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Transplanted human umbilical cord mesenchymal stem cells facilitate lesion repair in B6.Fas mice.

Ruan GP, Yao X, Yang SJ, Wang JX, Shu F, Li ZA, Liu JF, Pang RQ, Pan XH - J Immunol Res (2014)

Bottom Line: No effective treatment for SLE currently exists.After four rounds of cell transplantation, we observed a statistically significant decrease in the levels of mouse anti-nuclear, anti-histone, and anti-double-stranded DNA antibodies in transplanted mice compared with controls.The percentage of CD4(+)CD25(+)Foxp3(+) T cells in mouse peripheral blood significantly increased after H-UC-MSC transplantation.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.

ABSTRACT

Background: Systemic lupus erythematosus (SLE) is a multisystem disease that is characterized by the appearance of serum autoantibodies. No effective treatment for SLE currently exists.

Methods: We used human umbilical cord mesenchymal stem cell (H-UC-MSC) transplantation to treat B6.Fas mice.

Results: After four rounds of cell transplantation, we observed a statistically significant decrease in the levels of mouse anti-nuclear, anti-histone, and anti-double-stranded DNA antibodies in transplanted mice compared with controls. The percentage of CD4(+)CD25(+)Foxp3(+) T cells in mouse peripheral blood significantly increased after H-UC-MSC transplantation.

Conclusions: The results showed that H-UC-MSCs could repair lesions in B6.Fas mice such that all of the relevant disease indicators in B6.Fas mice were restored to the levels observed in normal C57BL/6 mice.

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Related in: MedlinePlus

(a–e) Spleen H&E staining (200x magnification). (a) C57BL/6 mouse normal control group. (b) B6.Fas mouse model group. The arrow indicates megakaryocytes. (c) B6.Fas mouse low-dose group. (d) B6.Fas mouse medium-dose group. (e) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible hemosiderin staining, which improved after treatment. (f–j) Liver H&E staining (200x magnification). (f) C57BL/6 mouse normal control group. (g) B6.Fas mouse model group. The arrow shows necrotic liver cells. (h) B6.Fas mouse low-dose group. (i) B6.Fas mouse medium-dose group. (j) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible spots of necrotic liver cells, which improved after treatment. (k–o) Kidney H&E staining (200x magnification). (k) C57BL/6 mouse normal control group. (l) B6.Fas mouse model group. The arrow shows reduced renal cysts. (m) B6.Fas mouse low-dose group. (n) B6.Fas mouse medium-dose group. (o) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed slightly proliferative mesangial cells and reduced renal cysts, which improved after treatment. (p–t) Lung H&E staining (200x magnification). (p) C57BL/6 mouse normal control group. (q) B6.Fas mouse model group. The arrow shows inflammatory cell infiltration. B6.Fas mouse low-dose group. (s) B6.Fas mouse medium-dose group. (t) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible alveolar septal infiltration of inflammatory cells, which improved after treatment. (u–y) Renal Masson staining. (u) C57BL/6 mouse normal control group (200x magnification). (v) B6.Fas mouse model group (400x magnification). The arrow shows fibrosis of the tubulointerstitial renal cortex. (w) B6.Fas mouse low-dose group (200x magnification). (x) B6.Fas mouse medium-dose group (400x magnification). (y) B6.Fas mouse high-dose group (200x magnification). The B6.Fas mouse model group displayed slight fibrosis of the tubulointerstitial renal cortex, which improved after treatment. Arrows show the abnormal area.
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fig3: (a–e) Spleen H&E staining (200x magnification). (a) C57BL/6 mouse normal control group. (b) B6.Fas mouse model group. The arrow indicates megakaryocytes. (c) B6.Fas mouse low-dose group. (d) B6.Fas mouse medium-dose group. (e) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible hemosiderin staining, which improved after treatment. (f–j) Liver H&E staining (200x magnification). (f) C57BL/6 mouse normal control group. (g) B6.Fas mouse model group. The arrow shows necrotic liver cells. (h) B6.Fas mouse low-dose group. (i) B6.Fas mouse medium-dose group. (j) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible spots of necrotic liver cells, which improved after treatment. (k–o) Kidney H&E staining (200x magnification). (k) C57BL/6 mouse normal control group. (l) B6.Fas mouse model group. The arrow shows reduced renal cysts. (m) B6.Fas mouse low-dose group. (n) B6.Fas mouse medium-dose group. (o) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed slightly proliferative mesangial cells and reduced renal cysts, which improved after treatment. (p–t) Lung H&E staining (200x magnification). (p) C57BL/6 mouse normal control group. (q) B6.Fas mouse model group. The arrow shows inflammatory cell infiltration. B6.Fas mouse low-dose group. (s) B6.Fas mouse medium-dose group. (t) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible alveolar septal infiltration of inflammatory cells, which improved after treatment. (u–y) Renal Masson staining. (u) C57BL/6 mouse normal control group (200x magnification). (v) B6.Fas mouse model group (400x magnification). The arrow shows fibrosis of the tubulointerstitial renal cortex. (w) B6.Fas mouse low-dose group (200x magnification). (x) B6.Fas mouse medium-dose group (400x magnification). (y) B6.Fas mouse high-dose group (200x magnification). The B6.Fas mouse model group displayed slight fibrosis of the tubulointerstitial renal cortex, which improved after treatment. Arrows show the abnormal area.

Mentions: The transplanted H-UC-MSCs improved the autoantibody levels most likely by inducing the clearance of the autoantibodies or by affecting the apoptosis defects described in the SLE model.


Transplanted human umbilical cord mesenchymal stem cells facilitate lesion repair in B6.Fas mice.

