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Instant transport media for biopsied soft tissue specimens: a comparative study.

Patil S, Rao RS, Agarwal A, Raj AT - Scientifica (Cairo) (2015)

Bottom Line: Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated.Tissues transported in milk showed poor preservation.Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology & Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Bangalore 560054, India.

ABSTRACT
Background. Formalin, a traditionally preferred fixative in the field of pathology, has restricted usage in private settings. Since its toxicity violates the Occupational Safety and Health Administration regulations, an eco-friendly alternative would be the need of the hour. Hence an instant media which is economical and nontoxic and enables easy transport of biopsied soft tissue specimens in its original state is of vital importance. Materials and Methods. Commercially available fresh goat buccal mucosa specimens were sliced into smaller bits of equal dimensions and placed in six different containers containing 20% honey, 30% jaggery, milk, and ice for 1 hr, 6 hours, 12 hours, and 24 hours each with formalin as a positive control. After the set time interval, the specimens were fixed in 10% buffered formalin for 24 hours followed by routine processing and staining. The histologic interpretations were a blinded procedure and evaluated by two experts. Results were statistically analysed. Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated. Tissues transported in milk showed poor preservation. Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

No MeSH data available.


Related in: MedlinePlus

Flowchart representing the protocol employed in the study.
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Related In: Results  -  Collection


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fig1: Flowchart representing the protocol employed in the study.

Mentions: The study was done to assess and compare the quality of the biopsied soft tissues preserved in various transport media for set time intervals (1 hour (hr), 6 hrs, 12 hrs, and 24 hrs) with formalin as a positive control. Commercially available fresh goat buccal mucosa specimens (n = 80) were cut into smaller bits measuring approximately 0.5 × 0.5 × 0.5 cm each. 5 tissue bits were placed in six different containers each containing 10% buffered formalin, distilled water, 20% honey, 30% jaggery, milk, and ice for specific amounts of time intervals, namely 1 hr, 6 hrs, 12 hrs, and 24 hrs. Formalin was taken as the positive control and distilled water as negative control. The solutions were at room temperature, except milk which had to be stored in the refrigerator at 5 degrees Celsius. After the set time periods the tissues were removed from the respective transport media and fixed in 10% buffered formalin for another 24 hrs, following which standard processing and H&E staining were undertaken. Figure 1 summarises the entire procedure. The histologic sections were assessed for histomorphological criteria enlisted in Table 1. The criteria were set on a scale of 1–4 (1 being poor and 4 being excellent) adapted from Patil et al. [5]. Two experts were used to assess the histomorphological features and the whole procedure was blinded. The scores were analysed using Kruskal Wallis ANOVA test. Kappa statistics were carried out for inter observer variability.


Instant transport media for biopsied soft tissue specimens: a comparative study.

Patil S, Rao RS, Agarwal A, Raj AT - Scientifica (Cairo) (2015)

Flowchart representing the protocol employed in the study.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352508&req=5

fig1: Flowchart representing the protocol employed in the study.
Mentions: The study was done to assess and compare the quality of the biopsied soft tissues preserved in various transport media for set time intervals (1 hour (hr), 6 hrs, 12 hrs, and 24 hrs) with formalin as a positive control. Commercially available fresh goat buccal mucosa specimens (n = 80) were cut into smaller bits measuring approximately 0.5 × 0.5 × 0.5 cm each. 5 tissue bits were placed in six different containers each containing 10% buffered formalin, distilled water, 20% honey, 30% jaggery, milk, and ice for specific amounts of time intervals, namely 1 hr, 6 hrs, 12 hrs, and 24 hrs. Formalin was taken as the positive control and distilled water as negative control. The solutions were at room temperature, except milk which had to be stored in the refrigerator at 5 degrees Celsius. After the set time periods the tissues were removed from the respective transport media and fixed in 10% buffered formalin for another 24 hrs, following which standard processing and H&E staining were undertaken. Figure 1 summarises the entire procedure. The histologic sections were assessed for histomorphological criteria enlisted in Table 1. The criteria were set on a scale of 1–4 (1 being poor and 4 being excellent) adapted from Patil et al. [5]. Two experts were used to assess the histomorphological features and the whole procedure was blinded. The scores were analysed using Kruskal Wallis ANOVA test. Kappa statistics were carried out for inter observer variability.

Bottom Line: Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated.Tissues transported in milk showed poor preservation.Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Pathology & Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Bangalore 560054, India.

ABSTRACT
Background. Formalin, a traditionally preferred fixative in the field of pathology, has restricted usage in private settings. Since its toxicity violates the Occupational Safety and Health Administration regulations, an eco-friendly alternative would be the need of the hour. Hence an instant media which is economical and nontoxic and enables easy transport of biopsied soft tissue specimens in its original state is of vital importance. Materials and Methods. Commercially available fresh goat buccal mucosa specimens were sliced into smaller bits of equal dimensions and placed in six different containers containing 20% honey, 30% jaggery, milk, and ice for 1 hr, 6 hours, 12 hours, and 24 hours each with formalin as a positive control. After the set time interval, the specimens were fixed in 10% buffered formalin for 24 hours followed by routine processing and staining. The histologic interpretations were a blinded procedure and evaluated by two experts. Results were statistically analysed. Results. 30% jaggery proved to be an ideal transport media showing high quality preservation after 24 hours. 20% honey and ice showed optimal tissue preservation up to 6 hours following which quality deteriorated. Tissues transported in milk showed poor preservation. Conclusion. 30% jaggery can be endorsed in routine histopathological analysis as a transport media.

No MeSH data available.


Related in: MedlinePlus