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Lactic acid bacteria strains exert immunostimulatory effect on H. pylori-induced dendritic cells.

Wiese M, Eljaszewicz A, Helmin-Basa A, Andryszczyk M, Motyl I, Wieczyńska J, Gackowska L, Kubiszewska I, Januszewska M, Michałkiewicz J - J Immunol Res (2015)

Bottom Line: At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900.Conclusions.This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.

ABSTRACT
The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.

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Related in: MedlinePlus

The cytokine IL-10/IL-12p70 ratio. Values expressed as medians from the ratios from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; and H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, stimulators versus L. paracasei 915; P < 0.05; DCs: dendritic cells.
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fig3: The cytokine IL-10/IL-12p70 ratio. Values expressed as medians from the ratios from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; and H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, stimulators versus L. paracasei 915; P < 0.05; DCs: dendritic cells.

Mentions: Next, we calculated the IL-10/IL-12p70 ratios obtained from these studies (Figure 3). These allowed the ranking of the strains from an “anti-inflammatory” to a “proinflammatory” profile. The strains L. paracasei 915 and H. pylori were classified as more anti-inflammatory. L. rhamnosus 900 showed a slightly proinflammatory profile with a very low IL-10/IL-12p70 ratio. The mixture L. paracasei 915 + H. pylori showed strong anti-inflammatory capability. In contrast, despite the rather high ratio of IL-10/IL-12p70, the mixture L. rhamnosus 900 + H. pylori did not show differences between stimulators.


Lactic acid bacteria strains exert immunostimulatory effect on H. pylori-induced dendritic cells.

Wiese M, Eljaszewicz A, Helmin-Basa A, Andryszczyk M, Motyl I, Wieczyńska J, Gackowska L, Kubiszewska I, Januszewska M, Michałkiewicz J - J Immunol Res (2015)

The cytokine IL-10/IL-12p70 ratio. Values expressed as medians from the ratios from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; and H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, stimulators versus L. paracasei 915; P < 0.05; DCs: dendritic cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352478&req=5

fig3: The cytokine IL-10/IL-12p70 ratio. Values expressed as medians from the ratios from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; and H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, stimulators versus L. paracasei 915; P < 0.05; DCs: dendritic cells.
Mentions: Next, we calculated the IL-10/IL-12p70 ratios obtained from these studies (Figure 3). These allowed the ranking of the strains from an “anti-inflammatory” to a “proinflammatory” profile. The strains L. paracasei 915 and H. pylori were classified as more anti-inflammatory. L. rhamnosus 900 showed a slightly proinflammatory profile with a very low IL-10/IL-12p70 ratio. The mixture L. paracasei 915 + H. pylori showed strong anti-inflammatory capability. In contrast, despite the rather high ratio of IL-10/IL-12p70, the mixture L. rhamnosus 900 + H. pylori did not show differences between stimulators.

Bottom Line: At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900.Conclusions.This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.

ABSTRACT
The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.

Show MeSH
Related in: MedlinePlus