Ruan GP, Yao X, Yang SJ, Wang JX, Shu F, Li ZA, Liu JF, Pang RQ, Pan XH - J Immunol Res (2014)

(a–e) Spleen H&E staining (200x magnification). (a) C57BL/6 mouse normal control group. (b) B6.Fas mouse model group. The arrow indicates megakaryocytes. (c) B6.Fas mouse low-dose group. (d) B6.Fas mouse medium-dose group. (e) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible hemosiderin staining, which improved after treatment. (f–j) Liver H&E staining (200x magnification). (f) C57BL/6 mouse normal control group. (g) B6.Fas mouse model group. The arrow shows necrotic liver cells. (h) B6.Fas mouse low-dose group. (i) B6.Fas mouse medium-dose group. (j) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible spots of necrotic liver cells, which improved after treatment. (k–o) Kidney H&E staining (200x magnification). (k) C57BL/6 mouse normal control group. (l) B6.Fas mouse model group. The arrow shows reduced renal cysts. (m) B6.Fas mouse low-dose group. (n) B6.Fas mouse medium-dose group. (o) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed slightly proliferative mesangial cells and reduced renal cysts, which improved after treatment. (p–t) Lung H&E staining (200x magnification). (p) C57BL/6 mouse normal control group. (q) B6.Fas mouse model group. The arrow shows inflammatory cell infiltration. B6.Fas mouse low-dose group. (s) B6.Fas mouse medium-dose group. (t) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible alveolar septal infiltration of inflammatory cells, which improved after treatment. (u–y) Renal Masson staining. (u) C57BL/6 mouse normal control group (200x magnification). (v) B6.Fas mouse model group (400x magnification). The arrow shows fibrosis of the tubulointerstitial renal cortex. (w) B6.Fas mouse low-dose group (200x magnification). (x) B6.Fas mouse medium-dose group (400x magnification). (y) B6.Fas mouse high-dose group (200x magnification). The B6.Fas mouse model group displayed slight fibrosis of the tubulointerstitial renal cortex, which improved after treatment. Arrows show the abnormal area.
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Related In: Results  -  Collection

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fig3: (a–e) Spleen H&E staining (200x magnification). (a) C57BL/6 mouse normal control group. (b) B6.Fas mouse model group. The arrow indicates megakaryocytes. (c) B6.Fas mouse low-dose group. (d) B6.Fas mouse medium-dose group. (e) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible hemosiderin staining, which improved after treatment. (f–j) Liver H&E staining (200x magnification). (f) C57BL/6 mouse normal control group. (g) B6.Fas mouse model group. The arrow shows necrotic liver cells. (h) B6.Fas mouse low-dose group. (i) B6.Fas mouse medium-dose group. (j) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible spots of necrotic liver cells, which improved after treatment. (k–o) Kidney H&E staining (200x magnification). (k) C57BL/6 mouse normal control group. (l) B6.Fas mouse model group. The arrow shows reduced renal cysts. (m) B6.Fas mouse low-dose group. (n) B6.Fas mouse medium-dose group. (o) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed slightly proliferative mesangial cells and reduced renal cysts, which improved after treatment. (p–t) Lung H&E staining (200x magnification). (p) C57BL/6 mouse normal control group. (q) B6.Fas mouse model group. The arrow shows inflammatory cell infiltration. B6.Fas mouse low-dose group. (s) B6.Fas mouse medium-dose group. (t) B6.Fas mouse high-dose group. The B6.Fas mouse model group displayed visible alveolar septal infiltration of inflammatory cells, which improved after treatment. (u–y) Renal Masson staining. (u) C57BL/6 mouse normal control group (200x magnification). (v) B6.Fas mouse model group (400x magnification). The arrow shows fibrosis of the tubulointerstitial renal cortex. (w) B6.Fas mouse low-dose group (200x magnification). (x) B6.Fas mouse medium-dose group (400x magnification). (y) B6.Fas mouse high-dose group (200x magnification). The B6.Fas mouse model group displayed slight fibrosis of the tubulointerstitial renal cortex, which improved after treatment. Arrows show the abnormal area.
Mentions: The transplanted H-UC-MSCs improved the autoantibody levels most likely by inducing the clearance of the autoantibodies or by affecting the apoptosis defects described in the SLE model.

Bottom Line: No effective treatment for SLE currently exists.After four rounds of cell transplantation, we observed a statistically significant decrease in the levels of mouse anti-nuclear, anti-histone, and anti-double-stranded DNA antibodies in transplanted mice compared with controls.The percentage of CD4(+)CD25(+)Foxp3(+) T cells in mouse peripheral blood significantly increased after H-UC-MSC transplantation.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Engineering Laboratory of Yunnan Province, Kunming General Hospital of Chengdu Military Command, Kunming 650032, China.

ABSTRACT

Background: Systemic lupus erythematosus (SLE) is a multisystem disease that is characterized by the appearance of serum autoantibodies. No effective treatment for SLE currently exists.

Methods: We used human umbilical cord mesenchymal stem cell (H-UC-MSC) transplantation to treat B6.Fas mice.

Results: After four rounds of cell transplantation, we observed a statistically significant decrease in the levels of mouse anti-nuclear, anti-histone, and anti-double-stranded DNA antibodies in transplanted mice compared with controls. The percentage of CD4(+)CD25(+)Foxp3(+) T cells in mouse peripheral blood significantly increased after H-UC-MSC transplantation.

Conclusions: The results showed that H-UC-MSCs could repair lesions in B6.Fas mice such that all of the relevant disease indicators in B6.Fas mice were restored to the levels observed in normal C57BL/6 mice.

Show MeSH
Related in: MedlinePlus