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Instant transport media for biopsied soft tissue specimens: a comparative study.

Patil S, Rao RS, Agarwal A, Raj AT - Scientifica (Cairo) (2015)

Bottom Line: Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated.Tissues transported in milk showed poor preservation.Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology & Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Bangalore 560054, India.

ABSTRACT
Background. Formalin, a traditionally preferred fixative in the field of pathology, has restricted usage in private settings. Since its toxicity violates the Occupational Safety and Health Administration regulations, an eco-friendly alternative would be the need of the hour. Hence an instant media which is economical and nontoxic and enables easy transport of biopsied soft tissue specimens in its original state is of vital importance. Materials and Methods. Commercially available fresh goat buccal mucosa specimens were sliced into smaller bits of equal dimensions and placed in six different containers containing 20% honey, 30% jaggery, milk, and ice for 1 hr, 6 hours, 12 hours, and 24 hours each with formalin as a positive control. After the set time interval, the specimens were fixed in 10% buffered formalin for 24 hours followed by routine processing and staining. The histologic interpretations were a blinded procedure and evaluated by two experts. Results were statistically analysed. Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated. Tissues transported in milk showed poor preservation. Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

No MeSH data available.


Related in: MedlinePlus

Photomicrograph of the tissues fixed in ice, 30% jaggery, 20% honey, and milk for 1 hr, 6 hrs, 12 hrs, and 24 hrs, respectively (H&E, 10x).
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fig2: Photomicrograph of the tissues fixed in ice, 30% jaggery, 20% honey, and milk for 1 hr, 6 hrs, 12 hrs, and 24 hrs, respectively (H&E, 10x).

Mentions: Among the four transport media, tissues preserved in 30% jaggery elicited superior morphology followed by 20% honey and ice. The photomicrographs of H&E stained soft tissue specimens are depicted in Figure 2. The results obtained by Kruskal Wallis ANOVA test were depicted in Table 2.


Instant transport media for biopsied soft tissue specimens: a comparative study.

Patil S, Rao RS, Agarwal A, Raj AT - Scientifica (Cairo) (2015)

Photomicrograph of the tissues fixed in ice, 30% jaggery, 20% honey, and milk for 1 hr, 6 hrs, 12 hrs, and 24 hrs, respectively (H&E, 10x).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352508&req=5

fig2: Photomicrograph of the tissues fixed in ice, 30% jaggery, 20% honey, and milk for 1 hr, 6 hrs, 12 hrs, and 24 hrs, respectively (H&E, 10x).
Mentions: Among the four transport media, tissues preserved in 30% jaggery elicited superior morphology followed by 20% honey and ice. The photomicrographs of H&E stained soft tissue specimens are depicted in Figure 2. The results obtained by Kruskal Wallis ANOVA test were depicted in Table 2.

Bottom Line: Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated.Tissues transported in milk showed poor preservation.Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology & Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Bangalore 560054, India.

ABSTRACT
Background. Formalin, a traditionally preferred fixative in the field of pathology, has restricted usage in private settings. Since its toxicity violates the Occupational Safety and Health Administration regulations, an eco-friendly alternative would be the need of the hour. Hence an instant media which is economical and nontoxic and enables easy transport of biopsied soft tissue specimens in its original state is of vital importance. Materials and Methods. Commercially available fresh goat buccal mucosa specimens were sliced into smaller bits of equal dimensions and placed in six different containers containing 20% honey, 30% jaggery, milk, and ice for 1 hr, 6 hours, 12 hours, and 24 hours each with formalin as a positive control. After the set time interval, the specimens were fixed in 10% buffered formalin for 24 hours followed by routine processing and staining. The histologic interpretations were a blinded procedure and evaluated by two experts. Results were statistically analysed. Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated. Tissues transported in milk showed poor preservation. Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

No MeSH data available.


Related in: MedlinePlus