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Lactic acid bacteria strains exert immunostimulatory effect on H. pylori-induced dendritic cells.

Wiese M, Eljaszewicz A, Helmin-Basa A, Andryszczyk M, Motyl I, Wieczyńska J, Gackowska L, Kubiszewska I, Januszewska M, Michałkiewicz J - J Immunol Res (2015)

Bottom Line: At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900.Conclusions.This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.

ABSTRACT
The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.

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Related in: MedlinePlus

Effect of the examined bacteria and their mixtures on the production of cytokines by DCs population. Values expressed as medians from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, §: stimulators versus L. rhamnosus 900 + H. pylori, and $: stimulators versus L. paracasei 915 + H. pylori; P < 0.05; DCs: dendritic cells.
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fig2: Effect of the examined bacteria and their mixtures on the production of cytokines by DCs population. Values expressed as medians from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, §: stimulators versus L. rhamnosus 900 + H. pylori, and $: stimulators versus L. paracasei 915 + H. pylori; P < 0.05; DCs: dendritic cells.

Mentions: The DCs were stimulated for 24 h with live bacteria, either a single strain or a mixture of two bacterial strains (Figure 2). All the stimulators effectively induced cytokine synthesis (IL-10, IL-12p70, IL-23, and TNF-α) when compared with control DCs (unstimulated DCs).


Lactic acid bacteria strains exert immunostimulatory effect on H. pylori-induced dendritic cells.

Wiese M, Eljaszewicz A, Helmin-Basa A, Andryszczyk M, Motyl I, Wieczyńska J, Gackowska L, Kubiszewska I, Januszewska M, Michałkiewicz J - J Immunol Res (2015)

Effect of the examined bacteria and their mixtures on the production of cytokines by DCs population. Values expressed as medians from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, §: stimulators versus L. rhamnosus 900 + H. pylori, and $: stimulators versus L. paracasei 915 + H. pylori; P < 0.05; DCs: dendritic cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352478&req=5

fig2: Effect of the examined bacteria and their mixtures on the production of cytokines by DCs population. Values expressed as medians from six independent experiments and interquartile ranges [Q1–Q3]; control: unstipulated DCs; L.r.: L. rhamnosus 900; L.p.: L. paracasei 915; H.p.: H. pylori; statistically significant differences are given as follows: ∗: stimulators versus control (unstimulated DCs), †: stimulators versus H. pylori, ‡: stimulators versus L. rhamnosus 900, §: stimulators versus L. rhamnosus 900 + H. pylori, and $: stimulators versus L. paracasei 915 + H. pylori; P < 0.05; DCs: dendritic cells.
Mentions: The DCs were stimulated for 24 h with live bacteria, either a single strain or a mixture of two bacterial strains (Figure 2). All the stimulators effectively induced cytokine synthesis (IL-10, IL-12p70, IL-23, and TNF-α) when compared with control DCs (unstimulated DCs).

Bottom Line: At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900.Conclusions.This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori.

View Article: PubMed Central - PubMed

Affiliation: Collegium Medicum, Nicolaus Copernicus University, M. Sklodowskiej-Curie 9 Street, 85-094 Bydgoszcz, Poland.

ABSTRACT
The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic against H. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns. Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains of L. rhamnosus 900 or L. paracasei 915 (antagonistic and nonantagonistic to H. pylori, resp.), in the presence or absence of H. pylori strain cagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production. Results. We observed that the LAB strains and the mixtures of LAB with H. pylori are able to induce mature DCs. At the same time, the L. paracasei 915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast to L. rhamnosus 900. Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation than H. pylori. Interestingly from the two chosen LAB strains the antagonistic to H. pylori-L. rhamnosus strain 900 has more proinflammatory and probably antibactericidal properties.

Show MeSH
Related in: MedlinePlus