mRNA expression (c) were determined by the MTT assay, Griess reagent, western blotting, and RT-PCR analyses, respectively. Each value represents the mean ± SE of three independent experiments. Bars with different letters are significantly different at P < 0.05 (Duncan's multiple comparison tests).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352516&req=5

fig1: Effects of LD extract on cytokine-induced cell death, NO production, and iNOS protein and mRNA expression in RINm5F cells. RINm5F cells (2 × 105) were pretreated with the indicated concentrations of LD extract for 3 h, followed by stimulation with IL-1β (2 ng/mL) and IFN-γ (100 U/mL) for 48 h. Cell viability (a), NO production (b), and iNOS protein and mRNA expression (c) were determined by the MTT assay, Griess reagent, western blotting, and RT-PCR analyses, respectively. Each value represents the mean ± SE of three independent experiments. Bars with different letters are significantly different at P < 0.05 (Duncan's multiple comparison tests).
Mentions: RINm5F cells were treated with various concentrations of LD extract to assess its cytotoxicity. Then, nontoxic doses of LD (50 and 100 μg/mL) were used for further experiments. As shown in Figure 1(a), the combination of IL-1β and IFN-γ decreased cell viability to 53%. However, the addition of LD extract (50 and 100 μg/mL) increased cell viability to 69 and 81%, respectively.

Bottom Line: RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage.The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation.In OGTT, glucose clearance levels improved by oral treatment of LD extract.

View Article: PubMed Central - PubMed

Affiliation: Department of Oriental Medicine Resources and Institute of Korean Medicine Industry, College of Natural Science, Mokpo National University, 1666 Youngsan-ro, Muan-gun, Jeonnam 534-729, Republic of Korea.

ABSTRACT
Lespedeza has been used for the management of diabetes in folklore medicine. The purpose of this study is to investigate the protective effects of the methanol extract of Lespedeza davurica (LD) on cytokine-induced β-cell damage and streptozotocin- (STZ-) induced diabetes. RINm5F cells were treated with interleukin- (IL-) 1β and interferon- (IFN-) γ to induce pancreatic β-cell damage. The exposure of LD extract significantly decreased cell death, nitric oxide (NO) production, nitric oxide synthase (iNOS) expression, and nucleus factor-kappa B (NF-κB) p65 activation. Antidiabetic effects of LD extract were observed by oral glucose tolerance test (OGTT) in normal rats and by checking the biochemical, physiological, and histopathological parameters in STZ-induced diabetic rats. In OGTT, glucose clearance levels improved by oral treatment of LD extract. The water intake, urine volume, blood glucose, and serum TG, TC, TBARS, and DPP-IV levels were significantly decreased, and liver glycogen content was significantly increased by treatment of LD extract (250 mg/kg BW) in STZ-induced diabetic rats. Also, insulin immunoreactivity of the pancreases was increased in LD extract administrated rats compared with diabetic control rats. These results indicate that LD extract may protect pancreatic β-cell damage and regulate the blood glucose in STZ-induced diabetic rats.

Show MeSH
Related in: MedlinePlus